1. Antibiotic Treatment in an Animal Model of Inflammatory Lung Disease.
- Author
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Cait A, Messing M, Cait J, Canals Hernaez D, and McNagny KM
- Subjects
- Animals, Animals, Newborn, Asthma chemically induced, Asthma genetics, Asthma microbiology, B-Lymphocytes drug effects, B-Lymphocytes immunology, B-Lymphocytes pathology, Bronchoalveolar Lavage Fluid chemistry, Bronchoalveolar Lavage Fluid immunology, Bronchoalveolar Lavage Fluid microbiology, Dendritic Cells drug effects, Dendritic Cells immunology, Dendritic Cells pathology, Disease Models, Animal, Eosine Yellowish-(YS) chemistry, Female, Hematoxylin chemistry, Humans, Immunoglobulin E genetics, Immunoglobulin E immunology, Interleukin-13 genetics, Interleukin-13 immunology, Interleukin-4 genetics, Interleukin-4 immunology, Interleukin-5 genetics, Interleukin-5 immunology, Lung pathology, Macrophages, Alveolar immunology, Macrophages, Alveolar pathology, Male, Mice, Mice, Inbred C57BL, Papain administration & dosage, T-Lymphocytes drug effects, T-Lymphocytes immunology, T-Lymphocytes pathology, Anti-Bacterial Agents pharmacology, Asthma immunology, Macrophages, Alveolar drug effects, Microbiota drug effects, Staining and Labeling methods, Vancomycin pharmacology
- Abstract
Allergic disease is on the rise and yet the underlying cause and risk factors are not fully understood. While lifesaving in many circumstances, the use of antibiotics and the subsequent disruption of the microbiome are positively correlated with the development of allergies. Here, we describe the use of the antibiotic vancomycin in combination with the papain-induced mouse model of allergic disease that allows for the assessment of microbiome perturbations and the impact on allergy development.
- Published
- 2021
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