1. Use of expressed sequence tag analysis and cDNA microarrays of the filamentous fungus Aspergillus nidulans.
- Author
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Sims AH, Robson GD, Hoyle DC, Oliver SG, Turner G, Prade RA, Russell HH, Dunn-Coleman NS, and Gent ME
- Subjects
- Aspergillus nidulans metabolism, Culture Media chemistry, DNA, Fungal isolation & purification, Ethanol metabolism, Fungal Proteins genetics, Fungal Proteins physiology, Gene Expression Regulation, Fungal, Gene Library, Genes, Fungal, Gluconeogenesis genetics, Glyoxylates metabolism, Reproducibility of Results, Aspergillus nidulans genetics, Aspergillus nidulans growth & development, Expressed Sequence Tags, Gene Expression Profiling methods, Glucose metabolism, Oligonucleotide Array Sequence Analysis
- Abstract
The use of microarrays in the analysis of gene expression is becoming widespread for many organisms, including yeast. However, although the genomes of a number of filamentous fungi have been fully or partially sequenced, microarray analysis is still in its infancy in these organisms. Here, we describe the construction and validation of microarrays for the fungus Aspergillus nidulans using PCR products from a 4092 EST conidial germination library. An experiment was designed to validate these arrays by monitoring the expression profiles of known genes following the addition of 1% (w/v) glucose to wild-type A. nidulans cultures grown to mid-exponential phase in Vogel's minimal medium with ethanol as the sole carbon source. The profiles of genes showing statistically significant differential expression following the glucose up-shift are presented and an assessment of the quality and reproducibility of the A. nidulans arrays discussed.
- Published
- 2004
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