1. Effect of Aspergillus flavus Fungal Elicitor on the Production of Terpenoid Indole Alkaloids in Catharanthus roseus Cambial Meristematic Cells.
- Author
-
Liang C, Chen C, Zhou P, Xu L, Zhu J, Liang J, Zi J, and Yu R
- Subjects
- Catharanthus chemistry, Catharanthus cytology, Catharanthus genetics, Chromatography, High Pressure Liquid, Gene Expression Regulation, Plant, Meristem cytology, Meristem metabolism, Plant Cells drug effects, Plant Cells metabolism, Reverse Transcriptase Polymerase Chain Reaction, Secologanin Tryptamine Alkaloids analysis, Tandem Mass Spectrometry, Aspergillus flavus chemistry, Catharanthus metabolism, Secologanin Tryptamine Alkaloids metabolism
- Abstract
This study reported the inducing effect of Aspergillus flavus fungal elicitor on biosynthesis of terpenoid indole alkaloids (TIAs) in Catharanthus roseus cambial meristematic cells (CMCs) and its inducing mechanism. According to the results determined by HPLC and HPLC-MS/MS, the optimal condition of the A. flavus elicitor was as follows: after suspension culture of C. roseus CMCs for 6 day, 25 mg/L A. flavus mycelium elicitor were added, and the CMC suspensions were further cultured for another 48 h. In this condition, the contents of vindoline, catharanthine, and ajmaline were 1.45-, 3.29-, and 2.14-times as high as those of the control group, respectively. Transcriptome analysis showed that D4H , G10H , GES , IRS , LAMT , SGD , STR , TDC , and ORCA3 were involved in the regulation of this induction process. The results of qRT-PCR indicated that the increasing accumulations of vindoline, catharanthine, and ajmaline in C. roseus CMCs were correlated with the increasing expression of the above genes. Therefore, A. flavus fungal elicitor could enhance the TIA production of C. roseus CMCs, which might be used as an alternative biotechnological resource for obtaining bioactive alkaloids.
- Published
- 2018
- Full Text
- View/download PDF