1. Effect of Various Compounds Blocking the Colony Pigmentation on the Aflatoxin B1 Production by Aspergillus flavus.
- Author
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Dzhavakhiya VG, Voinova TM, Popletaeva SB, Statsyuk NV, Limantseva LA, and Shcherbakova LA
- Subjects
- Aflatoxin B1 analysis, Antifungal Agents pharmacology, Aspergillus flavus drug effects, Aspergillus flavus growth & development, Benzaldehydes pharmacology, Fluconazole pharmacology, Lovastatin pharmacology, Mycelium drug effects, Mycelium growth & development, Mycelium metabolism, Thymol pharmacology, Triticum chemistry, Zea mays chemistry, Aflatoxin B1 biosynthesis, Aspergillus flavus metabolism, Lovastatin analogs & derivatives, Melanins biosynthesis, Pigmentation drug effects
- Abstract
Aflatoxins and melanins are the products of a polyketide biosynthesis. In this study, the search of potential inhibitors of the aflatoxin B1 (AFB1) biosynthesis was performed among compounds blocking the pigmentation in fungi. Four compounds-three natural (thymol, 3-hydroxybenzaldehyde, compactin) and one synthetic (fluconazole)-were examined for their ability to block the pigmentation and AFB1 production in Aspergillus flavus . All compounds inhibited the mycelium pigmentation of a fungus growing on solid medium. At the same time, thymol, fluconazole, and 3-hydroxybenzaldehyde stimulated AFB1 accumulation in culture broth of A. flavus under submerged fermentation, whereas the addition of 2.5 μg/mL of compactin resulted in a 50× reduction in AFB1 production. Moreover, compactin also suppressed the sporulation of A. flavus on solid medium. In vivo treatment of corn and wheat grain with compactin (50 μg/g of grain) reduced the level of AFB1 accumulation 14 and 15 times, respectively. Further prospects of the compactin study as potential AFB1 inhibitor are discussed., Competing Interests: The authors declare no conflict of interest.
- Published
- 2016
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