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Start Over Author "Nogueira, Elisa" Topic artificial metalloenzymes
1 results on '"Nogueira, Elisa"'

1. High-level secretion of recombinant full-length streptavidin in Pichia pastoris and its application to enantioselective catalysis.

Author

Nogueira, Elisa S., Schleier, Thomas, Dürrenberger, Marc, Ballmer-Hofer, Kurt, Ward, Thomas R., and Jaussi, Rolf

Subjects
*SECRETION, *RECOMBINANT proteins, *STREPTAVIDIN, *PICHIA pastoris, *ENANTIOSELECTIVE catalysis, *BIOTIN, *ORGANOMETALLIC compounds
Abstract

Highlights: [•] Biotin-free streptavidin was successfully expressed as a secretory protein by Pichia pastoris. [•] The final yield was approximately 650mg/L of streptavidin by non-optimized fed-batch fermentation. [•] Streptavidin from Pichia pastoris binds the organometallic catalyst moiety. [•] Streptavidin produced by Pichia pastoris functions as artificial imine reductase. [ABSTRACT FROM AUTHOR]

Published
2014
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