1. Seropositivity and determinants of immunoglobulin-G (IgG) antibodies against Herpes simplex virus (HSV) types -1 and -2 in pregnant women in Port Harcourt, Nigeria
- Author
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Livia Melo Villar, Ngwena Ag, Sabina Berceanu, Cookey Ti, Monica Cirstoiu, Degu G, Castaneda Jc, Oscar Peralta-Zaragoza, Glueck R, Helena Medina Cruz, Gupta G, Were Eo, Paredes-Cervantes, Padilla-Quirarte Ho, Elvira Brătilă, Kokeb M, Garcia-Miranda La, Alex Emilian Stepan, Palma-Irizarry M, Ciortea R, Okonko Io, Ana I. Burguete-García, Bermudez-Morales Vh, Serrano H, Ndede I, MehedinTu C, Costin Berceanu, Kirvis Torres-Poveda, Flores Gl, Hernandez-Marquez E, de Almeida Aj, Mining Sk, Garcia-Suarez, Olamijulo Ja, Vanessa Alves Marques, Rishi N, Trejo-Moreno C, Fierros-Zarate G, Juliana Custódio Miguel, Lia Laura Lewis-Ximenez, Moyra Machado Portilho, Olaleye O, Pliego-Rivero Fb, Emonyi Wi, Contreras I, Estrada Ja, Gheonea Ia, Elisabeth Lampe, Scalioni Lde P, and Madrid-Marina
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Adult ,Cost effectiveness ,Herpesvirus 2, Human ,Lymphocyte ,Population ,Nigeria ,Enzyme-Linked Immunosorbent Assay ,Herpesvirus 1, Human ,Antibodies, Viral ,Young Adult ,Antigen ,Pregnancy ,Seroepidemiologic Studies ,Pelvic inflammatory disease ,Prevalence ,Humans ,Medicine ,education ,education.field_of_study ,Herpes Genitalis ,Marital Status ,biology ,medicine.diagnostic_test ,business.industry ,Complete blood count ,Herpes Simplex ,Articles ,General Medicine ,medicine.anatomical_structure ,Immunoglobulin G ,Immunology ,biology.protein ,Female ,BMSH, determinants, ELISA, HSV-1, HSV-2, IgG, seropositivity ,Antibody ,business ,Viral load - Abstract
Background: The basis of apparent resistance to HIV-1 infection by some HIV-1 exposed heterosexual couples is unclear. The nature and functional characteristics of their immune cells could offer insight. Objective: To characterise immune profile in terms of CD8+ CD4+ CD56+/16+ and CD19+ cell number and Th1/Th2 cytokine expression in heterosexual HIV–1 discordant couples. Methods: Thirty-three (33) untreated HIV-1 discordant (index with CD4³250 cells/µL) concordant positive and negative control couples were recruited at MTRH/USAID-AMPATH partnership clinics in Western Kenya. Lymphocyte phenotypes complete blood count viral load (VL) and Th1/Th2 cytokine levels were determined and compared between the study groups. Results: Differences were observed between: HIV-1 discordant index females and concordant positive females CD8+ 711.0 cells/ml vs 1070.8 cells/ml (p = 0.005); %CD8+ 43.0% vs 56.3%;( p = 0.017); CD4+ 28.0% vs 18.3% (p = 0.02); CD56+/16+ 208.0 cells/ml vs 447.5 cells/ml (p = .011); discordant index males and concordant negative males CD4+ 700.7cell/ml vs 1000.4 cells/ml (p = 0.003); CD8+ 554.7 cells/ml vs 1062.4 cells/ml (p = 0.002); CD56+/16+ 290.5 cells/ml vs 450.7 cells/ml (p = 0.027). Uninfected female partners had higher mean cells/µL and percentage CD19+ than index females. In discordant index log10 viral load as negatively correlated with CD4+ (rho = -0.274) and CD56+/16+ (rho = -0.319). No Th1 to Th2 cytokine shift occurred in both index and partners of discordant couples. Conclusion: Lymphocyte subsets counts and cytokine levels correlate with HIV-1 resistance among the studied heterosexual HIV-1 discordant couples. Studies using HIV-1 specific antigens and lymphocytes from similar cohorts are necessary to improve understanding the role of immune response in HIV-1 discordance.
- Published
- 2015
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