1. Transcriptome and chromatin landscape of iNKT cells are shaped by subset differentiation and antigen exposure
- Author
-
Pandurangan Vijayanand, Mitchell Kronenberg, Ashu Sethi, Mallory Paynich Murray, Jason A. Greenbaum, Ashmitaa Logandha Ramamoorthy Premlal, Sara Herrera-de la Mata, Isaac Engel, James P. Scott-Browne, Sandy Lucette Rosales, Grégory Seumois, and Goo-Young Seo
- Subjects
0301 basic medicine ,Cellular immunity ,T Follicular Helper Cells ,Science ,Immunology ,General Physics and Astronomy ,Mice, Transgenic ,Innate lymphoid cells ,Thymus Gland ,Biology ,Lymphocyte Activation ,Article ,General Biochemistry, Genetics and Molecular Biology ,Chromatin remodeling ,Transcriptome ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Antigen ,T-Lymphocyte Subsets ,Transcription (biology) ,medicine ,Animals ,Lymphocytes ,Lung ,Gene ,Epigenomics ,Multidisciplinary ,Effector ,Innate lymphoid cell ,Cell Differentiation ,General Chemistry ,Phenotype ,Chromatin ,Cell biology ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,Natural Killer T-Cells ,Female ,030215 immunology - Abstract
Invariant natural killer T cells (iNKT cells) differentiate into thymic and peripheral NKT1, NKT2 and NKT17 subsets. Here we use RNA-seq and ATAC-seq analyses and show iNKT subsets are similar, regardless of tissue location. Lung iNKT cell subsets possess the most distinct location-specific features, shared with other innate lymphocytes in the lung, possibly consistent with increased activation. Following antigenic stimulation, iNKT cells undergo chromatin and transcriptional changes delineating two populations: one similar to follicular helper T cells and the other NK or effector like. Phenotypic analysis indicates these changes are observed long-term, suggesting that iNKT cells gene programs are not fixed, but they are capable of chromatin remodeling after antigen to give rise to additional subsets., Invariant natural killer T cells are known to be composed of a number of phenotypic and functionally distinct populations. Here the authors use transcriptomic and epigenomic analysis to further characterize the peripheral iNKT compartment before and after antigenic stimulation.
- Published
- 2021