1. The effect of a single, temperature-sensitive mutation on global gene expression in Escherichia coli
- Author
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Yong Li, Sidney Altman, and Kyle Cole
- Subjects
RNase P ,Pair-rule gene ,Biology ,Ribonuclease P ,Article ,Substrate Specificity ,Bacterial Proteins ,Endoribonucleases ,Gene expression ,Gene cluster ,Escherichia coli ,Point Mutation ,RNA, Catalytic ,Molecular Biology ,Gene ,Oligonucleotide Array Sequence Analysis ,Regulator gene ,Genetics ,Escherichia coli Proteins ,Gene Expression Profiling ,Temperature ,Molecular biology ,Gene expression profiling ,Genes, Bacterial ,Multigene Family ,DNA microarray - Abstract
High-density DNA microarrays have been used to explore the genomic profiling of gene expression of a defective Escherichia coli strain with a temperature-sensitive mutation in the protein component of RNase P. A novel gene cluster was discovered in which two of the genes are known substrates of RNase P. The expression pattern of essential genes and gene discovery from intergenic regions, for which other new transcripts are found, are also discussed.
- Published
- 2003
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