1. Enhancement effect of dihydroartemisinin on human γδ T cell proliferation and killing pancreatic cancer cells.
- Author
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Zhou ZH, Chen FX, Xu WR, Qian H, Sun LQ, Lü XT, Chen L, Zhang J, Ji HC, and Fei SJ
- Subjects
- Adolescent, Adult, Cell Line, Tumor, Cell Proliferation drug effects, Cell Survival drug effects, Granzymes metabolism, Humans, Interferon-gamma metabolism, Lysosomal-Associated Membrane Protein 1 metabolism, Pancreatic Neoplasms, Perforin metabolism, T-Lymphocyte Subsets metabolism, T-Lymphocytes metabolism, Young Adult, Adjuvants, Immunologic pharmacology, Antineoplastic Agents pharmacology, Artemisinins pharmacology, T-Lymphocyte Subsets drug effects, T-Lymphocytes drug effects
- Abstract
γδ T cells play important roles in innate immunity against tumors and infections. Inhibitory effect of dihydroartemisinin on growth of cancer cells has been found in recent years. In this study, we investigated the effect of dihydroartemisinin on human γδ T cell proliferation by MTT assay and killing activity against pancreatic cancer cells SW1990, BxPC-3 and PANC-1 by LDH release assay in vitro. Intracellular molecule alterations were verified by flow cytometry. The results suggested that appropriate concentration of dihydroartemisinin favored the expansion of γδ T cells and enhanced γδ T cell mediated killing activity against pancreatic cancer cells. Up-regulation of intracellular perforin, granzyme B expression and IFN-γ production may be the important mechanism of dihydroartemisinin on increased antitumor activity of γδ T cells., (© 2013. Published by Elsevier B.V. All rights reserved.)
- Published
- 2013
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