10 results on '"Bolt, Hermann M."'
Search Results
2. Synergism of aromatic amines and benzo[a]pyrene in induction of Ah receptor-dependent genes
- Author
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Borza, Alexandra, Plöttner, Sabine, Wolf, Alexander, Behm, Claudia, Selinski, Silvia, Hengstler, Jan G., Roos, Peter H., Bolt, Hermann M., Kuhlmann, Jürgen, and Föllmann, Wolfram
- Published
- 2008
- Full Text
- View/download PDF
Catalog
3. Occupational exposure and urological cancer
- Author
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Golka, Klaus, Wiese, Andreas, Assennato, Giorgio, and Bolt, Hermann M.
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- 2004
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4. Bladder Cancer in Crack Testers Applying Azo Dye-Based Sprays to Metal Bodies.
- Author
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Golka, Klaus, Kopps, Silke, Prager, Hans-Martin, Mende, Stephan v., Thiel, Ralf, Jungmann, Olaf, Zumbe, Jürgen, Bolt, Hermann M., Blaszkewicz, Meinolf, Hengstler, Jan G., and Selinski, Silvia
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BLADDER cancer ,AZO dyes ,AROMATIC amines ,OCCUPATIONAL diseases ,ACETYLATOR status (Pharmacology) ,NAPHTHYLAMINES - Abstract
Bladder cancer may be produced by azo dyes due to the presence of carcinogenic aromatic amines. Nine cases of suspected occupational bladder cancer that were exposed to different crack test sprays in metal-related jobs were examined. A detailed occupational history was taken and, if possible, the N-acetyltransferase 2 (NAT2) status was determined. The first exposure to crack test sprays ranged from 1957 to 1986. Age at first exposure was between 14 and 33 yr. Age at first diagnosis of bladder cancer varied from 35 to 64 yr. Latency periods were between 17 and 45 yr. The maximal reported exposure period was 29 yr. Four of six genotyped cases were slow NAT2 acetylators. The handling of the crack test spray included spraying the red dye-containing matter on the metal body and washing off the spray with a rag. Thus, workers were exposed by dermal contact as well as by inhalation. The crack test spray, which makes the cracks visible after washing off the red testing spray compounds and applying an additional white spray, contained dyes such as solvent red 19 (Sudan red 7B, N-ethyl-1[[4-(phenylazo)phenyl]azo]-2-naphthylamine) or a mixture of p-phenylazoaniline-N-ethyl-2-naphthylamine and p-phenylazoaniline-N-ethyl-1-naphthylamine. The aromatic amine 2-naphthylamine is classified as human carcinogen by IARC and the national authorities and has been banned in many countries since the mid 1950s. Bladder cancer patients with metal-related jobs need to be explicitly asked about the use of crack test sprays. [ABSTRACT FROM AUTHOR] more...
- Published
- 2012
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5. Glutathione S-Transferase P1 ILE105Val Polymorphism in Occupationally Exposed Bladder Cancer Cases.
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Kopps, Silke, Angeli-Greaves, Miriam, Blaszkewicz, Meinolf, Prager, Hans-Martin, Roemer, Hermann C., Löhlein, Dietrich, Weistenhöfer, Wobbeke, Bolt, Hermann M., and Golka, Klaus
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GENETIC polymorphisms ,GENETIC research ,POLYMORPHISM (Zoology) ,GLUTATHIONE ,AROMATIC amines ,POLYMERASE chain reaction ,DNA polymerases ,CANCER risk factors ,TUMORS - Abstract
The genotype glutathione S-transferase P1 (GSTP1) influences the risk for bladder cancer among Chinese workers occupationally exposed to benzidine. Studies of Caucasian bladder cancer cases without known occupational exposures showed conflicting results. Research was thus conducted to define the role of GSTP1 genotypes in Caucasian bladder cancer cases with an occupational history of exposure to aromatic amines. DNA from 143 cases reported to the Industrial Professional Associations (Berufsgenossenschaften) in Germany from 1996 to 2004, who had contracted urothelial cancer due to occupational exposure, and 196 patients from one Department of Surgery in Dortmund, without known malignancy in their medical history, were genotyped using real-time polymerase chain reaction (PCR) (LightCycler) in relation to GSTP1 A1578G (Ile105Val) polymorphism. Among the subjects with bladder cancer, 46% presented the AA genotype, 39% the AG genotype, and 15% the GG genotype. In the surgical (noncancer) control group analyzed, 42% presented the AA genotype, 42% the AG genotype, and 16% the GG genotype. A subgroup of bladder cancer cases, represented by 46 painters, showed a distribution of 41% of the AA genotype, 48% of the AG genotype, and 11% of the GG genotype. Data indicated that in Caucasians exposed to aromatic amines the GSTP1 A1578G polymorphism did not appear to play a significant role as a predisposing factor for bladder cancer incidence. [ABSTRACT FROM AUTHOR] more...
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- 2008
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6. Evaluation of Time Dependence and Interindividual Differences in Benzo[a]pyrene-Mediated CYP1A1 Induction and Genotoxicity in Porcine Urinary Bladder Cell Cultures.
- Author
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Plöttner, Sabine, Borza, Alexandra, Wolf, Alexander, Bolt, Hermann M., Kuhlmann, Jurgen, and Föllmann, Wolfram
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CANCER treatment ,CIGARETTE smokers ,AMINES ,TOBACCO ,DNA damage ,AROMATIC amines ,HAZARDOUS substances ,EPITHELIAL cells ,DNA polymerases - Abstract
Exposure to tobacco smoke is an established cause of cancer in humans and cigarette smoking is a risk factor for urinary bladder cancer development. Aromatic amines are believed responsible for the bladder-specific carcinogenic effect, but polycyclic aromatic hydrocarbons (PAHs) are also of potential relevance. Urothelial cells contain a number of xenobiotic-metabolizing enzymes, which enable them to convert pro-carcinogens into reactive intermediates. In a preceding study, it was demonstrated using cultured porcine urinary bladder epithelial cells (PUBEC) that CYP1A1 mRNA is induced in a potent manner by treatment with benzo[a]pyrene (BaP). In the present study, the time dependence of these effects was evaluated and whether PUBEC cultures derived from individual donors respond differently to BaP treatment was determined. CYP1A1 induction was analyzed by quantitative reverse-transcription polymerase chain reaction (RT-PCR), and genotoxic effects were studied using the Comet assay. Incubation of PUBEC with BaP increased CYP1A1 expression and induction of DNA strand breaks in a time-dependent manner. Interindividual differences were found between PUBEC cultures derived from several donor animals with respect to the response to BaP, such that the extent of CYP1A1 induction and magnitude of DNA damage was interrelated. Hence, individual differences in metabolic capacities and responsiveness to xenobiotics of urothelial cells from individual donors may be factors in susceptibility to genotoxic effects induced by PAHs. [ABSTRACT FROM AUTHOR] more...
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- 2008
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7. Elevated Bladder Cancer Risk Due to Colorants—A Statewide Case-Control Study in North Rhine-Westphalia, Germany.
- Author
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Golka, Klaus, Heitmann, Peter, Gieseler, Frank, Hodzic, Jasmin, Masche, Nicolas, Bolt, Hermann M., and Geller, Frank
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HAZARDOUS substances ,INDUSTRIAL safety ,BLADDER ,CANCER treatment ,URINARY organs ,AROMATIC amines ,AMINES - Abstract
Occupational exposure to aromatic amines is a known bladder cancer risk factor, whereas the impact of exposure to azo dyes, which may release aromatic amines in humans, is at present controversial. Therefore, the impact of occupational exposures to colorants was investigated in 156 bladder cancer cases and 336 controls in the state of North Rhine-Westphalia. All bladder cancer cases and controls (diagnosed with prostate cancer) requested after-care treatment. The subjects were investigated using a questionnaire for all occupations ever performed for more than 6 mo and for exposures to several possible occupational and nonoccupational bladder carcinogens. The relative bladder cancer risk was adjusted for age and smoking. The adjusted odds ratio (OR) for bladder cancer was elevated in 7 painters (OR 1.98, 95% CI 0.64-6.11), 4 hairdressers (OR 4.9, 95% CI 0.85-28.39), and 16 cases who reported a wood processing occupation (OR 1.19, 95% CI 0.58-2.41). Ten of these 16 cases reported chronic exposure to colorants (OR 1.84, 95% CI 0.68-4.95). The results of this epidemiological study confirm the hypothesis that individuals exposed to colorants show an elevated bladder cancer risk. [ABSTRACT FROM AUTHOR] more...
- Published
- 2008
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8. Reconstruction of N-acetyltransferase 2 haplotypes using PHASE.
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Golka, Klaus, Blaszkewicz, Meinolf, Samimi, Mirabutaleb, Bolt, Hermann M., and Selinski, Silvia
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ACETYLTRANSFERASES ,GENETIC polymorphisms ,CAUCASIAN race ,URINARY organs ,AROMATIC amines ,BLADDER cancer ,COMPUTER software ,PROBABILITY measures ,NITROAROMATIC compounds - Abstract
The genotyping of N-acetyltransferase 2 ( NAT2) by PCR/RFLP methods yields in a considerable percentage ambiguous results. To resolve this methodical problem a statistical approach was applied. PHASE v2.1.1, a statistical program for haplotype reconstruction was used to estimate haplotype pairs from NAT2 genotyping data, obtained by the analysis of seven single nucleotide polymorphisms relevant for Caucasians. In 1,011 out of 2,921 (35%) subjects the haplotype pairs were clearcut by the PCR/RFLP data only. For the majority of the data the applied method resulted in a multiplicity (2–4) of possible haplotype pairs. Haplotype reconstruction using PHASE v2.1.1 cleared this ambiguity in all cases but one, where an alternative haplotype pair was considered with a probability of 0.029. The estimation of the NAT2 haplotype is important because the assignment of the NAT2 alleles * 12A, * 12B, * 12C or * 13 to the rapid or slow NAT2 genotype has been discussed controversially. A clear assignment is indispensable in surveys of human bladder cancer caused by aromatic amine exposures. In conclusion, PHASE v2.1.1 software allowed an unambiguous haplotype reconstruction in 2,920 of 2,921 cases (>99.9%). [ABSTRACT FROM AUTHOR] more...
- Published
- 2008
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9. Susceptibility to urinary bladder cancer: relevance of rs9642880[T], GSTM1 0/0 and occupational exposure.
- Author
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Golka, Klaus, Hermes, Matthias, Selinski, Silvia, Blaszkewicz, Meinolf, Bolt, Hermann M., Roth, Gerhard, Dietrich, Holger, Prager, Hans-Martin, Ickstadt, Katja, and Hengstler, Jan G.
- Abstract
Recently, a genome-wide single nucleotide polymorphism association study has identified a sequence variant 30 kb upstream of the c-Myc gene (allele T of rs9642880) that confers susceptibility to bladder cancer. However, the role of exposure to bladder carcinogens has not been considered. This prompted us to analyse the relevance of this polymorphism in 515 bladder cancer cases and 893 controls where the quality and quantity of occupational exposure to bladder carcinogens has been documented. When we analysed a hospital-based case-control series not selected for occupational exposure, rs9642880[T] was influential, in contrast to GSTM1 0/0. However, in a case-control series of patients that have been occupationally exposed to aromatic amines and polycyclic aromatic hydrocarbons, rs9642880[T] was not influential but GSTM1 0/0 was significantly associated with bladder cancer risk. Therefore, the degree to which rs9642880[T] and GSTM1 0/0 confer susceptibility to urinary bladder cancer seems to depend on the extent of exposure to urinary bladder carcinogens. [ABSTRACT FROM AUTHOR] more...
- Published
- 2009
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10. The enhanced bladder cancer susceptibility of NAT2 slow acetylators towards aromatic amines: a review considering ethnic differences
- Author
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Golka, Klaus, Prior, Verena, Blaszkewicz, Meinolf, and Bolt, Hermann M.
- Subjects
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BLADDER cancer , *AROMATIC amines , *CHEMICAL workers - Abstract
Human bladder cancer may be caused by exposure to aromatic amines. The polymorphic enzyme N-acetyltransferase 2 (NAT2) is involved in the metabolism of these compounds. Two classical studies on chemical workers in Europe, exposed in the past to aromatic amines like benzidine, unambiguously showed that the slow acetylator status is a genetic risk factor for arylamine-induced bladder cancer. In the former benzidine industry in Huddington, Great Britain, 22 of 23 exposed cases with bladder cancer, but only 57% of 95 local controls without bladder cancer were of the slow acetylator phenotype. In Leverkusen, Germany, 82% of 92 benzidine-exposed chemical workers with bladder cancer were of the slow acetylator phenotype, whereas only 48% of 331 chemical workers who had worked at that plant were of the slow acetylator phenotype. This is in line with several smaller studies, which also show an over-representation of the slow acetylator status in formerly arylamine-exposed subjects with bladder cancer. Some of these studies included also subjects that were exposed to aromatic amines by having applied dyes, paints and varnishes. These European findings are in contrast to a large study on Chinese workers occupationally exposed to aromatic amines. In this study, only five of 38 bladder cancer cases occupationally exposed to arylamines were of the slow acetylator genotype. This is much lower than the ratio of slow acetylators to the general population in China. This points to different mechanisms of susceptibility for bladder cancer upon exposure to aromatic amines between European (Caucasian) and Chinese populations. [Copyright &y& Elsevier] more...
- Published
- 2002
- Full Text
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