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A large odontometric reference database is essential for a clear and reliable differentiation between the teeth of dogs and wolves found in archaeological contexts. However, the data compilation of skeletal material is a slow process because access to dog remains may be difficult. The expansion of a dental database could benefit from computed tomography (CT) scans performed on live dogs during medical appointments and archived in veterinary clinics. To enable it, however, the reliability and accuracy of digital measurements must first be validated. This is the main objective of this paper. We tested the accuracy of dental measurements obtained from CT scans of deceased modern dogs and one Mesolithic dog. CT measurements were obtained using multi-planar reconstruction (MPR) (bi-dimensional images) and three-dimensional volume rendering (3D VR), either using bone or soft tissue windows. Then, measurements were compared with data obtained with a conventional caliper (Fischer Darex®, France). The sample comprised 25 maxillary fourth premolars and 17 maxillary canines. Measurements included the mesiodistal length (MDL) and the vestibular palatine width (VPW). Intra- and inter-observer variations were assessed by calculating the technical error of measurement (TEM). This approach was also used to assess the level of agreement between both techniques, complemented by a Bland-Altman analysis. Intra-observer (TEM = 0.52% to 5.98%) and inter-observer variations (TEM = 3.15% to 7.14%) were relatively small for most standard measurements, windows, and CT approaches. In the case of the MPR approach, the soft tissue window provided less observer variation on the fourth premolar; the same occurred for the bone window regarding the canine. The soft tissue window generally provided less observer variation with the VR approach. CT and caliper agreement ranged from 2.89% to 7.31% (MPR), and from 2.92% to 9.42% (3D VR). At least 91% of the CT measurements were within the Bland-Altman 95% confidence interval. We were able to carry out a thorough odontometric study of the Muge dog skull – a specimen dated to the Mesolithic period, using the MPR approach (with both bone and soft tissue windows). Both the MPR and VR CT scan approaches were validated, thus confirming digital data as an alternative way to generate more comprehensive odontometric references.

Domesticated dogs have been present in the Iberian Peninsula long before other domesticated species, back to the late Palaeolithic period. Their origin is still uncertain, but dogs were already well established during the Chalcolithic period (ca. 5000–4000 BP). This study employed a multidisciplinary approach comprising osteometric, radiographic and palaeogenomic analyses to characterize Chalcolithic Iberian Canis remains. Two Chalcolithic archaeological sites – Leceia, Oeiras, in Portugal, and El Casetón de la Era, Villalba de los Alcores, Valladolid, in Spain – were the main focus of this study. Osteometric and odontometric data from eleven other sites in Iberia were also included. Osteometric results show signs of phenotypic variability, likely the result of human-driven selective pressure. Dental radiographic and dental wear analyses allowed age at death estimation for four individuals (two juvenile and two adults). Three Chalcolithic Iberian dogs had their mitogenomes resequenced and the mitochondrial DNA analysis allowed to assign each individual to two of the major known haplogroups – A and C. Molecular sex infered by the chromosomeX/chromosome1 coverage ratio allowed to identify one female and two males. This study unveils some aspects of the Iberian Chalcolithic dogs: these dogs already exhibited various morphotypes whose profiles might be associated to the performance of certain tasks, as well as mitogenomes of two distinct lineages that help tracking the evolutionary paths of Iberian dogs., The research leading to these results has received funding from FEDER Funds through the Operational Competitiveness Factors Program “COMPETE”, and by National Funds through the Foundation for Science and Technology (FCT Grant POCI-01-0145-FEDER-029545 – PTDC/HAR-ARQ/29545/2017), by the project UIDB/CVT/00772/2020 and associated laboratory AL4AnimalS, as well as from individual grants and contracts: DL57/2016/CP1440/CT0029 (A.E. Pires), DL57/2016/CP1375/CT0003 (I.R. Amorim), 2020.02754.CEECIND (C. Ginja), CEECINS/00127/2018 (J. Requicha), DL 57/2016/CP1443/CT0029 (C. Detry), 2020.06605.BD (A.B. Santos) and 2020.05503.BD (C. Delicado). A considerable amount of the osteometric data included in this research were obtained during the development of the Master thesis and PhD of A. Daza-Perea at Universidad Autónoma de Madrid, thanks to the granting of materials and expertise from C. Liesau, P. Ríos, Argea Consultores S.L., and Trébede, Patrimonio y Cultura S.L. We must thank the support of the FPI PhD BES-2012-056461 grant, which was associated with the HAR2011-28731 Project, both financed by the Spanish Ministry of Economy and Competitiveness.

We investigated the genetic, composition of six Canis remains from western Iberia, directly radiocarbon dated to 7,903-7,570 years (cal BP). They were identified as dogs via their qrchaeological and depositional context, osteometry, and a high percentage of aquatic diet shared with humans. For comparison, genetic data were obtained from an additional 37 Iberian dog remains from the Neolithic to Late Antiquity, as well as two Palaeolithic anda a Chalcolithic Canis identified as wolves. Previous data indicated that dog mtDNA haplogroup A (HgA) is prevalent in extant European dogs (>50%), in the Near East and Asia, but rare or absent (

The study of bioarchaeological evidence associated with burials is essential for achieving a global perspective on cremation as a funerary practice, its chronological and geographical distribution, as well as its inner socio-cultural and technological diversity. However, for that purpose, similar and consistent analyses must be adopted by bioarchaeologists to enable intra- and inter-sites comparisons. The 1995–2015 literature encompassing 84 geographically representative articles concerning bioarchaeological studies of burned human skeletal remains is reviewed herein. The objective was to assess methodological variability. Information concerning colour, fragmentation, skeleton completeness, ‘skeletal region’ representation, non-human funerary assemblage, pre-burning condition of the remains, minimum number of individuals, biological profile, trauma and pathologies was considered. The results demonstrate that certain methods were used by almost all researchers. That was the case for colour description (91 %), skeleton completeness (91 %), minimum number of individuals (96 %), age-at-death (100 %) and sex of the individuals (95 %). Researchers are much more divided about the implementation of the remaining methods. Methodological choices also vary. The asymmetries in the selection of the analyses that are undertaken can lead to different interpretations and conclusions of the contexts under study. This may prevent consistent comparisons within the same site and between different sites. We emphasize the need for bioarchaeologists to discuss and standardize analytical procedures for studying cremated remains.

In the course of a zooarchaeological survey of Holocene sites in southern Portugal, a substantial size increase of cattle bones was noted following the Christian reconquista of the 11the13th centuries AD. A size increase in the course of time within a lineage of domestic livestock is usually considered to represent animal improvement. However several other factors including sex may influence the average size of a sample of mammal bones e cattle exhibit considerable sexual size dimorphism, with bulls being larger than cows. A histogram of the distal widths of a large (n ¼ 44) sample of cattle metacarpals from 15th century Beja (Alentejo, Portugal), revealed a bimodal distribution. It was assumed that the large measurements belonged to males and the small to females. In order to rule out the possibility of a post- Moslem change in the sex ratio of cattle, a sub-sample of 21 cattle metacarpals from Beja was selected and we used genetic markers to identify the sex of the animals to which these metacarpals belonged. The ancient DNA sex of all specimens agreed with the previously assumed sex as determined osteometrically. We conclude that the two nearly separated peaks for the metacarpal distal width measurements do indeed indicate sex. A similar bimodal distribution was obtained from another large but earlier sample of cattle metacarpals from Moslem Alcáçova de Santarém (9the12th century AD). Although these have not been molecularly sexed and since osteometric sexing has now been validated, we conclude that both small (female) and large (male) peaks are smaller than the 15th century ones and that there was an overall size increase or improvement of cattle in this region. Why the Christians improved cattle is unclear, but a selection for larger beeves for meat is one possibility as is the selection of more robust cattle for power. The spread of the quadrangular or chariot plough in Iberia is known to have occurred at this time. We then use the genetically sexed metacarpals to determine which measurements provide reasonable distinction between the sexes. Both the distal width (BFd; as already noted by Svensson et al., 2008; in Swedish medieval cattle) and the width of the lateral condyle (WCL) offer the best distinction. We also used them as a reference ‘collection’ to sex the medieval and post-medieval cattle metacarpals from Launceston Castle in England. This re-visit of the Launceston data corroborates other evidence indicating increased specialisation (milk and veal) in post-medieval cattle husbandry in England.