1. Identification and characterization of 43 novel polymorphic EST-SSR markers for arum lily, Zantedeschia aethiopica (Araceae)
- Author
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Hua-Li Zhang, Lai-Bo Luo, Min Xiong, Xian Wang, Di Zhou, and Zun-Zheng Wei
- Subjects
Expressed Sequence Tags ,Genetic Markers ,Expressed sequence tag ,Zantedeschia ,Polymorphism, Genetic ,biology ,DNA, Plant ,food and beverages ,Locus (genetics) ,Plant Science ,Sequence Analysis, DNA ,biology.organism_classification ,Polymerase Chain Reaction ,Arum ,Araceae ,Loss of heterozygosity ,Genetic variation ,Botany ,Genetics ,Zantedeschia aethiopica ,Microsatellite ,Ecology, Evolution, Behavior and Systematics ,DNA Primers ,Microsatellite Repeats - Abstract
UNLABELLED PREMISE OF THE STUDY A new set of microsatellite or simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) was developed for arum lily (Zantedeschia aethiopica), which is one of the most iconic and widely recognized ornamental plants in the world. • METHODS AND RESULTS Using 2175 unigenes derived from 4283 random ESTs in arum lily, 166 primer pairs were designed and tested for amplification in 24 accessions from Asia, Europe, and Africa. A total of 43 loci were polymorphic, with the number of alleles per locus ranging from two to 10. The observed heterozygosity, expected heterozygosity, and polymorphism information content ranged from 0.2313 to 0.8480, 0.3034 to 0.8648, and 0.1015 to 0.7364, respectively. • CONCLUSIONS These novel polymorphic EST-SSR markers will facilitate future studies of genetic variation and molecular-assisted breeding systems in arum lily.
- Published
- 2012