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1. Solid-phase synthesis of 2'-deoxy-2'-fluoro- beta-D-oligoarabinonucleotides (2'F-ANA) and their phosphorothioate derivatives.

Author

Viazovkina E, Mangos MM, Elzagheid MI, and Damha MJ

Subjects
Arabinose analogs & derivatives, Arabinonucleotides chemical synthesis, Oligonucleotides chemical synthesis, Phosphorothioate Oligonucleotides chemical synthesis
Abstract

This unit describes the chemical synthesis of 2'-deoxy-2'-fluoro-b-D-oligoarabinonucleotides (2'F-ANA), both with phosphodiester and phosphorothioate linkages. The protocols described herein include araF phosphoramidite preparation, assembly on DNA synthesizers, and final deprotection and purification of oligonucleotides.

Published
2002
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