1. Cloning and functional analysis of expansin TaEXPA9 orthologs in winter wheat in frigid regions.
- Author
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ZHAO, Z. Y., HU, B. Z., FENG, X., LI, F. L., HE, F. M., WU, J. W., XU, C. J., LI, L., and XU, Y. Q.
- Subjects
MOLECULAR cloning ,FUNCTIONAL analysis ,AGRICULTURE ,LIPID peroxidation (Biology) ,WHEAT ,WINTER wheat ,ONIONS - Abstract
Long-term low temperatures restrict the regrowth of winter wheat (Triticum aestivum L.), thus decreasing agricultural output. Non-enzymatic expansins, which are related to plant growth, have been reported to respond to drought, salinity, and low-temperature stress. We obtained an expansin 3 gene, TaEXPA9. It is located in winter wheat cv. Dongnong with high cold hardiness. We analyzed the expression patterns of TaEXPA9-A/B/D in this cultivar and conducted a subcellular localization analysis of TaEXPA9-A/B/D in the onion epidermis. Transgenic Arabidopsis thaliana line with EXPA9-A/B/D overexpression was obtained to examine the effects of the orthologous genes of these expansins on plant growth and low-temperature stress resistance. The results showed that EXPA9-A/B/D expression significantly increased at 4 °C, it was higher in the roots than in shoots, and EXPA9-A/B/D was localized in the cell wall. The roots were welldeveloped in the transgenic A. thaliana, and the growth-related markers and setting rate were better than in the wildtype. Recovery was stronger in the transgenic plants after freezing stress. At low-temperature stress, the antioxidant enzyme activities and content of osmoregulatory substances in the TaEXPA9-A/B/D-overexpressing A. thaliana plants were significantly higher than in the wild-type plants, and the degree of membrane lipid peroxidation was lower. In summary, TaEXPA9 orthologous genes participate in the low-temperature stress response, and they might be of great importance in molecular breeding. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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