1. Possible role of the Nt-4/1 protein in macromolecular transport in vascular tissue.
- Author
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Solovyev AG, Makarova SS, Remizowa MV, Lim HS, Hammond J, Owens RA, Kopertekh L, Schiemann J, and Morozov SY
- Subjects
- Arabidopsis Proteins genetics, Biological Transport genetics, Biological Transport physiology, Gene Expression Regulation, Plant, Plant Proteins genetics, Plant Proteins metabolism, Plants, Genetically Modified genetics, Promoter Regions, Genetic, Nicotiana genetics, Arabidopsis Proteins metabolism, Plants, Genetically Modified metabolism, Nicotiana metabolism
- Abstract
The Arabidopsis thaliana 4/1 (At-4/1) protein has a highly α-helical structure with potential to interact both with itself and other protein ligands, including the movement proteins of some plant viruses; the Nicotiana tabacum ortholog (Nt-4/1) has similar structure. Here we describe localization of GUS expression in transgenic N. tabacum seedlings under control of the Nt-4/1 promoter, which indicates that transcription is associated with the veins at certain developmental stages, and especially in the hypocotyl. Viroid accumulation and movement was altered in plants in which 4/1 expression was reduced by virus-induced gene silencing. These localization studies support a role of 4/1 in signaling in the vasculature,including mobility of pathogen-related and cellular RNAs.
- Published
- 2013
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