1. The transcriptomic response to copper exposure in the digestive gland of Japanese scallops (Mizuhopecten yessoensis).
- Author
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Meng, Xiaolin, Tian, Xue, Nie, Guoxing, Wang, Junli, Liu, Mei, Jiang, Keyong, Wang, Baojie, Guo, Qianqian, Huang, Jianrong, and Wang, Lei
- Subjects
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SCALLOPS , *IMMUNE response in fishes , *EXTRACELLULAR matrix , *TOLL-like receptors , *BIOMARKERS , *AQUACULTURE ,FISH digestive organs - Abstract
The present study was conducted to elucidate the effects of copper exposure on the immune system and lipid metabolism of the Japanese scallop, Mizuhopecten yessoensis . Transcriptional levels of differentially expressed genes (DEGs)in M. yessoensis digestive gland tissue were analyzed using the deep-sequencing platform Illumina HiSeq™ 2000. In total, 841 and 877 genes were identified as significantly up- or down-regulated, respectively. In addition, significant enrichment analysis identified 3 gene ontology terms and 15 pathways involved in the response to copper exposure. Analysis of transcripts related to the immune response revealed a complex pattern of innate recognition receptors, including toll-like receptors, NOD-like receptors and downstream pathway effectors, including those involved in apoptosis. Furthermore, genomic analysis revealed that genes involved in extracellular matrix (ECM)-receptor interactions were enriched in Cu-exposed scallop glands. These results will provide a resource for subsequent gene expression studies regarding heavy metal exposure and the identification of copper-sensitive biomarkers for the aquaculture of M. yessoensis . [ABSTRACT FROM AUTHOR]
- Published
- 2015
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