1. Preparation, characterization, and in vitro tumor-suppressive effect of anti-miR-21-equipped RNA nanoparticles.
- Author
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Zhang, Tinghong, Wu, Yunlong, Yang, Dejun, Wu, Cunzao, and Li, Huaqiong
- Subjects
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TRIPLE-negative breast cancer , *EPIDERMAL growth factor receptors , *RNA , *METASTATIC breast cancer , *GENETIC regulation , *APTAMERS , *ANTHRACYCLINES - Abstract
MicroRNAs play an irreplaceable role in gene expression regulation. Upregulation of several miRNAs increases the risk of invasion and metastasis of breast cancer cells. An oncogenic miRNA, miR-21, is highly expressed in triple-negative breast cancer (TNBC) and is associated with tumor proliferation, invasion, carcinogenesis, prognosis, and therapeutic resistance. However, targeted delivery of therapeutic anti-miRNAs into cancer cells remains challenging, especially for TNBC. In this study, we report the application of an RNA nanotechnology-based platform for the targeted delivery of anti-miR-21 by epidermal growth factor receptor (EGFR) aptamer in vitro to TNBC and chemical-resistant breast cancer cells. RNA nanoparticles reduced cell viability and sensitized breast cancer cells to doxorubicin (DOX) treatment in vitro. Inhibition of miR-21 by RNA nanoparticles suppressed TNBC cell invasion, migration, and colony formation. The results indicate the potential application of nanotechnology-based delivery platforms in clinical anti-cancer therapeutics. • A multitude of clinical data revealed the important role of miR-21 in breast cancer. • RNA nanoparticles carrying anit-miR-21 were successfully constructed in the study. • The efficient cellular binding and uptake of 3WJ-EGFRapt/anti-miR-21 NPs were tested. • 3WJ-EGFRapt/anti-miR-21 NPs suppressed cancer cell invasion, migration, and colony formation. • The remarkable synergistic effect of miR-21 knockdown with chemotherapy was revealed. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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