9 results on '"Zeng, Sheng"'
Search Results
2. The phosphoinositide phosphatase MTM-1 regulates apoptotic cell corpse clearance through CED-5-CED-12 in C. elegans.
- Author
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Neukomm LJ, Nicot AS, Kinchen JM, Almendinger J, Pinto SM, Zeng S, Doukoumetzidis K, Tronchère H, Payrastre B, Laporte JF, and Hengartner MO
- Subjects
- Animals, Animals, Genetically Modified, Apoptosis genetics, Apoptosis Regulatory Proteins, Caenorhabditis elegans genetics, Caenorhabditis elegans growth & development, Caenorhabditis elegans Proteins genetics, Carrier Proteins genetics, Cytoskeletal Proteins genetics, Gene Expression Regulation, Developmental, Genes, Helminth, Humans, Membrane Proteins genetics, Models, Biological, Mutation, Phosphoproteins genetics, Phosphoproteins metabolism, Protein Tyrosine Phosphatases, Non-Receptor deficiency, Protein Tyrosine Phosphatases, Non-Receptor genetics, Signal Transduction, rac GTP-Binding Proteins genetics, rac GTP-Binding Proteins metabolism, Apoptosis physiology, Caenorhabditis elegans cytology, Caenorhabditis elegans metabolism, Caenorhabditis elegans Proteins metabolism, Carrier Proteins metabolism, Cytoskeletal Proteins metabolism, Membrane Proteins metabolism, Protein Tyrosine Phosphatases, Non-Receptor metabolism
- Abstract
Multicellular organisms use programmed cell death to eliminate unwanted or potentially harmful cells. Improper cell corpse removal can lead to autoimmune diseases. The development of interventional therapies that increase engulfment activity could represent an attractive approach to treat such diseases. Here, we describe mtm-1, the Caenorhabditis elegans homolog of human myotubularin 1, as a potential negative regulator of apoptotic cell corpse clearance. Loss of mtm-1 function leads to substantially reduced numbers of persistent cell corpses in engulfment mutants, which is a result of a restoration of engulfment function rather than of impaired or delayed programmed cell death. Epistatic analyses place mtm-1 upstream of the ternary GEF complex, which consists of ced-2, ced-5 and ced-12, and parallel to mig-2. Over-activation of engulfment results in the removal of viable cells that have been brought to the verge of death under limiting caspase activity. In addition, mtm-1 also promotes phagosome maturation in the hermaphrodite gonad, potentially through CED-1 receptor recycling. Finally, we show that the CED-12 PH domain can bind to PtdIns(3,5)P(2) (one target of MTM-1 phosphatase activity), suggesting that MTM-1 might regulate CED-12 recruitment to the plasma membrane.
- Published
- 2011
- Full Text
- View/download PDF
3. Holliday junction‑recognition protein modulates apoptosis, cell cycle arrest and reactive oxygen species stress in human renal cell carcinoma
- Author
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Jiang‑Shui Yuan, Zeng‑Sheng Chen, Ke Wang, and Zong‑Liang Zhang
- Subjects
Adult ,Male ,0301 basic medicine ,Cancer Research ,Cell type ,Cell cycle checkpoint ,Cell Survival ,Cell ,Down-Regulation ,Apoptosis ,urologic and male genital diseases ,Resting Phase, Cell Cycle ,Flow cytometry ,03 medical and health sciences ,0302 clinical medicine ,Cell Line, Tumor ,medicine ,Humans ,Carcinoma, Renal Cell ,Aged ,Cell Proliferation ,medicine.diagnostic_test ,Chemistry ,General Medicine ,Middle Aged ,Cell cycle ,G1 Phase Cell Cycle Checkpoints ,Kidney Neoplasms ,female genital diseases and pregnancy complications ,Up-Regulation ,DNA-Binding Proteins ,Oxidative Stress ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Cell culture ,030220 oncology & carcinogenesis ,Cancer research ,Female ,Reactive Oxygen Species ,Holliday junction recognition protein - Abstract
Holliday junction recognition protein (HJURP) is involved in the regulation of mortality in various cell types, including renal cell carcinoma (RCC) cells. The specific mechanisms by which HJURP regulates RCC cell apoptosis and the cell cycle have not been previously investigated, to the best of our knowledge. In the present study, the expression of HJURP in RCC tissues and adjacent paracancerous renal tissue, as well as in RCC cell lines, was analyzed using reverse transcription‑quantitative PCR and western blot analysis. The A498 RCC cells were transfected with an HJURP overexpression vector, which resulted in reduced proliferation, as demonstrated using immunofluorescence staining, a Cell Counting Kit‑8 assay and a colony formation assay. Flow cytometry and terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick end labelling assays were used to determine the effect of HJURP on the cell cycle and apoptosis of RCC cells. Proteins associated with the reactive oxygen species (ROS) status were analyzed using western blot analysis. The expression of HJURP was lower in RCC tissues and cells compared with that in the adjacent paracancerous renal tissues and control cells. Furthermore, overexpression of HJURP resulted in a decrease in cell viability and proliferation in vitro. Overexpression of HJURP resulted in cell cycle arrest at the G0/G1 phase, cell apoptosis and an increase in ROS stress. In addition, the phosphorylated/total sirtuin 1 (SIRT1) protein ratio was decreased, whereas the expression of peroxisome proliferator‑activated receptor (PPAR)γ was increased in the HJURP‑overexpressing RCC cells. In clinical practice, decreased HJURP expression may be associated with poor prognosis in patients with RCC. These results suggest that HJURP may regulate cell apoptosis and proliferation in RCC cells and this may be mediated by PPARγ/SIRT1. Thus, HJURP may be used as a predictor of prognosis in patients with RCC.
- Published
- 2020
4. Growth Suppression Effects of Recombinant Adenovirus Expressing Human Lactoferrin on Cervical CancerIn VitroandIn Vivo
- Author
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Hai Yang, Zhong Liang Jiang, Jian Li, Zeng Sheng Han, Qing Wang Li, Wen Ye Li, and Xiao Bo Zhang
- Subjects
Cancer Research ,Genetic enhancement ,Mice, Nude ,Uterine Cervical Neoplasms ,Apoptosis ,Cell Growth Processes ,Biology ,Adenoviridae ,Mice ,In vivo ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Radiology, Nuclear Medicine and imaging ,Pharmacology ,Lactoferrin ,HEK 293 cells ,Cancer ,Genetic Therapy ,General Medicine ,medicine.disease ,Molecular biology ,In vitro ,HEK293 Cells ,Oncology ,Cell culture ,biology.protein ,Female ,HeLa Cells - Abstract
Human lactoferrin (hLF) is a multifunctional glycoprotein that can inhibit cancer growth. The molecular mechanism of hLF-induced tumor growth inhibition is incompletely understood. Moreover, the adenovirus vector-mediated hLF (Ad-hLF) gene therapy on cervical cancer has not been yet characterized. In this study, the replication-deficient Ad-hLF was used to explore tumor growth suppression effects on cervical cancer in vitro and in vivo. The results showed that the recombinant adenovirus encoding hLF delivery resulted in a more differential tumor growth inhibition, and this growth arrest was caused by cell cycle inhibition at G2/M phase. In addition, Fas, a death-inducing receptor, and Bax, a member of pro-apoptotic Bcl-2 family, were increased in the sample of cervical cancer tissue treated by Ad-hLF. Further, it was also observed that caspase-3 was activated and the expression of anti-apoptotic Bcl-2 was decreased. These results indicated that the growth inhibitory effects of Ad-hLF on cervical cancer were caused by elevated expression of Fas and decreased the ratio of anti- to pro-apoptotic molecule Bcl-2/Bax.
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- 2011
5. Antitumor effects of total alkaloids isolated from Solanum nigrum in vitro and in vivo
- Author
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Jian, Li, Qing-Wang, Li, Da-Wei, Gao, Zeng-Sheng, Han, and Kun, Li
- Subjects
Mice, Inbred BALB C ,Apoptosis ,DNA Fragmentation ,Genes, p53 ,Antineoplastic Agents, Phytogenic ,Immunohistochemistry ,Genes, bcl-2 ,Mice ,Alkaloids ,In Situ Nick-End Labeling ,Animals ,Humans ,Female ,Lymphocytes ,Cell Proliferation ,HeLa Cells ,Solanum nigrum ,bcl-2-Associated X Protein - Abstract
This study demonstrated that the total alkaloids isolated from the traditional Chinese medicinal herb Solanum nigrum Linne (SNL-A) inhibited the growth of human cervical cancer HeLa cells in culture medium with much lower toxicity to human normal lymphocytes. By means of HE staining and TUNEL assay, our results further revealed that SNL-A induced cell death by apoptosis. An immunohistochemical assay showed down-regulation of the bcl-2 and p53 genes and no obvious change of bax gene in the SNL-A treated cells. Subcutaneous injection of HeLa cells induced tumor formation in nude mice, and SNL-A showed a significant inhibitory effect on tumor formation. These results suggested that SNL-A may be a potential, natural apoptosis-inducing agent for cervical cancer.
- Published
- 2008
6. Infection with respiratory syncytial virus influences FasL-mediated apoptosis of pulmonary γδ T cells in a murine model of allergen sensitization.
- Author
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Zeng, Sheng, Wu, Jianqi, Liu, Jing, Qi, Feifei, Kimura, Yoshinobu, Cao, Yaming, and Liu, Beixing
- Subjects
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RESPIRATORY syncytial virus , *APOPTOSIS , *T cells , *ALLERGENS , *SENSITIZATION (Neuropsychology) , *EOSINOPHILS , *LABORATORY mice - Abstract
Background: It has been reported that adoptive transfer of γδ T cells increases the cellular infiltration, especially eosinophils, in the lungs of allergic mice, suggesting that γδ T cells may play a proinflammatory role in allergic airway inflammation. Respiratory syncytial virus (RSV) infection can decrease the number of Th2-type γδ T cells. However, the underlying mechanisms remain unknown. Methods: BALB/c mice were inoculated intranasally with RSV before or after sensitization to OVA. The amounts of Th1/Th2 cytokines as well as the levels of specific antibodies were determined by ELISA. The apoptotic death of pulmonary γδ T cells was analyzed by flow cytometry. Results: Adoptive transfer of γδ T cells increased the production of Th2 cytokines in the lungs and allergy-related antibodies in the serum, further confirming that γδ T cells act as pro-inflammatory cells or a promoter for the development of allergic asthma. RSV infection before sensitization to OVA enhanced apoptotic death of pulmonary γδ T cells. The percentage and absolute number of FasL-expressing γδ T cells in the lungs of allergic mice were elicited significantly by prior RSV infection. Blocking FasL with monoclonal antibody diminished apoptotic death of γδ T cells, suggesting that FasL is important for RSV-induced apoptosis of pulmonary γδ T cells. Conclusions: This work provides evidence that RSV infection suppresses the subsequent development of OVA-induced allergic responses partly by enhancing FasL-mediated apoptosis of pulmonary γδ T cells. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
7. Antitumor activity of polysaccharides isolated from Patrinia heterophylla.
- Author
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Lu, Wen-Zong, Geng, Guo-Xia, Li, Qing-Wang, Li, Jian, Liu, Fu-Zhu, and Han, Zeng-Sheng
- Subjects
ANTINEOPLASTIC agents ,VALERIANACEAE ,PATRINIA ,CANCER treatment ,POLYSACCHARIDES ,IMMUNOCHEMISTRY ,LABORATORY mice - Abstract
The research investigated the effect of Patrinia heterophylla Bunge (Valerianaceae) polysaccharides (PHB-P1) on U14-bearing mice. The tumor weight of mice treated with PHB-P1 (30, 60 mg/kg body weight) was significantly lower than that of the control group, a decrease of serum lactate dehydrogenase (LDH) activity was observed, and the serum alkaline phosphatase (AKP) level was increased slightly. The number of apoptotic tumor cells was significantly increased in the mice by treatment of PHB-P1 (30, 60 mg/kgbw). Cell cycle analysis showed the accumulation of tumor cells in the G2/M phase and a relative decrease of the S phase. By the immunohistochemical analysis, PHB-P1 (30, 60 mg/kgbw) might up-regulate the expression of p53 and Bax, and significantly inhibited the expression of Bcl-2 in tumor tissues. In conclusion, PHB-P1 could inhibit tumor growth and induce tumor cell apoptosis. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
8. Anti-Tumor Activity of Polysaccharides Isolated from Patrinia scabra Bunge on U14 Cervical Carcinoma Bearing Mice.
- Author
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Wen-Zong Lu, Guo-Xia Geng, Qing-Wang Li, Jian Li, Fu-Zhu Liu, Zeng-Sheng Han, Da-Wei Gao, Xin Yan, and Xiao-Lei Yang
- Subjects
CERVICAL cancer ,CANCER cells ,CANCER treatment ,POLYSACCHARIDES ,LABORATORY mice ,REVERSE transcriptase polymerase chain reaction ,THERAPEUTICS - Abstract
The aim of our study was to investigate the effect of Patrinia scabra Bunge polysaccharide (PSB-P2) on cervical cancer cell (U14)-bearing mice. The tumor weight of mice treated with PSB-P2 (40, 80 mg/kg b.w.) was significantly lower than that of the control group and serum lactate dehydrogenase (LDH) activity was decreased, while serum alkaline phosphatase (AKP) level was only changed slightly. Meanwhile, the number of apoptotic tumor cells was significantly increased in the mice by the treatment of PSB-P2 (40, 80 mg/kg b.w.). At the same time, cell cycle analysis showed the accumulation of tumor cells in the G0/G1 phase and a relative decrease in the S phase. On the other hand, using the reverse transcription-polymerase chain reaction (RT-PCR) assay, PSB-P2 (40, 80 mg/kg b.w.) showed the up-regulation of p53 and Bax, and significant inhibition of Bcl-2 in tumor tissues. It suggests a possible mechanism of the inhibitory effect of PSB-P2 on tumor growth. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
9. Melatonin modulates the functions of porcine granulosa cells via its membrane receptor MT2 in vitro.
- Author
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He, Ya-Mei, Deng, Hong-Hui, Shi, Mei-Hong, Bodinga, Bello Musa, Chen, Hua-Li, Han, Zeng-Sheng, Jiang, Zhong-Liang, and Li, Qing-Wang
- Subjects
- *
MELATONIN , *GRANULOSA cells , *CIRCADIAN rhythms , *APOPTOSIS , *PROGESTERONE , *MESSENGER RNA - Abstract
Melatonin ( N -acetyl-5-methoxytryptamine) is documented as a hormone involved in the circadian regulation of physiological and neuroendocrine function in mammals. Herein, the effects of melatonin on the functions of porcine granulosa cells in vitro were investigated. Porcine granulosa cells were cultivated with variable concentrations of melatonin (0, 0.001, 0.01, 0.1, 1.0, and 10 ng/mL) for 48 h. Melatonin receptor agonist (IIK7) and antagonist (Luzindole, 4P-PDOT) were used to further examine the action of melatonin. The results showed optimum cell viability and colony-forming efficiency of porcine granulosa cells at 0.01 ng/mL melatonin for 48-h incubation period. The percentage of apoptotic granulosa cells was significantly reduced by 0.01 and 0.1 ng/mL melatonin within the 48-h incubation period as compared with the rest of the treatments. Estradiol biosynthesis was significantly stimulated by melatonin supplementation and suppressed for the progesterone secretion; the minimum ratio of progesterone to estradiol was 1.82 in 0.01 ng/mL melatonin treatment after 48 h of cultivation. Moreover, the expression of BCL-2, CYP17A1 , CYP19A1 , SOD1 , and GPX4 were up-regulated by 0.01 ng/mL melatonin or combined with IIK7, but decreased for the mRNA levels of BAX , P53 , and CASPASE-3 , as compared with control or groups treated with Luzindole or 4P-PDOT in the presence of melatonin. In conclusion, the study demonstrated that melatonin mediated proliferation, apoptosis, and steroidogenesis in porcine granulosa cells predominantly through the activation of melatonin receptor MT2 in vitro , which provided evidence of the beneficial role of melatonin as well as its functional mechanism in porcine granulosa cells in vitro . [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
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