1. MicroRNA-140 inhibits proliferation and promotes apoptosis and cell cycle arrest of prostate cancer via degrading SOX4.
- Author
-
Liu Y, Li F, Lai D, Xie Q, Yin Y, Yang M, and Su Z
- Subjects
- Gene Expression Regulation, Neoplastic, Humans, Male, Prostatic Neoplasms genetics, Prostatic Neoplasms metabolism, Proteolysis, SOXC Transcription Factors genetics, Tumor Cells, Cultured, Apoptosis, Cell Cycle Checkpoints, Cell Proliferation, MicroRNAs genetics, Prostatic Neoplasms pathology, SOXC Transcription Factors metabolism
- Abstract
Purpose: To explore the regulatory roles of microRNA-140 and SOX4 in prostate cancer (PCa) tissues and paracancerous tissues, and their underlying mechanism., Methods: MicroRNA-140 expressions in PCa tissues, paracancerous tissues and PCa cell lines were detected by quantitative real-time polymerase chain reaction (qRT-PCR). Proliferation, apoptosis and cell cycle of PCa cells after altering expressions of microRNA-140 and SOX4 were detected by MTT assay and flow cytometry, respectively. The regulatory effect of microRNA-140 on SOX4 was detected by Western blot and qRT-PCR. The binding condition of microRNA-140 on SOX4 was verified by luciferase reporter gene assay., Results: MicroRNA-140 was downregulated in PCa tissues compared to paracancerous tissues. In particular, lower expression of microRNA-140 was found in PCa with Grade I+II compared to Grade III+IV. In vitro, microRNA-140 expression was negatively correlated with proliferative and invasive abilities, while positively correlated with apoptosis of PCa cells. MicroRNA-140 promoted cell cycle arrest in G0/G1 phase. SOX4 expression was inhibited by microRNA-140 overexpression in PCa cells., Conclusions: Downregulated microRNA-140 promotes proliferation and cell cycle arrest, but inhibits apoptosis of PCa cells. MicroRNA-140 inhibits PCa development via degrading SOX4.
- Published
- 2019