1. miR-31-5p promotes proliferation and inhibits apoptosis of goat hair follicle stem cells by targeting RASA1/MAP3K1 pathway.
- Author
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Feng Y, Wang J, Ma J, Zhang L, Chu C, Hu H, Wang Y, and Li Y
- Subjects
- Animals, Cell Proliferation, Cells, Cultured, Goats, Apoptosis, Hair Follicle metabolism, MAP Kinase Kinase Kinase 1 metabolism, MicroRNAs metabolism, Stem Cells metabolism, p120 GTPase Activating Protein metabolism
- Abstract
The Yangtze River Delta white goat is a sole goat species that can naturally produce superior-quality brush hair. It's worth to mention that study the developmental mechanism of goat hair follicle stem cells is vital for future breed preservation and molecular breeding. In this study, we successfully isolated hair follicle stem cells from the skin tissue of fetal sheep neck spine, and harvested superior-quality and normal-quality brush hair goat tissue. The expression of miR-31-5p in goat hair follicle stem cells was verified by qPCR and Western blot. The effects of overexpression or inhibition of miR-31-5p on the proliferation and apoptosis of hair follicle stem cells were detected by EdU, CCK-8, flow cytometry, etc. miR-31-5p can significantly improve cell proliferation and inhibit cell apoptosis by targeting RASA1 and upregulating MAP3K1 level, whereas miR-31-5p knockdown led to an opposite effect. These results reveal a miR-31-5p-associated regulatory network between miR-31-5p and RASA1/MAP3K1 during the progression of superiorquality brush hair traits., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2021
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