1. Expression of the apoptotic genes Bax and Bcl-2 in rat hippocampus following transient ischemia.
- Author
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Majidy-Zolbin, M., Rezaei, L., Azami-Tameh, A., Rezvani, Z., Atlasi, M. A., Nikzad, H., Naji, T., and Naderian, H.
- Subjects
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HIPPOCAMPUS physiology , *TRANSIENT ischemic attack , *ANIMAL experimentation , *APOPTOSIS , *BIOLOGICAL models , *GENE expression , *POLYMERASE chain reaction , *RATS , *REVERSE transcriptase polymerase chain reaction , *GENETICS - Abstract
Background: Cerebral ischemia is considered as one of the leading causes of death and involved in neuronal apoptosis. This study aimed to evaluate the Bax and Bcl-2 expression in the rat hippocampus after reperfusion following transient ischemia. Materials and Methods: In this experimental study, transient global cerebral ischemia (15 and 30 minutes) was induced in adult male Wistar rats (n=5 in each group) by the occlusion of bilateral common carotid artery followed by reperfusion for 24 and 72 hours. Five Sham-operated animals, without any intervention, considered as a control group. The expression of Bcl-2 and Bax genes were detected by reverse transcription (RT)-PCR in all groups. Results: Transcription and expression of both Bcl-2 and Bax genes were increased after global cerebral ischemia in all experimental groups; the two gene expression increment in the experimental groups (2, 3 and 4) was more detectable than the first group. Conclusion: According to the results, transient cerebral ischemia activates Bcl-2 and Bax apoptotic genes that play an important role in the protection and induction of cell death in the hippocampal neurons, respectively. [ABSTRACT FROM AUTHOR]
- Published
- 2012