1. Effects of Danshen Injection () on inhibiting proliferation and inducing apoptosis through down-regulation of mutant JAK2 gene and its protein phosphorylation in human erythroid leukemic cells.
- Author
-
Li LJ, Xu NW, Gao RL, Lin XJ, Qiu HY, Liu WH, Jin YJ, and Zhao ML
- Subjects
- Base Sequence, DNA Primers, Humans, Janus Kinase 2 genetics, Leukemia, Erythroblastic, Acute enzymology, Leukemia, Erythroblastic, Acute pathology, Phosphorylation, Polymerase Chain Reaction, Apoptosis drug effects, Cell Proliferation drug effects, Down-Regulation drug effects, Janus Kinase 2 metabolism, Leukemia, Erythroblastic, Acute metabolism, Mutation, Plant Extracts pharmacology, Salvia miltiorrhiza chemistry
- Abstract
Objective: To explore the effects of Danshen Injection () on inhibition proliferation, inducing apoptosis and its possible mechanisms on human erythroid leukemic (HEL) cells., Methods: The commercial Chinese patent medicine of Danshen Injection was extracted and isolated from Chinese herb of Salvia miltiorrhiza bung. The inhibition effects of proliferation were assayed by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) method in HEL cells treated by Danshen Injection at various concentrations for 48 h. The cellular apoptosis was observed in morphology, analyzed by flow cytometry with annexin V and propidium iodide (PI) staining, and examined by DNA degradation ladder on agarose gel electrophoresis. Meanwhile, the expression levels of mutant Janus kinasez (JAK2) gene and phosphorylation-JAK2 (P-JAK2) protein were detected by allele specific-polymerase chain reaction and Western blot., Results: The proliferation of HEL cells was effectively inhibited by Danshen Injection in a dose-dependent manner, with suppression rates from 19.46±2.31% to 50.20±5.21%. Typical apoptosis cells was observed in Danshen Injection treated HEL cells, the rates of annexin V positive cells increased obviously in a dose-dependent manner, as well as the DNA degradation ladder of apoptosis revealed on gel electrophoresis. The expression levels of mutant JAK2 gene and P-JAK2 protein reduced gradually with increasing dosage of Danshen injection., Conclusion: Danshen Injection could not only significantly inhibit the proliferation, but also induce apoptosis in HEL cells; down-regulation of the mutant JAK2 gene and P-JAK2 protein expressions are probably one of its molecular mechanisms.
- Published
- 2014
- Full Text
- View/download PDF