1. Sorafenib inhibits p38α activity in colorectal cancer cells and synergizes with the DFG-in inhibitor SB202190 to increase apoptotic response.
- Author
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Grossi V, Liuzzi M, Murzilli S, Martelli N, Napoli A, Ingravallo G, Del Rio A, and Simone C
- Subjects
- Animals, Caspase 3 drug effects, Cell Line, Tumor, Colorectal Neoplasms metabolism, Drug Synergism, Female, Humans, Mice, Mice, Nude, Neoplasm Transplantation, Niacinamide pharmacology, Signal Transduction drug effects, Sorafenib, Transplantation, Heterologous, Apoptosis drug effects, Colorectal Neoplasms drug therapy, Imidazoles pharmacology, Mitogen-Activated Protein Kinase 14 antagonists & inhibitors, Niacinamide analogs & derivatives, Phenylurea Compounds pharmacology, Pyridines pharmacology
- Abstract
In the search for new strategies to efficiently fight colorectal cancer, efforts are being increasingly focused on targeting regulatory signaling pathways involved in cancer-specific features. As a result, several studies have recently addressed the therapeutic potential of molecularly-targeted drugs capable of inhibiting the activity of protein kinases involved in relevant signaling cascades. Here we show that simultaneous inhibition of the DFG-in and DFG-out conformations of p38α by means of type-I and type-II inhibitors is beneficial to impair more efficiently its kinase activity. Moreover, we found that SB202190 (type-I) and sorafenib (type-II) synergize at the molecular and biological level, as co-treatment with these compounds enhances tumor growth inhibition and induction of apoptosis both in colorectal cancer cell lines and animal models. These results support the need to reconsider sorafenib as a therapeutic agent against colorectal cancer and provide new insights that underline the importance to elucidate the activity of protein kinase inhibitors for the treatment of colorectal carcinoma. more...
- Published
- 2012
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