1. Dichloromethane Extract of Fermentation Broth by Co-Culture of Morchella esculenta and Coprinus comatus Induces Apoptosis in U251 Cells via Mitochondrial Intrinsic Pathway
- Author
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Hongkun Xue, Xiaohong Zhao, Jintian Tang, Linyu Jia, Zhong Xue, Hongzhu Yu, Xuan Liu, Xu Cai, Wang Huiguo, Liya Li, Qian Li, Benke Chen, Xianwei Zou, and Hua Wang
- Subjects
Apoptosis ,Morchella esculenta ,Applied Microbiology and Biotechnology ,Flow cytometry ,Coprinus ,Ascomycota ,Cell Line, Tumor ,Drug Discovery ,medicine ,Humans ,Cell Proliferation ,bcl-2-Associated X Protein ,Pharmacology ,Methylene Chloride ,Messenger RNA ,biology ,medicine.diagnostic_test ,Caspase 3 ,Plant Extracts ,Chemistry ,Cell Cycle ,Glioma ,biology.organism_classification ,Molecular biology ,Coculture Techniques ,In vitro ,Mitochondria ,Cell culture ,Fermentation ,Coprinus comatus - Abstract
The current study was designed to investigate the inhibitory effects of dichloromethane extract of fermentation broth by co-culture of Morchella esculenta and Coprinus comatus (DCMM) on human glioma U251 cells in vitro and its possible underlying mechanisms. The proliferation of U251 cells was inhibited by DCMM with different concentrations by the CCK-8 assay. Besides, flow cytometry assay was used to evaluate the DCMM promoted U251 cell apoptosis rate in a dose-dependent manner. DCMM with different concentrations (10 μg·mL-1, 20 μg·mL-1, and 40 μg·mL-1) significantly enhanced the expression of caspases-3 activity after 24 h. In addition, DCMM with different concentrations significantly increased caspase-3 and Bax, and decreased Bcl-2 expressions at both mRNA and protein levels. DCMM can remarkably inhibit the proliferation and promote cell apoptosis of human glioma U251 cells. The possible underlying mechanisms could be related to induction of apoptosis of human glioma U251 cells by mitochondrial intrinsic pathway.
- Published
- 2020
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