1. Wogonin Inhibits Apoptosis and Necroptosis Induced by Nephropathogenic Infectious Bronchitis Virus in Chicken Renal Tubular Epithelial Cells.
- Author
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Qi Q, Li Y, Ding M, Huang C, Omar SM, Shi Y, Liu P, Cai G, Zheng Z, Guo X, and Gao X
- Subjects
- Animals, Membrane Potential, Mitochondrial drug effects, Antiviral Agents pharmacology, Mitochondria drug effects, Mitochondria metabolism, Coronavirus Infections virology, Coronavirus Infections drug therapy, Poultry Diseases virology, Poultry Diseases drug therapy, Virus Replication drug effects, Cells, Cultured, Flavanones pharmacology, Epithelial Cells drug effects, Epithelial Cells virology, Epithelial Cells metabolism, Necroptosis drug effects, Apoptosis drug effects, Kidney Tubules virology, Kidney Tubules drug effects, Kidney Tubules cytology, Kidney Tubules pathology, Chickens
- Abstract
NIBV is an acute and highly contagious virus that has a major impact on the poultry industry. Wogonin, as a flavonoid drug, has antiviral effects, but there have been no reports indicating its role in renal injury caused by NIBV infection. The aim of this study is to investigate the antiviral effect of wogonin against NIBV. Renal tubular epithelial cells were isolated and cultured, and divided into four groups: Con, Con+Wog, NIBV and NIBV+Wog. We found that wogonin significantly inhibited the copy number of NIBV and significantly alleviated NIBV-induced cell apoptosis and necrosis. Moreover, wogonin inhibited the reduction in mitochondrial membrane potential and the aberrant opening of mPTP caused by NIBV. In conclusion, wogonin can protect renal tubular epithelial cells from damage by inhibiting the replication of NIBV and preventing mitochondrial apoptosis and necroptosis induced by NIBV.
- Published
- 2024
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