5 results on '"Groves MJ"'
Search Results
2. Profile of adult rat sensory neuron loss, apoptosis and replacement after sciatic nerve crush.
- Author
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Groves MJ, Schänzer A, Simpson AJ, An SF, Kuo LT, and Scaravilli F
- Subjects
- Animals, Axotomy, Bromodeoxyuridine, Cell Count, Cell Division physiology, Cell Nucleus physiology, Cell Nucleus ultrastructure, Female, Ganglia, Spinal cytology, Male, Nerve Crush, Nerve Degeneration pathology, Neurons, Afferent cytology, Rats, Rats, Sprague-Dawley, Reaction Time physiology, Recovery of Function physiology, Sciatic Neuropathy pathology, Apoptosis physiology, Ganglia, Spinal growth & development, Nerve Degeneration physiopathology, Nerve Regeneration physiology, Neurons, Afferent physiology, Sciatic Neuropathy physiopathology
- Abstract
Following permanent transection of the adult rat sciatic nerve, sensory neuron apoptosis in the contributing L4 and L5 dorsal root ganglia can be observed for at least 6 months afterwards. To establish the profile of any sensory neuron apoptosis and loss over time when axonal regeneration is allowed, serial sections of L4 and L5 ganglia were examined and the neurons counted using a stereological technique 1, 2 and 3 months after crushing the right sciatic nerve at mid-thigh level. Our results show that an identical degree of sensory neuron loss and apoptosis occurs 1 month after crush as at 1 month after permanent transection. However, at 3 months no neurons undergoing apoptosis could be observed and no significant loss could be detected in the ipsilateral ganglia when compared to unoperated controls. One explanation was a neuronal replacement mechanism, which was investigated by administering bromodeoxyuridine to rats for 1 month after sciatic nerve transection or crush, prior to detection using immunohistochemistry on sections of their ganglia after 2 months. The presence of bromodeoxyuridine in the nuclei of occasional cells that would be counted as neurons on the basis of size and morphology indicates that a process of apparent neurogenesis may underlie the profile of sensory neuron loss after axotomy.
- Published
- 2003
- Full Text
- View/download PDF
3. Inhibition of sensory neuron apoptosis and prevention of loss by NT-3 administration following axotomy.
- Author
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Groves MJ, An SF, Giometto B, and Scaravilli F
- Subjects
- Animals, Apoptosis drug effects, Axotomy, Cell Count, Ganglia, Spinal drug effects, Microscopy, Electron, Nerve Growth Factors pharmacology, Neurons, Afferent drug effects, Neurons, Afferent ultrastructure, Neurotrophin 3, Rats, Rats, Sprague-Dawley, Sciatic Nerve ultrastructure, Apoptosis physiology, Axons physiology, Ganglia, Spinal cytology, Nerve Growth Factors physiology, Neurons, Afferent physiology
- Abstract
Following permanent transection of their peripheral axons, a proportion of adult rat dorsal root ganglion neurons undergo programmed cell death (apoptosis) over a period of months. The underlying causes of this neuron loss are unclear, but may involve the interruption of the supply of target-derived neurotrophic factors, the replacement of which could prevent this loss from occurring. To investigate whether the administration of neurotrophic factors can prevent the dorsal root ganglion neuron death in adults, a 1 mg/ml solution of ciliary neurotrophic factor or of NT-3 was applied via a silicon reservoir to the proximal stump after unilateral sciatic transection at mid-thigh level. The incidence of apoptotic neurons and neuronal loss in the L4 and L5 ganglia ipsilateral to sciatic nerve transection when compared with the contralateral ganglia was then measured 1 month later. This was assessed by examining serial sections of ganglia for neurons undergoing apoptosis and expressing the total counted as a percentage of the total number of neurons estimated using a stereological neuron counting technique. Our results show that NT-3 administration significantly reduced the incidence of apoptotic neurons and prevented neuron loss, while CNTF had no effect on either parameter., (Copyright 1999 Academic Press.)
- Published
- 1999
- Full Text
- View/download PDF
4. Axotomy-induced apoptosis in adult rat primary sensory neurons.
- Author
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Groves MJ, Christopherson T, Giometto B, and Scaravilli F
- Subjects
- Animals, Lumbosacral Region, Rats, Apoptosis physiology, Axotomy, Ganglia, Spinal cytology, Neurons, Afferent physiology, Sciatic Nerve physiology
- Abstract
Neuronal death following unilateral axotomy of a sensory nerve has long been inferred from neuronal counts of dorsal root ganglion neurons, using the contralateral ganglia as a control. The counting methods used usually involved the counting of neuronal nucleoli and made assumptions about them which could conceivably be flawed. Very few studies have used direct observations of dying or degenerating neurons to address questions concerning the duration of the period of neuronal death or the mechanisms involved in this process. Here we describe a morphological, morphometric and histochemical study into the nature and duration of sensory neuron death following transection and ligation of the sciatic nerve at mid-thigh level in the adult rat. We show that at least some of this neuronal loss occurs by apoptosis as defined by morphological criteria and in situ end-labelling of damaged DNA. Absolute numbers of apoptotic neurons were counted from serial paraffin sections of ganglia and estimates of neuronal numbers obtained by disector analysis at 1, 2, 3 and 6 months after axotomy. Using this approach we show that axotomy-induced apoptosis begins at around 1 week and continues up to at least 6 months after axotomy.
- Published
- 1997
- Full Text
- View/download PDF
5. Inhibition of sensory neuron apoptosis and prevention of loss by NT-3 administration following axotomy
- Author
-
Francesco Scaravilli, M Groves, Shu Fang An, Bruno Giometto, Groves, Mj, An, Sf, Giometto, B, and Scaravilli, F
- Subjects
medicine.medical_specialty ,medicine.medical_treatment ,Apoptosis ,Cell Count ,Ciliary neurotrophic factor ,Rats, Sprague-Dawley ,Developmental Neuroscience ,Dorsal root ganglion ,Neurotrophin 3 ,Neurotrophic factors ,Internal medicine ,Ganglia, Spinal ,medicine ,Animals ,Nerve Growth Factors ,Neurons, Afferent ,biology ,business.industry ,Axotomy ,Sciatic Nerve ,Sensory neuron ,Axons ,Rats ,Microscopy, Electron ,medicine.anatomical_structure ,Endocrinology ,Nerve growth factor ,nervous system ,Neurology ,biology.protein ,Neuron ,business ,Neuron death ,Neuroscience - Abstract
Following permanent transection of their peripheral axons, a proportion of adult rat dorsal root ganglion neurons undergo programmed cell death (apoptosis) over a period of months. The underlying causes of this neuron loss are unclear, but may involve the interruption of the supply of target-derived neurotrophic factors, the replacement of which could prevent this loss from occurring. To investigate whether the administration of neurotrophic factors can prevent the dorsal root ganglion neuron death in adults, a 1 mg/ml solution of ciliary neurotrophic factor or of NT-3 was applied via a silicon reservoir to the proximal stump after unilateral sciatic transection at mid-thigh level. The incidence of apoptotic neurons and neuronal loss in the L4 and L5 ganglia ipsilateral to sciatic nerve transection when compared with the contralateral ganglia was then measured 1 month later. This was assessed by examining serial sections of ganglia for neurons undergoing apoptosis and expressing the total counted as a percentage of the total number of neurons estimated using a stereological neuron counting technique. Our results show that NT-3 administration significantly reduced the incidence of apoptotic neurons and prevented neuron loss, while CNTF had no effect on either parameter. (C) 1999 Academic Press.
- Published
- 1999
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