1. Methylation‐mediated miR‐214 regulates proliferation and drug sensitivity of renal cell carcinoma cells through targeting LIVIN
- Author
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Xiaogang Chen, Pingcheng Yuan, Ding Wu, Xianlong Li, Yuan Yuan, Qinghan Zhang, Enying Huang, Qianliang Wang, Hao Xu, Yong Liu, Shangjun Wu, Zhan Shi, Qin Ke, Xin Shen, Qing Mao, and Wei Jiang
- Subjects
Male ,0301 basic medicine ,medicine.medical_treatment ,Apoptosis ,LIVIN ,chemotherapy ,urologic and male genital diseases ,Inhibitor of Apoptosis Proteins ,Mice ,0302 clinical medicine ,Renal cell carcinoma ,miR-214 ,media_common ,Mice, Inbred BALB C ,Methylation ,RCC ,Kidney Neoplasms ,female genital diseases and pregnancy complications ,Neoplasm Proteins ,Gene Expression Regulation, Neoplastic ,030220 oncology & carcinogenesis ,Molecular Medicine ,Original Article ,Drug ,media_common.quotation_subject ,Down-Regulation ,Mice, Nude ,Antineoplastic Agents ,Inhibitor of apoptosis ,DNA methyltransferase ,miR‐214 ,Cell Line ,03 medical and health sciences ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Carcinoma, Renal Cell ,neoplasms ,Adaptor Proteins, Signal Transducing ,Cell Proliferation ,Chemotherapy ,business.industry ,DNMT1 ,Original Articles ,Cell Biology ,DNA Methylation ,medicine.disease ,MicroRNAs ,HEK293 Cells ,030104 developmental biology ,Cancer research ,methylation ,business - Abstract
LIVIN, a member of the inhibitor of apoptosis proteins (IAPs), is reported playing important roles in the development and progression of multiple human cancers. However, its underlined mechanisms in human renal cell carcinoma (RCC) are still needed to be clarified. In the present study, we reported that inhibition of miR‐214 promoted the expression of LIVIN, then facilitated RCC cells growth and reduced the sensitivity of RCC cells to chemotherapeutic drugs. In constant, overexpression of miR‐214 had contradictory effects. Further investigation showed that miR‐214 was down‐regulated in RCC because of abnormal methylation. In addition, DNA methyltransferase DNMT1, miR‐214 and LIVIN are directly correlated in RCC patients. In conclusion, these results suggest that abnormal miR‐214 methylation negatively regulates LIVIN, which may promote RCC cells growth and reduced the sensitivity of RCC cells to chemotherapeutic drugs.
- Published
- 2020
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