1. Effects of sulforaphane on the oxidative response, apoptosis, and the transcriptional profile of human stomach mucosa cells in vitro.
- Author
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Santos PW, Machado ART, De Grandis R, Ribeiro DL, Tuttis K, Morselli M, Aissa AF, Pellegrini M, and Antunes LMG
- Subjects
- Anticarcinogenic Agents pharmacology, Antioxidants pharmacology, Cell Line, Cell Survival drug effects, Humans, Mucous Membrane metabolism, NF-E2-Related Factor 2 metabolism, Oxidation-Reduction drug effects, Reactive Oxygen Species metabolism, Signal Transduction drug effects, Sulfoxides, Up-Regulation drug effects, Apoptosis drug effects, Isothiocyanates pharmacology, Mucous Membrane drug effects, Oxidative Stress drug effects, Stomach drug effects, Transcription, Genetic drug effects
- Abstract
Oxidative stress is a critical factor in the pathogenesis of several gastrointestinal diseases. Sulforaphane (SFN), a bioactive compound found in cruciferous vegetables, activates the redox-sensitive nuclear erythroid 2-related factor 2 (NRF2). In addition to its protective role, SFN exerts cytotoxic effects on cancer cells. However, there is a lack of information concerning the toxicity of SFN in normal cells. We investigated the effects of SFN on cell viability, antioxidant defenses, and gene expression in human stomach mucosa cells (MNP01). SFN reduced ROS formation and protected the cells against induced oxidative stress but high concentrations increased apoptosis. An intermediate SFN concentration (8 μM) was chosen for RNA sequencing studies. We observed upregulation of genes of the NRF2 (antioxidant) pathway, the DNA damage response, and apoptosis signaling; whereas SFN downregulated cell cycle and DNA repair pathway genes. SFN may be cytoprotective at low concentrations and cytotoxic at high concentrations., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
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