1. Endoplasmic reticulum in the penumbra following middle cerebral artery occlusion in the rabbit.
- Author
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Huai-jun Liu, Ji-Ping Yang, Cang-Hai Wang, Rui-Chun Liu, Ying Li, and Chun-Yan Li
- Subjects
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ENDOPLASMIC reticulum , *ARTERIAL occlusions , *CEREBRAL artery physiology , *CEREBRAL ischemia , *IMMUNOHISTOCHEMISTRY , *LABORATORY rabbits , *APOPTOSIS , *DIAGNOSIS - Abstract
Caspase-12 has been localized to endoplasmic reticulum (ER) and showed to involve ER stress-induced apoptosis. In the present work we investigated the temporospatial alterations of caspase-12 immunoreactivity in the penumbra following cerebral ischemia/reperfusion in rabbit. Transient cerebral ischemia was produced by intraluminal occlusion of the middle cerebral artery for 2 h followed by 1 h, 6 h, 1 day, 3 days, 7 days and 14 days of reperfusion. Caspase-12 immunohistochemistry was first increased in the penumbra 1 h after reperfusion, with a peak at day 1 to day 3, and then gradually decreased to basal level at day 14. The number of TUNEL-positive cells and ultrastructural observation of brain sections in the penumbra showed a similar change at the same time points. ER mediated by caspase-12 participated in apoptosis induced by cerebral ischemia/reperfusion injury, which may provide a new area for therapeutic intervention to ameliorate outcomes following cerebral ischemia. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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