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Your search keyword '"Makarova, Katerina"' showing total 2 results
Start Over Author "Makarova, Katerina" Topic apocynaceae
2 results on '"Makarova, Katerina"'

1. Geissospermiculatine, a New Alkaloid from Geissospermum reticulatum Bark.

Author

Sajkowska-Kozielewicz JJ, Kozielewicz P, Makarova K, Stocki M, Barnes NM, and Paradowska K

Subjects
Animals, Embryo, Nonmammalian drug effects, Humans, THP-1 Cells, Apocynaceae chemistry, Cytotoxins pharmacology, Embryo, Nonmammalian pathology, Indole Alkaloids pharmacology, Plant Bark chemistry, Plant Extracts pharmacology, Zebrafish growth & development
Abstract

A new alkaloid, geissospermiculatine was characterized in Geissospermum reticulatum A. H. Gentry bark (Apocynaceae). Here, following a simplified isolation protocol, the structure of the alkaloid was elucidated through GC-MS, LC-MS/MS, 1D, and 2D NMR (COSY, ROESY, HSQC, HMBC, 1 H- 15 N HMBC). Cytotoxic properties were evaluated in vitro on malignant THP-1 cells, and the results demonstrated that the cytotoxicity of the alkaloid (30  μg/mL) was comparable with staurosporine (10  μM). Additionally, the toxicity was tested on zebrafish ( Danio rerio ) embryos in vivo by monitoring their development (0-72 h); toxicity was not evident at 30  μg/mL.

Published
2020
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2. Geissospermiculatine, a New Alkaloid from Geissospermum reticulatum Bark

Author

Sajkowska-Kozielewicz, Joanna J., Kozielewicz, Paweł, Makarova, Katerina, Stocki, Marcin, Barnes, Nicholas M., and Paradowska, Katarzyna

Subjects
endocrine system, Danio rerio, Embryo, Nonmammalian, Cytotoxins, Plant Extracts, THP-1 Cells, Communication, Geissospermum reticulatum, alkaloids, complex mixtures, NMR, Indole Alkaloids, Apocynaceae, lcsh:QD241-441, cytotoxic effects, lcsh:Organic chemistry, Plant Bark, Animals, Humans, Zebrafish
Abstract

A new alkaloid, geissospermiculatine was characterized in Geissospermum reticulatum A. H. Gentry bark (Apocynaceae). Here, following a simplified isolation protocol, the structure of the alkaloid was elucidated through GC-MS, LC-MS/MS, 1D, and 2D NMR (COSY, ROESY, HSQC, HMBC, 1H-15N HMBC). Cytotoxic properties were evaluated in vitro on malignant THP-1 cells, and the results demonstrated that the cytotoxicity of the alkaloid (30 μg/mL) was comparable with staurosporine (10 μM). Additionally, the toxicity was tested on zebrafish (Danio rerio) embryos in vivo by monitoring their development (0–72 h); toxicity was not evident at 30 μg/mL.

Published
2021

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