1. Role of proteasome-dependent protein degradation in long-term operant memory in Aplysia.
- Author
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Lyons LC, Gardner JS, Gandour CE, and Krishnan HC
- Subjects
- Analysis of Variance, Animals, Anisomycin pharmacology, Aplysia drug effects, Conditioning, Operant drug effects, Cysteine Proteinase Inhibitors pharmacology, Leupeptins pharmacology, Memory, Long-Term drug effects, Proteasome Endopeptidase Complex drug effects, Time Factors, Aplysia physiology, Conditioning, Operant physiology, Memory, Long-Term physiology, Proteasome Endopeptidase Complex metabolism
- Abstract
We investigated the in vivo role of protein degradation during intermediate (ITM) and long-term memory (LTM) in Aplysia using an operant learning paradigm. The proteasome inhibitor MG-132 inhibited the induction and molecular consolidation of LTM with no effect on ITM. Remarkably, maintenance of steady-state protein levels through inhibition of protein synthesis using either anisomycin or rapamycin in conjunction with proteasome inhibition permitted the formation of robust 24 h LTM. Our studies suggest a primary role for proteasomal activity in facilitation of gene transcription for LTM and raise the possibility that synaptic mechanisms are sufficient to sustain 24 h memory., (© 2016 Lyons et al.; Published by Cold Spring Harbor Laboratory Press.)
- Published
- 2016
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