1. Antiviral activities of sulfated polysaccharides isolated from Sphaerococcus coronopifolius (Rhodophytha, Gigartinales) and Boergeseniella thuyoides (Rhodophyta, Ceramiales).
- Author
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Bouhlal R, Haslin C, Chermann JC, Colliec-Jouault S, Sinquin C, Simon G, Cerantola S, Riadi H, and Bourgougnon N
- Subjects
- Adsorption drug effects, Animals, Antiviral Agents analysis, Antiviral Agents chemistry, Antiviral Agents isolation & purification, Cell Survival, Chlorocebus aethiops, Galactose analogs & derivatives, Galactose chemistry, Galactose pharmacology, HIV-1 drug effects, Herpesvirus 1, Human drug effects, Humans, Matrix Metalloproteinase 17 drug effects, Molecular Weight, Morocco, Oceans and Seas, Phytotherapy, Plant Preparations pharmacology, Polysaccharides analysis, Polysaccharides chemistry, Polysaccharides isolation & purification, Sulfates chemistry, Sulfates pharmacology, Time Factors, Uronic Acids chemistry, Uronic Acids pharmacology, Vero Cells, Virus Replication drug effects, Antiviral Agents pharmacology, Polysaccharides pharmacology, Rhodophyta chemistry
- Abstract
Water-soluble sulfated polysaccharides isolated from two red algae Sphaerococcus coronopifolius (Gigartinales, Sphaerococcaceae) and Boergeseniella thuyoides (Ceramiales, Rhodomelaceae) collected on the coast of Morocco inhibited in vitro replication of the Human Immunodeficiency Virus (HIV) at 12.5 μg/mL. In addition, polysaccharides were capable of inhibiting the in vitro replication of Herpes simplex virus type 1 (HSV-1) on Vero cells values of EC₅₀ of 4.1 and 17.2 μg/mL, respectively. The adsorption step of HSV-1 to the host cell seems to be the specific target for polysaccharide action. While for HIV-1, these results suggest a direct inhibitory effect on HIV-1 replication by controlling the appearance of the new generations of virus and potential virucidal effect. The polysaccharides from S. coronopifolius (PSC) and B. thuyoides (PBT) were composed of galactose, 3,6-anhydrogalactose, uronics acids, sulfate in ratios of 33.1, 11.0, 7.7 and 24.0% (w/w) and 25.4, 16.0, 3.2, 7.6% (w/w), respectively.
- Published
- 2011
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