1. Whole genome sequencing analysis of multidrug-resistant tuberculosis in Singapore, 2006-2018.
- Author
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Chee CBE, Lim LKY, Ong RTH, Sng LH, Hsu LY, Lee VJM, and Wang YT
- Subjects
- Drug Resistance, Multiple, Bacterial, Genotype, Humans, Molecular Epidemiology, Mycobacterium tuberculosis drug effects, Mycobacterium tuberculosis genetics, Singapore epidemiology, Whole Genome Sequencing, Antitubercular Agents pharmacology, Tuberculosis, Multidrug-Resistant microbiology
- Abstract
There were 290 multidrug-resistant (MDR)-TB cases diagnosed in Singapore from 2006 to 2018. Eighty-one percent were foreign-born. Spoligotyping and MIRU-VNTR methods identified 108 patients in 24 clusters. The Beijing spoligotype accounted for 22 clusters. Whole genome sequencing (WGS) analysis reduced the number of clustered patients and clusters to 43 and nine respectively. One MIRU cluster was redefined into three WGS clusters. All the clusters had foreign-born source cases. Forty percent of local-born, versus 9% of foreign-born, MDR-TB cases belonged to WGS clusters. WGS more accurately elucidated potential MDR-TB transmission which was overestimated by conventional genotyping methods in Singapore.
- Published
- 2021
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