1. Chemical profile and anti-leishmanial activity of three Ecuadorian propolis samples from Quito, Guayaquil and Cotacachi regions.
- Author
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Cuesta-Rubio O, Campo Fernández M, Márquez Hernández I, Jaramillo CGJ, González VH, Montes De Oca Porto R, Marrero Delange D, Monzote Fidalgo L, Piccinelli AL, Campone L, and Rastrelli L
- Subjects
- Animals, Antiprotozoal Agents isolation & purification, Cells, Cultured, Ecuador, Flavonoids isolation & purification, Leishmania drug effects, Macrophages parasitology, Mice, Inbred BALB C, Triterpenes isolation & purification, Antiprotozoal Agents chemistry, Flavonoids chemistry, Propolis chemistry, Triterpenes chemistry
- Abstract
Three propolis samples were collected from different regions of Ecuador (Quito, Guayaquil and Cotacachi) and their methanolic extracts were prepared. Preliminary information supplied by TLC and NMR data, allowed us to define two main types of propolis: Cotacachi propoli sample (CPS), rich in flavonoids and Quito and Guayaquil samples (QPS and GPS) containing triterpenic alcohols and acetyl triterpenes as the main constituents. Two different approaches based on RP-HPLC preparative procedure and NMR structural determination (CPS) and GC-MS analysis (QPS and GPS) were successfully used for the chemical characterization of their major compounds. All three propolis extracts were able to inhibit Leishmania amazonensis growth but propolis sample rich in flavonoids was the most active (IC
50 =17.1±1.7μg/mL). In the literature this is the first study on propolis from Ecuador., (Copyright © 2017. Published by Elsevier B.V.)- Published
- 2017
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