67 results on '"Salmen, A."'
Search Results
2. Differential responses of chili varieties grown under cadmium stress
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Sana, Sundas, Ramzan, Musarrat, Ejaz, Samina, Danish, Subhan, Salmen, Saleh H., and Ansari, Mohammad Javed
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- 2024
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3. Effect of silicon nanoparticle-based biochar on wheat growth, antioxidants and nutrients concentration under salinity stress
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Sidra Gill, Musarrat Ramzan, Gul Naz, Liaqat Ali, Subhan Danish, Mohammad Javed Ansari, and Saleh H. Salmen
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Antioxidants ,Biochar ,Macronutrients ,Micronutrients ,Salinity stress ,Silicon nanoparticles ,Medicine ,Science - Abstract
Abstract Globally, salinity is an important abiotic stress in agriculture. It induced oxidative stress and nutritional imbalance in plants, resulting in poor crop productivity. Applying silicon (Si) can improve the uptake of macronutrients. On the other hand, using biochar as a soil amendment can also decrease salinity stress due to its high porosity, cation exchange capacity, and water-holding capacity. That’s why the current experiment was conducted with novelty to explore the impact of silicon nanoparticle-based biochar (Si-BC) on wheat cultivated on salt-affected soil. There were 3 levels of Si-BC, i.e., control (0), 1% Si-BC1, and 2.5% Si-BC2 applied in 3 replicates under 0 and 200 mM NaCl following a completely randomized design. Results showed that treatment 2.5% Si-BC2 performed significantly better for the enhancement in shoot and root length, shoot and root fresh weight, shoot and root dry weight, number of leaves, number of tillers, number of spikelets, spike length, spike fresh and dry weight compared to control under no stress and salinity stress (200 mM NaCl). A significant enhancement in chlorophyll a (~ 18%), chlorophyll b (~ 22%), total chlorophyll (~ 20%), carotenoid (~ 60%), relative water contents (~ 58%) also signified the effectiveness of treatment 2.5% Si-BC2 than control under 200 mM NaCl. In conclusion, treatment 2.5% Si-BC2 can potentially mitigate the salinity stress in wheat by regulating antioxidants and improving N, K concentration, and gas exchange attributes while decreasing Na and Cl concentration and electrolyte leakage. More investigations at the field level are recommended for the declaration of treatment 2.5% Si-BC2 as the best amendment for alleviating salinity stress in different crops under variable climatic conditions.
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- 2024
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4. Differential responses of chili varieties grown under cadmium stress
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Sundas Sana, Musarrat Ramzan, Samina Ejaz, Subhan Danish, Saleh H. Salmen, and Mohammad Javed Ansari
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Cadmium ,Capsicum annuum L ,Flavonoids ,Antioxidants ,Total soluble sugars ,Total proteins ,Botany ,QK1-989 - Abstract
Abstract Heavy metal cadmium (Cd) naturally occurs in soil and is a hazardous trace contaminant for humans, animals, and plants. The main sources of Cd pollution in soil include overuse of phosphatic fertilizers, manure, sewage sludge, and aerial deposition. That’s why an experiment was conducted to analyze the effect of Cd toxicity in Capsicum annuum L. by selecting its seven varieties: Hybrid, Desi, Sathra, G-916, BR-763, BG-912, and F1-9226. Cadmium was spiked in soil with four levels, i.e., (0, 3, 4, and 5 mg Cd kg− 1 of soil) for a week for homogeneous dispersion of heavy metal. Chili seeds were sown in compost-filled loamy soil, and 25-day-old seedlings were transplanted into Cd-spiked soil. Cadmium increasing concentration in soil decreased chili growth characteristics, total soluble sugars, total proteins, and amino acids. On the other hand, the activities of antioxidant enzymes were increased with the increasing concentration of Cd in almost all the varieties. Treatment 5 mg Cd/kg application caused − 197.39%, -138.78%, -60.77%, -17.84%, -16.34%, -11.82% and − 10.37% decrease of carotenoids level in chili V2 (Desi) followed by V4 (G-916), V1 (Hy7brid), V7 (F1-9226), V6 (BG-912), V5 (BR-763) and V3 (Sathra) as compared to their controls. The maximum flavonoids among varieties were in V5 (BR-763), followed by V6 (BG-912), V7 (F1-9226), V3 (Sathra) and V1 (Hybrid). Flavonoids content was decreased with − 37.63% (Sathra), -34.78% (Hybrid), -33.85% (G-916), -31.96% (F1-9226), -31.44% (Desi), -30.58% (BR-763), -22.88% (BG-912) as compared to their control at 5 mg Cd/kg soil stress. The maximum decrease in POD, SOD, and CAT was − 31.81%, -25.98%, -16.39% in chili variety V7 (F1-9226) at 5 mg Cd/kg stress compared to its control. At the same time, maximum APX content decrease was − 82.91%, followed by -80.16%, -65.19%, -40.31%, -30.14%, -10.34% and − 6.45% in V4 (G-916), V2 (Desi), V3 (Sathra), V6 (BG-912), V1 (Hybrid), V7 (F1-9226) and V5 (BR-763) at 5 mg Cd/kg treatment as compared to control chili plants. The highest CAT was found in 5 chili varieties except Desi and G-916. Desi and G-916 varieties. V5 (BR-763) and V6 (BG-912) were susceptible, while V1 (Hybrid), V3 (Sathra), and V7 (F1-9226) were with intermediate growth attributes against Cd stress. Our results suggest that Desi and G-916 chili varieties are Cd tolerant and can be grown on a large scale to mitigate Cd stress naturally.
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- 2024
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5. Modulation of sunflower growth via regulation of antioxidants, oil content and gas exchange by arbuscular mycorrhizal fungi and quantum dot biochar under chromium stress
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Musarrat Ramzan, Talha Jamshaid, Liaqat Ali, Khadim Dawar, Rabia Saba, Usama Jamshaid, Shah Fahad, Saleh H. Salmen, Mohammad Javed Ansari, Subhan Danish, Misbah Hareem, Hina Saif, and Khurrum Shahzad
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Antioxidants ,Chromium ,Chlorophyll content ,Gas exchange ,Oil content ,Sunflower ,Botany ,QK1-989 - Abstract
Abstract Chromium (Cr) toxicity significantly threatens sunflower growth and productivity by interfering with enzymatic activity and generating reactive oxygen species (ROS). Zinc quantum dot biochar (ZQDB) and arbuscular mycorrhizal fungi (AMF) have become popular to resolve this issue. AMF can facilitate root growth, while biochar tends to minimize Cr mobility in soil. The current study aimed to explore AMF and ZQDB combined effects on sunflower plants in response to Cr toxicity. Four treatments were applied, i.e. NoAMF + NoZQDB, AMF + 0.40%ZQDB, AMF + 0.80%ZQDB, and AMF + 1.20%ZQDB, under different stress levels of Cr, i.e. no Cr (control), 150 and 200 mg Cr/kg soil. Results showed that AMF + 1.20%ZQDB was the treatment that caused the greatest improvement in plant height, stem diameter, head diameter, number of leaves per plant, achenes per head, 1000 achenes weight, achene yield, biological yield, transpiration rate, stomatal conductance, chlorophyll content and oleic acid, relative to the condition NoAMF + No ZQDB at 200 mg Cr/kg soil. A significant decline in peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT) while improvement in ascorbate peroxidase (APx), oil content, and protein content further supported the effectiveness of AMF + 1.20%ZQDB against Cr toxicity. Our results suggest that the treatment AMF + 1.20%ZQDB can efficiently alleviate Cr stress in sunflowers.
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- 2023
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6. Modulation of sunflower growth via regulation of antioxidants, oil content and gas exchange by arbuscular mycorrhizal fungi and quantum dot biochar under chromium stress
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Ramzan, Musarrat, Jamshaid, Talha, Ali, Liaqat, Dawar, Khadim, Saba, Rabia, Jamshaid, Usama, Fahad, Shah, Salmen, Saleh H., Ansari, Mohammad Javed, Danish, Subhan, Hareem, Misbah, Saif, Hina, and Shahzad, Khurrum
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- 2023
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7. Antioxidants in Hops: Bioavailability, Health Effects and Perspectives for New Products
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Corina-Aurelia Zugravu, Roxana-Elena Bohiltea, Teodor Salmen, Elena Pogurschi, and Marina Ruxandra Otelea
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hops ,antioxidants ,metabolic syndrome ,bioavailability ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Hop plant (Humulus lupulus L.) has been used by humans for ages, presumably first as a herbal remedy, then in the manufacturing of different products, from which beer is the most largely consumed. Female hops cones have different useful chemical compounds, an important class being antioxidants, mainly polyphenols. This narrative review describes the main antioxidants in hops, their bioavailability and biological effects, and the results obtained by now in the primary and secondary prevention of several non-communicable diseases, such as the metabolic syndrome related diseases and oncology. This article presents in vitro and in vivo data in order to better understand what was accomplished in terms of knowledge and practice, and what needs to be clarified by additional studies, mainly regarding xantohumol and its derivates, as well as regarding the bitter acids of hops. The multiple protective effects found by different studies are hindered up to now by the low bioavailability of some of the main antioxidants in hops. However, there are new promising products with important health effects and perspectives of use as food supplements, in a market where consumers increasingly search for products originating directly from plants.
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- 2022
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8. Antibacterial, antifungal, antidiabetic, and antioxidant activities potential of Coleus aromaticus synthesized titanium dioxide nanoparticles
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Wongchai, Anupong, Ruangwong, On-Uma, Kumchai, Jutamas, Saleh H, Salmen, Sulaiman Ali, Alharbi, Deepika, Joshi, and G K, Jhanani
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Titanium ,Staphylococcus aureus ,Antifungal Agents ,Metal Nanoparticles ,alpha-Glucosidases ,Biochemistry ,Antioxidants ,Anti-Bacterial Agents ,Coleus ,Pseudomonas aeruginosa ,Escherichia coli ,Hypoglycemic Agents ,Nanoparticles ,General Environmental Science - Abstract
The nanoparticles based drug delivery and treatment related research has been increased significantly in the recent years. Hence, the antibacterial, antifungal, and antioxidant activity potential of pre synthesized and characterized Titanium dioxide nanoparticles (TiO
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- 2023
9. Cobalt nanoparticles synthesizing potential of orange peel aqueous extract and their antimicrobial and antioxidant activity
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Wongchai, Anupong, Ruangwong, On-Uma, Kumchai, Jutamas, Deepika, Joshi, Saleh H, Salmen, Tahani Awad, Alahmadi, and G K, Jhanani
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Silver ,Anti-Infective Agents ,Plant Extracts ,Escherichia coli ,Metal Nanoparticles ,Microbial Sensitivity Tests ,Cobalt ,Hydrogen Peroxide ,Biochemistry ,Antioxidants ,Citrus sinensis ,Anti-Bacterial Agents ,General Environmental Science - Abstract
The ability of cobalt nanoparticles (CoNPs) to absorb electromagnetic waves led to their use as potential biomedical agents in recent years. The properties of magnetic fluid containing cobalt nanoparticles are extraordinary. Hence, this research was designed to evaluate the Co(NO
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- 2023
10. Characterization of NiONPs synthesized by aqueous extract of orange fruit waste and assessed their antimicrobial and antioxidant potential
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Mathiyazhagan, Narayanan, Natarajan, Devarajan, Saleh H, Salmen, Sulaiman Ali, Alharbi, Rupa, Lavarti, Nguyen Thuy, Lan Chi, and Kathirvel, Brindhadevi
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Staphylococcus aureus ,Anti-Infective Agents ,Fruit ,Escherichia coli ,Metal Nanoparticles ,Microbial Sensitivity Tests ,Hydrogen Peroxide ,Biochemistry ,Antioxidants ,Anti-Bacterial Agents ,General Environmental Science - Abstract
This research was performed to evaluate the nickel oxide nanoparticles (NiONPs) fabricating potential of orange fruit waste (OFW) aqueous extract. Moreover characterize the synthesized OFW-NiONPs through standard techniques such as UV-vis. spectrophotometer, Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS) and Scanning Electron Microscope (SEM) analyses. Furthermore, the antimicrobial and antioxidant potential of OFW-NiONPs were studied against most common microbial pathogens (Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, and Aspergillus niger) and free radicals (2,2-diphenyl-1-picrylhydrazyl (DPPH), H
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- 2023
11. Identification of bioactive peptides from a Brazilian kefir sample, and their anti-Alzheimer potential in Drosophila melanogaster
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Serena Mares Malta, Letícia Leandro Batista, Heitor Cappato Guerra Silva, Rodrigo Rodrigues Franco, Matheus Henrique Silva, Tamiris Sabrina Rodrigues, Lucas Ian Veloso Correia, Mário Machado Martins, Gabriela Venturini, Foued Salmen Espindola, Murilo Vieira da Silva, and Carlos Ueira-Vieira
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Molecular Docking Simulation ,Proteomics ,Multidisciplinary ,Amyloid beta-Peptides ,Drosophila melanogaster ,Kefir ,Alzheimer Disease ,Acetylcholinesterase ,Animals ,Dysbiosis ,Peptides ,Antioxidants ,Brazil - Abstract
Alzheimer’s disease (AD) is the most common form of dementia in the elderly, affecting cognitive, intellectual, and motor functions. Different hypotheses explain AD’s mechanism, such as the amyloidogenic hypothesis. Moreover, this disease is multifactorial, and several studies have shown that gut dysbiosis and oxidative stress influence its pathogenesis. Knowing that kefir is a probiotic used in therapies to restore dysbiosis and that the bioactive peptides present in it have antioxidant properties, we explored its biotechnological potential as a source of molecules capable of modulating the amyloidogenic pathway and reducing oxidative stress, contributing to the treatment of AD. For that, we used Drosophila melanogaster model for AD (AD-like flies). Identification of bioactive peptides in the kefir sample was made by proteomic and peptidomic analyses, followed by in vitro evaluation of antioxidant and acetylcholinesterase inhibition potential. Flies were treated and their motor performance, brain morphology, and oxidative stress evaluated. Finally, we performed molecular docking between the peptides found and the main pathology-related proteins in the flies. The results showed that the fraction with the higher peptide concentration was positive for the parameters evaluated. In conclusion, these results revealed these kefir peptide-rich fractions have therapeutic potential for AD.
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- 2022
12. B procyanidins of Annona crassiflora fruit peel inhibited glycation, lipid peroxidation and protein-bound carbonyls, with protective effects on glycated catalase
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André Lopes Saraiva, Raquel M. F. Sousa, Heitor Cappato Guerra Silva, Foued Salmen Espindola, Rodrigo Rodrigues Franco, and Allisson Benatti Justino
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Glycation End Products, Advanced ,0301 basic medicine ,Glycosylation ,Antioxidant ,medicine.medical_treatment ,Phytochemicals ,lcsh:Medicine ,medicine.disease_cause ,Biochemistry ,Annona ,Antioxidants ,Catechin ,Protein Carbonylation ,Lipid peroxidation ,chemistry.chemical_compound ,0302 clinical medicine ,Glycation ,lcsh:Science ,chemistry.chemical_classification ,Multidisciplinary ,biology ,Biological techniques ,Serum Albumin, Bovine ,Catalase ,Cross-Linking Reagents ,030220 oncology & carcinogenesis ,Thiol ,Cell Survival ,Protective Agents ,Fluorescence ,Article ,Cell Line ,03 medical and health sciences ,Phenols ,medicine ,Animals ,Biflavonoids ,Proanthocyanidins ,Sulfhydryl Compounds ,Reactive oxygen species ,lcsh:R ,Annona crassiflora ,biology.organism_classification ,030104 developmental biology ,chemistry ,Fruit ,biology.protein ,Cattle ,lcsh:Q ,Lipid Peroxidation ,Reactive Oxygen Species ,Plant sciences ,Oxidative stress - Abstract
Advanced glycation end-products (AGEs) have been reported as results of increased oxidative stress. Consequently, the search for new antioxidant and anti-glycating agents is under intense investigation. Plant-derived procyanidins have previously demonstrated anti-glycation properties. Thus, this study aimed to isolate procyanidins from Annona crassiflora fruit peel, a species from the Brazilian Savanna, and investigate their antioxidant and anti-glycation effects. Free radical scavenging and quenching properties, formation of reactive oxygen species (ROS), AGEs, protein carbonyl and thiol groups, lipid peroxidation, crosslinked AGEs, as well as glycated catalase activity, were analyzed. In addition, in silico assessment of absorption, distribution, metabolism, excretion and toxicity was carried out. The procyanidins-enriched fraction, named here as F7, showed high antioxidant and anti-glycation capacities, with inhibitory activities against lipid peroxidation, and AGEs and ROS formation. In addition, there were reductions in AGEs-induced crosslinks and protein carbonyls and protective effects against oxidation of thiol groups and glycated-catalase. ADMET predictions of F7 showed favorable absorption and distribution, with no hepatotoxicity or mutagenicity. Together, our results support the anti-glycation activities of the procyanidins-enriched fraction from A. crassiflora, and suggest that these effects are triggered, at least in part, by scavenging free radical and dicarbonyls intermediates.
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- 2019
13. Increased selenium concentration in the synthesis of CdSe magic-sized quantum dots affects how the brain responds to oxidative stress
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Danielle Diniz Vilela, Allisson Benatti Justino, Douglas Carvalho Caixeta, Adriele Vieira Souza, Renata Roland Teixeira, Rodrigo Rodrigues Franco, André Lopes Saraiva, Belchiolina Beatriz Fonseca, Noelio Oliveira Dantas, Anielle Christine Almeida Silva, and Foued Salmen Espindola
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Superoxide Dismutase ,Biomedical Engineering ,Brain ,Chick Embryo ,Glutathione ,Antioxidants ,Biomaterials ,Oxidative Stress ,Selenium ,Quantum Dots ,Cadmium Compounds ,Animals ,Selenium Compounds ,Cadmium - Abstract
CdSe magic-sized quantum dots (MSQDs) have been widely used as fluorescent probes in biological systems due to their excellent optical properties with a broader fluorescence spectrum and stable luminescence in biological media. However, they can be cytotoxic and alter the redox balance depending on the amounts of Cd
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- 2021
14. Topic use of Annona crassiflora Mart. contributes to wound healing due to the antioxidant and proliferative effects of fibroblasts
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Francyelle Borges Rosa de Moura, Bruno Antonio Ferreira, Elusca Helena Muniz, Allisson Benatti Justino, Ana Gabriela Silva, Rinara de Almeida Santos, José Augusto Leoncio Gomide, Rosy Iara Maciel de Azambuja Ribeiro, Daniele Lisboa Ribeiro, Fernanda de Assis Araújo, Foued Salmen Espindola, and Tatiana Carla Tomiosso
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Mice ,Wound Healing ,Plant Extracts ,General Earth and Planetary Sciences ,Animals ,Fibroblasts ,Annona ,Antioxidants ,General Environmental Science - Abstract
Annona crassiflora Mart. is a species native to the Cerrado biome, whose fruit is known as araticum or marolo. Plant parts are widely used in folk medicine to treat inflammation and pain associated with rheumatism, wounds, venereal diseases, snakebites, and microbial infections. Thus, we investigated a fraction rich in phenolic compounds (PCAc) obtained from the crude extract of the peel of these fruits on non-cytotoxic, anti-inflammatory, antioxidant, and collagen biosynthesis properties in the healing of wounds induced on the back of BALB/c mice. For the control group, the induced wounds were not treated and for the others, wounds were treated topically with vehicle or vehicle plus PCAc. Both fractions contained in PCAc demonstrated effective protection on fibroblasts. We highlight the effect of the ethyl acetate fraction which, in addition to the protective effect, has a proliferative activity on these cells. In addition, PCAc caused improvement in healing after 7 days of treatment and in the longest period of treatment with PCAc (7, 14, and 21 days) there was a greater contraction of the wound, accompanied by resolution of the inflammatory process, antioxidant defense, increasing collagen synthesis, and modulation of metalloproteinases. PCAc demonstrated better re-epithelialization and organization of the dermis at the end of treatment. The changes promoted by the phenolic compounds of A. crassiflora were important in the healing process, especially in activities related to inflammation, oxidative stress, and fibrogenesis.
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- 2021
15. Flavonoids and proanthocyanidins-rich fractions from Eugenia dysenterica fruits and leaves inhibit the formation of advanced glycation end-products and the activities of α-amylase and α-glucosidase
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Allisson Benatti Justino, Heitor Cappato Guerra Silva, Rodrigo Rodrigues Franco, Isabela de Oliveira Cavalcante Pimentel, Nicole Fernandes Silva, André Lopes Saraiva, and Foued Salmen Espindola
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Pharmacology ,Flavonoids ,Glycation End Products, Advanced ,Cell Survival ,Macrophages ,Polyphenols ,alpha-Glucosidases ,Eugenia ,Antioxidants ,Gene Expression Regulation, Enzymologic ,Mice, Inbred C57BL ,Plant Leaves ,Mice ,Fruit ,Drug Discovery ,Animals ,Proanthocyanidins ,Enzyme Inhibitors ,alpha-Amylases - Abstract
Different parts of Eugenia dysenterica have been popularly used in Brazil for treating diabetes mellitus and its complications. The present study aimed to screen extracts from E. dysenterica fruit pulp, peel, seed and leaf for carbohydrate digestive enzymes inhibitors with antioxidant and anti-glycation capacities.Ethanol extracts of E. dysenterica were subjected to a liquid-liquid fractionation and the fractions were used to evaluate their antioxidant properties and inhibitory potential against the formation of advanced glycation end-products (AGEs) and α-amylase and α-glucosidase.The ethyl acetate fraction (EtOAcF) from seed and the dichloromethane fraction (CHThe results strongly contribute to the understanding of the antidiabetic potential of seeds and leaves from E. dysenterica, a species from a global biodiversity hotspot, which appears to be linked to the prevention of oxidative stress, AGEs production and postprandial hyperglycemia.
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- 2021
16. CdSe magic-sized quantum dots attenuate reactive oxygen species generated by neutrophils and macrophages with implications in experimental arthritis
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André Lopes Saraiva, Thiago Neves Vieira, Ana Flávia Oliveira Notário, João Paulo Mesquita Luiz, Cássia Regina Silva, Luiz Ricardo Goulart, Noelio Oliveira Dantas, Anielle Christine Almeida Silva, and Foued Salmen Espindola
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Neutrophils ,Macrophages ,Biomedical Engineering ,Pharmaceutical Science ,Medicine (miscellaneous) ,Bioengineering ,Antioxidants ,Arthritis, Rheumatoid ,Quantum Dots ,Cadmium Compounds ,Molecular Medicine ,Humans ,General Materials Science ,Reactive Oxygen Species ,Selenium Compounds - Abstract
The biological applicability of nanomaterials has been limited due to cytotoxicity. Studies have described the effects of nanomaterials on different tissues and cell types, but their actions on immune cells are less elucidated. This study describes unprecedented in vitro and in vivo antioxidant activities of cadmium selenide magic-sized quantum dots (CdSe MSQDs) with implications on rheumatoid arthritis. While the generation of ROS induced by nanomaterials is linked to cytotoxicity, we found that CdSe MSQDs reduced ROS production by neutrophils and macrophages following opsonized-zymosan stimuli, and we did not find cytotoxic effects. Interestingly, inherent antioxidant properties of CdSe MSQDs were confirmed through DPPH, FRAP, and ORAC assays. Furthermore, CdSe MSQDs reduced ROS levels generated by infiltrating leukocytes into joints in experimental model of rheumatoid arthritis. Briefly, we describe a novel application of CdSe MSQDs in modulating the inflammatory response in experimental rheumatoid arthritis through an unexpected antioxidant activity.
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- 2021
17. Antioxidant compounds from Annona crassiflora fruit peel reduce lipid levels and oxidative damage and maintain the glutathione defense in hepatic tissue of Triton WR-1339-induced hyperlipidemic mice
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Francyelle Borges Rosa de Moura, Natália Tavernelli, Rodrigo Rodrigues Franco, André Lopes Saraiva, Heitor Cappato Guerra Silva, Foued Salmen Espindola, Adriele Vieira de Souza, Françoise Vasconcelos Botelho, Allisson Benatti Justino, and Letícia Pereira Afonso Ramos
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Male ,Glutathione system ,Antioxidant ,medicine.medical_treatment ,Hyperlipidemias ,RM1-950 ,Pharmacology ,medicine.disease_cause ,Annona ,Antioxidants ,Polyethylene Glycols ,Lipid peroxidation ,Protein Carbonylation ,chemistry.chemical_compound ,Antioxidant activity ,Hyperlipidemia ,medicine ,Animals ,Triglycerides ,Hypolipidemic Agents ,Chemistry ,Cholesterol ,Plant Extracts ,Hypertriglyceridemia ,Polyphenols ,General Medicine ,Glutathione ,medicine.disease ,Lipid Metabolism ,Phenolic compounds ,Mice, Inbred C57BL ,Oxidative Stress ,Dyslipidemia ,Liver ,Fruit ,Therapeutics. Pharmacology ,Lipid Peroxidation ,Oxidoreductases ,Oxidative stress ,Lipoprotein - Abstract
Dyslipidemia is a risk factor for the pathogenesis of several diseases, such as obesity, hypertension, atherosclerosis and cardiovascular diseases. In addition to interfering with serum concentrations of cholesterol and triglycerides, hyperlipidemia is involved in oxidative stress increase and reduction of the endogenous antioxidant defenses. The fruit peel of Annona crassiflora crude extract (CEAc) and its polyphenols-rich fraction (PFAc) were investigated against hypertriglyceridemia, hypercholesterolemia and hepatic oxidative stress in Triton WR-1339-induced hyperlipidemic mice. Lipid parameters in serum, feces and liver, as well as hepatic oxidative status, and enzymatic and non-enzymatic antioxidant defense systems were analyzed. Pre-treatment with CEAc for 12 days decreased hepatic triglycerides and total cholesterol, and similar to PFAc, increased the high-density lipoprotein level. There were reductions in lipid peroxidation and protein carbonylation, as well as restoration of the glutathione defense system and total thiol content in the liver of the hyperlipidemic mice treated with PFAc. The fruit peel of A. crassiflora, a promising natural source of bioactive molecules, showed a potential lipid-lowering action and hepatoprotective activities triggered by reduction of oxidative damage and maintenance of the enzymatic and non-enzymatic antioxidant systems impaired by the hyperlipidemic state.
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- 2021
18. Stephalagine, an Aporphinic Alkaloid with Therapeutic Effects in Acute Gout Arthritis in Mice
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Priscilla Dias Santos, Thiago Neves Vieira, Ana Claudia Gontijo Couto, João Paulo Mesquita Luiz, André Luis Lopes Saraiva, Camila Rodrigues Borges Linhares, Marília Fontes Barbosa, Allisson Benatti Justino, Rodrigo Rodrigues Franco, Evelyne da Silva Brum, Sara Marchesan Oliveira, Paula Dechichi, Marcos Pivatto, Veridiana de Melo Rodrigues Ávila, Foued Salmen Espíndola, and Cássia Regina Silva
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Male ,Pharmacology ,Analgesics ,Aporphines ,Gout ,Arthritis, Gouty ,Anti-Inflammatory Agents ,Pain ,Antioxidants ,Mice, Inbred C57BL ,Mice ,Alkaloids ,Hyperalgesia ,Drug Discovery ,Animals ,Edema ,Peroxidase - Abstract
Gout is an inflammatory disease characterized by the accumulation of monosodium urate crystals (MSU) in the joints, leading to severe pain and inflammation. Stephalagine is a Brazilian Savanna aporphine alkaloid isolated from Annona crassiflora Mart. Fruit peel, that has been popularly used to treat rheumatism and have been described with antinociceptive properties. However, no studies evaluated the possible therapeutic properties of stephalagine in arthritic pain.To evaluate the possible antinociceptive and anti-inflammatory effects of stephalagine in an acute gout attack in mice.Adult male wild type C57BL/6/J/UFU mice (20-25 g) were used (process number 018/17). The treated group received stephalagine (1 mg/kg, by gavage) and the vehicle group received saline (10 mL/kg, by gavage), both 1 h before the MSU crystals (100 μg/ankle joint) administration. All groups were analyzed for mechanical allodynia, thermal hyperalgesia, overt pain-like behaviors, and edema development at 2, 4, 6 and 24 h after injections. Synovial fluid and the ankle articulation from the injected joint were collected 4 h after administrations for myeloperoxidase enzyme activity, IL-1β measurement, and histological analysis.Stephalagine had a significant antinociceptive effect on mechanical allodynia, when compared to vehicle group at 2-24 h after intra-articular injection of MSU and 2 h for spontaneous and cold thermal sensitivity. Stephalagine was also able to significantly reduce the articular edema (45 ± 1%), the activity of the myeloperoxidase enzyme (37 ± 6%), and IL-1β levels (43 ± 3%). The histological analysis confirms that stephalagine dramatically reduced the number of infiltrating inflammatory cells (75 ± 6%) in MSU injected animals. Also, stephalagine treatment did not alter the uric acid levels, xanthine oxidase activity, AST and ALT activities, urea and creatinine levels, neither cause any macroscopic changes in the mice's weight, deformations, changes in the coat, or feces.Stephalagine may be an alternative for the management of gout, once it was able to induce antinociceptive and anti-inflammatory effects without causing adverse effects on the evaluated parameters.
- Published
- 2021
19. Antioxidant compounds of Kielmeyera coriacea Mart. with α-amylase, lipase and advanced glycation end-product inhibitory activities
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Sérgio Antônio Lemos de Morais, André Lopes Saraiva, Alberto de Oliveira, Francisco José Tôrres de Aquino, Joed Pires de Lima Júnior, Julia Silveira Queiroz, Foued Salmen Espindola, Heitor Cappato Guerra Silva, Rodrigo Rodrigues Franco, Mário Machado Martins, Luiz Ricardo Goulart Filho, Eder C. Santana, and Allisson Benatti Justino
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Glycation End Products, Advanced ,Antioxidant ,DPPH ,medicine.medical_treatment ,Clinical Biochemistry ,Pharmaceutical Science ,Antioxidants ,Analytical Chemistry ,chemistry.chemical_compound ,Glycation ,Drug Discovery ,medicine ,Hypoglycemic Agents ,Amylase ,Food science ,Spectroscopy ,biology ,Plant Extracts ,Kielmeyera coriacea ,Lipase ,biology.organism_classification ,chemistry ,Polyphenol ,biology.protein ,Advanced glycation end-product ,Trolox ,alpha-Amylases - Abstract
Chronic hyperglycemia and hyperlipidemia are associated with excessive formation of reactive oxygen species and advanced glycation end-products. The present study aimed to evaluate the potential in vitro antidiabetic properties of Kielmeyera coriacea inner bark. The main phytochemical compounds were identified by UHPLC-ESI/MSn and the ethanol extract and its fractions were used to evaluate their antioxidant and anti-glycation capacities, as well as their inhibitory potential against glycoside and lipid hydrolases activities. The polar fractions, especially the n-butanol fraction, had free radical scavenging and quenching properties (ORAC and FRAP values>1800 and 1000 µmol trolox eq/g, respectively, and DPPH IC50
- Published
- 2020
20. Overweight Women with Breast Cancer on Chemotherapy Have More Unfavorable Inflammatory and Oxidative Stress Profiles
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Foued Salmen Espindola, Carlos Eduardo Paiva, James R. Hébert, Izabella C C Ferreira, Douglas Carvalho Caixeta, Eduarda da Costa Marinho, Yara Cristina de Paiva Maia, Alinne T F Silva, Letícia L D Santos, Adriele Vieira de Souza, Nitin Shivappa, Luiz Ricardo Goulart, Renata Roland Teixeira, Isis Danyelle Dias Custódio, and Thaise Gonçalves Araújo
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0301 basic medicine ,chronic inflammation ,Antioxidant ,medicine.medical_treatment ,Glutathione reductase ,Physiology ,Overweight ,medicine.disease_cause ,Antioxidants ,chemistry.chemical_compound ,0302 clinical medicine ,Prospective Studies ,Prospective cohort study ,Nutrition and Dietetics ,Middle Aged ,030220 oncology & carcinogenesis ,dietary inflammatory index ,Female ,medicine.symptom ,Diet, Healthy ,Inflammation Mediators ,lcsh:Nutrition. Foods and food supply ,Oxidation-Reduction ,Adult ,dietary total antioxidant capacity ,Nutritional Status ,lcsh:TX341-641 ,Antineoplastic Agents ,Breast Neoplasms ,Diet Surveys ,Article ,03 medical and health sciences ,Breast cancer ,breast cancer ,medicine ,Humans ,Aged ,Inflammation ,Chemotherapy ,business.industry ,Glutathione ,medicine.disease ,Oxidative Stress ,030104 developmental biology ,chemistry ,plasma biomarkers ,business ,Oxidative stress ,Biomarkers ,Food Science - Abstract
Chronic inflammation and redox imbalance are strongly influenced by diet and nutritional status, and both are risk factors for tumor development. This prospective study aimed to explore the associations between inflammatory and antioxidant markers and nutritional status in women with breast cancer undergoing chemotherapy. The women were evaluated at three times: T0, after the infusion of the first cycle, T1, after infusion of the intermediate cycle, and T2, after the infusion of the last chemotherapy cycle. The consumption of antioxidant nutrients and the Total Dietary Antioxidant Capacity reduced between T0 and T2 and the Dietary Inflammatory Index scores increased throughout the chemotherapy. Blood samples taken at the end of the chemotherapy showed lower levels of glutathione reductase and reduced glutathione, with greater quantification of the transcripts for Interleukin-6 and Tumor Necrosis Factor &alpha, It should be emphasized that the Total Dietary Antioxidant Capacity is lower and the Dietary Inflammatory Index is higher in the group of overweight patients at the end of the follow-up, besides showing lower levels of the redox status, especially the plasma levels of glutathione reductase (p = 0.039). In addition, trends towards higher transcriptional levels of cytokines in peripheral blood were observed more often in overweight women than in non-overweight women. In this study of 55 women with breast cancer, nine (16%) with metastases, diet became more pro-inflammatory with fewer antioxidants during the chemotherapy. Briefly, we have shown that chemotherapy is critical for high-risk overweight women due to their reduced intake of antioxidant nutrients, generating greater inflammatory and oxidative stress profiles, suggesting the adoption of healthier dietary practices by women with breast cancer throughout their chemotherapy.
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- 2020
21. Anacardium humile St. Hil as a novel source of antioxidant, antiglycation and α-amylase inhibitors molecules with potential for management of oxidative stress and diabetes
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Foued Salmen Espindola, Joed Pires de Lima Júnior, Izabela Barbosa Moraes, André Luis Lopes Saraiva, and Rodrigo Rodrigues Franco
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Antioxidant ,DPPH ,medicine.medical_treatment ,Antioxidants ,Anacardium humile ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Drug Discovery ,medicine ,Diabetes Mellitus ,Animals ,Hypoglycemic Agents ,Anacardium ,Glycoside Hydrolase Inhibitors ,Gallic acid ,030304 developmental biology ,Pharmacology ,0303 health sciences ,biology ,Traditional medicine ,Dose-Response Relationship, Drug ,Chemistry ,Plant Extracts ,Catechin ,biology.organism_classification ,Mice, Inbred C57BL ,Oxidative Stress ,Polyphenol ,030220 oncology & carcinogenesis ,Trolox ,alpha-Amylases ,Quercetin - Abstract
Ethnopharmacological relevance The substantial increase in diabetes cases worldwide has been a major public health problem, and the use of medicinal plants can be considered an interesting alternative to control the disease and its complications. Anacardium humile St. Hill. (Anacardiaceae) is a typical plant from the Brazilian savanna, popularly known for its antidiarrheal, expectorant, antidiabetic and anti-inflammatory properties, however, few studies have fully described its biological properties. This study aimed to investigate in vitro and ex vivo the antioxidant and antiglycation potential of A. humile ethanolic extract, its organic fractions and three isolated molecules (quercetin, catechin and gallic acid), their capacity to inhibit the glycolytic enzyme α-amylase, as well as their cytotoxic effects against RAW264.7 macrophages. Material and methods The ethanolic extract of A. humile, its organic fractions and three isolated molecules (catechin, quercetin and gallic acid) were tested for their antioxidant (ORAC, FRAP and DPPH) and antiglycation (BSA/Fructose, BSA/Methylglyoxal, Arginine/Methylglyoxal and Lysine/Methylglyoxal) capacities, and also for its potential to inhibit the enzyme α-amylase. Additionally, bioactive compounds present in the A. humile leaves fractions were elucidated by an HPLC-ESIMS/MS analysis. Results The analysis showed relevant antioxidant activity of DCM (1264.85 ± 76.90 μM Trolox eq/g ORAC; 216.71 ± 1.04 μM Trolox eq/g FRAP and 3.03 ± 0.08 IC50 μg/mL IC50 DPPH) and EtOAc (1300.11 ± 33.04 ORAC, 236.21 ± 23.86 FRAP and 3.03 ± 0.14 μg/mL IC50 DPPH) fractions and also of the isolated molecules, mainly gallic acid (1291.19 ± 8.41 μM Trolox eq/g ORAC, 1103.52 ± 31.48 μM Trolox eq/g FRAP and 0.78 ± 0.11 μg/mL IC50 DPPH). Concerning the antiglycation activity, all samples inhibited over 88% in the BSA-FRU method. In the BSA-MGO and ARG-MGO methods, the Hex, DCM, EtOAc fractions and the isolated molecule catechin stood out. However, in the LYS-MGO model, only the isolated molecules showed significant results. In α-amylase assay, all fractions, for exception Hex, presented notable inhibition capacity with low IC50 values, especially DCM, EtOAc, ButOH and H2O (IC50 0.56 ± 0.10, 0.84 ± 0.01, 0.74 ± 0.03 and 0.79 ± 0.06 μg/mL, respectively). Tests using hepatic tissue showed a notorious capacity of the DCM, AcOEt and ButOH fractions, as well as of the isolated molecules to inhibit lipid peroxidation and ROS production, and also to preserve thiol groups. Molecules of great antioxidant potential were found in our samples, such as kaempferol, quercetin, catechin, gallic acid and luteolin. Conclusion A. humile extract and its organic fractions showed promising antioxidant and antiglycation potential and a prominent capacity to inhibit the α-amylase enzyme. Hence, this study presents new results and stimulates further research to elucidate the biological properties of A. humile and its capacity to manage DM and its complications.
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- 2020
22. A 20-hydroxyecdysone-enriched fraction from Pfaffia glomerata (Spreng.) pedersen roots alleviates stress, anxiety, and depression in mice
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Letícia de Almeida Takata, Wagner Campos Otoni, Kristhiano Chagas, Veridiana de Melo Rodrigues Ávila, Rodrigo Rodrigues Franco, Luiz Ricardo Goulart, Cássia Regina Silva, Allisson Benatti Justino, André Luis Lopes Saraiva, and Foued Salmen Espindola
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Male ,Antioxidant ,medicine.medical_treatment ,Pharmacology ,Anxiety ,Motor Activity ,medicine.disease_cause ,Plant Roots ,Antioxidants ,Superoxide dismutase ,Lipid peroxidation ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Memory ,Drug Discovery ,medicine ,Animals ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Amaranthaceae ,biology ,Behavior, Animal ,Depression ,Plant Extracts ,Glutathione peroxidase ,Brain ,Glutathione ,Pfaffia glomerata ,biology.organism_classification ,Antidepressive Agents ,Mice, Inbred C57BL ,Disease Models, Animal ,Oxidative Stress ,Ecdysterone ,chemistry ,Anti-Anxiety Agents ,Catalase ,030220 oncology & carcinogenesis ,biology.protein ,Exploratory Behavior ,Lipid Peroxidation ,Oxidative stress ,Biomarkers ,Stress, Psychological - Abstract
Ethnopharmacological relevance Pfaffia glomerata roots are widely used in Brazil to treat various pathological conditions, particularly psychological disorders. 20-hydroxyecdysone, a phytosteroid present in the plant, can promote greater body resistance against exogenous and endogenous stressors. The objective of this study was to evaluate the possible neuroprotective effect of a 20-hydroxyecdysone-enriched fraction (20E-EF), obtained from P. glomerata roots, in an acute murine stress model. Material and methods The 20E-EF was obtained by partitioning the methanol extract from P. glomerata roots with dichloromethane. Mice were treated by gavage with three doses of 20E-EF (3, 10, and 30 mg/kg) and parameters of stress, anxiety, and depression were evaluated. Biomarkers of oxidative stress (enzymes, antioxidant profile, and oxidized molecules) were evaluated in the cortex, striatum (basal ganglia), and hippocampus of animals treated with 30 mg/kg of 20E-EF. Results Mass spectrometry revealed that 20E was the main compound in the dichloromethane fraction. At a dose of 30 mg/kg, 20E-EF reduced stress, anxiety, and depression, while stimulating antioxidant enzymes (catalase, superoxide dismutase, and glutathione peroxidase), promoting antioxidant activity (antioxidant capacity, sulfhydryl groups, and reduced glutathione), and reducing oxidative markers (lipid peroxidation). In addition, 20E increased the concentration of NO in the striatum, possibly improving memory function and antioxidant activity. Conclusion A 30 mg/kg dose of 20E-EF was able to reduce stress, anxiety, and depression, in addition to maintaining antioxidant defenses of the cortex and striatum. These findings open new perspectives for understanding the therapeutic properties of P. glomerata and the underlying mechanism(s).
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- 2020
23. Protective effects of a polyphenol-enriched fraction of the fruit peel of Annona crassiflora Mart. on acute and persistent inflammatory pain
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Priscilla Dias, Thiago Neves Vieira, André Luis Lopes Saraiva, Mônica Soares Costa, Allisson Benatti Justino, Paula Dechichi, Pedro Henrique Silva, Cássia Regina Silva, Camila Rodrigues Borges Linhares, Veridiana de Melo Rodrigues Ávila, and Foued Salmen Espindola
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0301 basic medicine ,Male ,Nociception ,Immunology ,Freund's Adjuvant ,Anti-Inflammatory Agents ,Arthritis ,Pain ,Inflammation ,Pharmacology ,Protective Agents ,Annona ,Antioxidants ,Nitric oxide ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Edema ,medicine ,Monoarthritis ,Animals ,Pharmacology (medical) ,Analgesics ,biology ,Plant Extracts ,Macrophages ,Annona crassiflora ,Polyphenols ,biology.organism_classification ,medicine.disease ,Mice, Inbred C57BL ,030104 developmental biology ,chemistry ,Hyperalgesia ,Fruit ,medicine.symptom ,030217 neurology & neurosurgery - Abstract
Different parts of Annona crassiflora Mart., a native species from Brazilian savanna, were traditionally used for the treatment of a wide variety of ailments including arthritis. Thus, this study aimed to investigate the possible antinociceptive and anti-inflammatory properties of a polyphenol-enriched fraction of the fruit peel of A. crassiflora, named here as EtOAc, in mice. Pro-inflammatory cytokines and nitric oxide (NO) production were evaluated in LPS-activated macrophages. Then, EtOAc fraction was administered by oral route in male C57BL/6/J mice, and the animals were submitted to glutamate-induced nociception and complete Freund's adjuvant (CFA)-induced monoarthritis tests to assess nociception (mechanical, spontaneous and cold pain) and inflammation (edema and neutrophil infiltration), and to the open-field and rotarod tests for motor performance analysis. EtOAc fraction inhibited the production of IL-6 and NO in the LPS-induced macrophages, and reduced spontaneous nociception induced by glutamate, without altering the animals' locomotor activity. In addition, the polyphenol-enriched fraction was able to revert the early and late hyperalgesia induced by CFA, as well as edema at the acute phase. Reduction of myeloperoxidase activity and inflammatory cell infiltration was observed in the paw tissue of mice injected with CFA and treated with EtOAc fraction. Together, our results support the antinociceptive and anti-inflammatory effects of the polyphenol-enriched fraction of A. crassiflora fruit peel and suggest that these effects are triggered, at least in part, by suppressing pro-inflammatory cytokines and neutrophils infiltration.
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- 2019
24. Annona muricata Linn. leaf as a source of antioxidant compounds with in vitro antidiabetic and inhibitory potential against α-amylase, α-glucosidase, lipase, non-enzymatic glycation and lipid peroxidation
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Allisson Benatti Justino, Foued Salmen Espindola, Neide M. Silva, Natália Carnevalli Miranda, Mário Machado Martins, and Rodrigo Rodrigues Franco
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Glycation End Products, Advanced ,Male ,0301 basic medicine ,Antioxidant ,DPPH ,medicine.medical_treatment ,Annona ,Antioxidants ,Lipid peroxidation ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Glycation ,medicine ,Animals ,Hypoglycemic Agents ,Food science ,Amylase ,Enzyme Inhibitors ,Rats, Wistar ,Annona muricata ,Pharmacology ,biology ,Plant Extracts ,Serum Albumin, Bovine ,alpha-Glucosidases ,Lipase ,General Medicine ,biology.organism_classification ,Plant Leaves ,030104 developmental biology ,chemistry ,Phytochemical ,030220 oncology & carcinogenesis ,NIH 3T3 Cells ,biology.protein ,Lipid Peroxidation ,Trolox ,alpha-Amylases - Abstract
Annona muricata leaves are used in traditional medicine to manage diabetes mellitus and its complications. The aim of this study was to evaluate the potential in vitro antidiabetic properties of Annona muricata leaf by identifying its main phytochemical constituents and characterizing the phenolic-enriched fractions for their in vitro antioxidant capacity and inhibitory activities against glycoside and lipid hydrolases, advanced glycation end-product formation and lipid peroxidation. Ethanol extract of A. muricata leaf was subjected to a liquid-liquid partitioning and its fractions were used in enzymatic assays to evaluate their inhibitory potential against α-amylase, α-glucosidase and lipase, as well as their antioxidant (DPPH, ORAC, FRAP and Fe2+-ascorbate-induced lipid peroxidation assays) and anti-glycation (BSA-fructose, BSA-methylglyoxal and arginine-methylglyoxal models) capacities. In addition, identification of the main bioactive compounds of A. muricata leaf by HPLC-ESI-MS/MS analysis was carried out. Ethyl acetate (EtOAc) and n-butanol (BuOH) fractions showed, respectively, antioxidant properties (ORAC 3964 ± 53 and 2707 ± 519 μmol trolox eq g−1, FRAP 705 ± 35 and 289 ± 18 μmol trolox eq g−1, and DPPH IC50 4.3 ± 0.7 and 9.3 ± 0.8 μg mL−1) and capacity to reduce liver lipid peroxidation (p
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- 2018
25. Antioxidant and anti-glycation capacities of some medicinal plants and their potential inhibitory against digestive enzymes related to type 2 diabetes mellitus
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Foued Salmen Espindola, Danúbia da Silva Carvalho, Francyelle Borges Rosa de Moura, Heitor Cappato Guerra Silva, Leonardo Gomes Peixoto, Rodrigo Rodrigues Franco, and Allisson Benatti Justino
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0301 basic medicine ,Antioxidant ,DPPH ,medicine.medical_treatment ,Antioxidants ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Bauhinia forficata ,Drug Discovery ,medicine ,Hypoglycemic Agents ,Glycoside Hydrolase Inhibitors ,Lipase ,Medicinal plants ,Pancreas ,IC50 ,Pharmacology ,Plants, Medicinal ,biology ,Traditional medicine ,Echinodorus grandiflorus ,alpha-Glucosidases ,Fructose ,biology.organism_classification ,030104 developmental biology ,Diabetes Mellitus, Type 2 ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,alpha-Amylases - Abstract
Ethnopharmacological relevance Plants preparations are used by traditional medicine in the treatment of various diseases, such as type-2 diabetes mellitus. Some medicinal plants are capable of controlling the complications of this metabolic disease at different levels, for example, providing antioxidant compounds that act against oxidative stress and protein glycation and others which are capable of inhibiting the catalysis of digestive enzymes and thus contribute to the reduction of hyperglycemia and hyperlipidemia. Our objective was to investigate the antioxidant and anti-glycation activities of some medicinal plants and their potential inhibitory against α-amylase, α-glucosidase and pancreatic lipase activities. Material and methods Based on the ethnobotanical researches carried out by academic studies conducted at the Federal University of Uberlandia, ten plants traditionally used in the treatment of type-2 diabetes mellitus were selected. Ethanol (EtOH) and hexane (Hex) extracts of specific parts of these plants were used in enzymatic assays to evaluate their inhibitory potential against α-amylase, α-glucosidase and lipase, as well as their antioxidant (DPPH, ORAC and FRAP) and anti-glycation (BSA/fructose model) capacities. Results The results indicate that EtOH extract of four of the ten analyzed plants exhibited more than 70% of antioxidant and anti-glycation capacities, and α-amylase and lipase inhibitory activities; no extract was able to inhibit more than 40% the α-glucosidase activity. The EtOH extracts of Bauhinia forficata and Syzygium. cumini inhibited α-amylase (IC50 8.17 ± 2.24 and 401.8 ± 14.7 μg/mL, respectively), whereas EtOH extracts of B. forficata, Chamomilla recutita and Echinodorus grandiflorus inhibited lipase (IC50 59.6 ± 10.8, 264.2 ± 87.2 and 115.8 ± 57.1 μg/mL, respectively). In addition, EtOH extracts of B. forficata, S. cumini, C. recutita and E. grandiflorus showed, respectively, higher antioxidant capacity (DPPH IC50 0.7 ± 0.1, 2.5 ± 0.2, 1.3 ± 0.2 and 35.3 ± 9.0 μg/mL) and anti-glycation activity (IC50 22.7 ± 4.4, 246.2 ± 81.7, 18.5 ± 2.8 and 339.0 ± 91.0 μg/mL). Conclusions EtOH extracts of four of the ten species popularly cited for treatment of type 2 diabetes mellitus have shown promising antioxidant and anti-glycation properties, as well as the ability to inhibit the digestive enzymes α-amylase and lipase. Thus, our results open new possibilities for further studies in order to evaluate the antidiabetic potential of these medicinal plants.
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- 2018
26. Hepatoprotective Properties of a Polyphenol-Enriched Fraction from Annona crassiflora Mart. Fruit Peel against Diabetes-Induced Oxidative and Nitrosative Stress
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Renata Roland Teixeira, Francyelle Borges Rosa de Moura, Heitor Cappato Guerra Silva, Leonardo Gomes Peixoto, Izabela Barbosa Moraes, Foued Salmen Espindola, Mariana Nunes Pereira, Douglas Carvalho Caixeta, Adriele Vieira de Souza, Allisson Benatti Justino, and Danielle Diniz Vilela
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Male ,0301 basic medicine ,Antioxidant ,medicine.medical_treatment ,Protective Agents ,medicine.disease_cause ,Annona ,Antioxidants ,Diabetes Mellitus, Experimental ,Superoxide dismutase ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Animals ,Humans ,Aspartate Aminotransferases ,Procyanidin B2 ,chemistry.chemical_classification ,Glutathione Peroxidase ,Molecular Structure ,biology ,Plant Extracts ,Chemistry ,Glutathione peroxidase ,Polyphenols ,Alanine Transaminase ,Catechin ,General Chemistry ,Glutathione ,Rats ,Oxidative Stress ,030104 developmental biology ,Liver ,Biochemistry ,Catalase ,Fruit ,030220 oncology & carcinogenesis ,biology.protein ,General Agricultural and Biological Sciences ,Oxidative stress - Abstract
A polyphenol-enriched fraction from Annona crassiflora fruit peel (Ac-Pef) containing chlorogenic acid, (epi)catechin, procyanidin B2, and caffeoyl-glucoside was investigated against hepatic oxidative and nitrosative stress in streptozotocin-induced diabetic rats. Serum biochemical parameters, hepatic oxidative and nitrosative status, glutathione defense system analysis, and in silico assessment of absorption, distribution, metabolism, excretion, and toxicity (ADMET) of the main compounds of Ac-Pef were carried out. Ac-Pef treatment during 30 days decreased serum alanine aminotransferase, aspartate aminotransferase, and alkaline phosphatase activities, as well as hepatic lipid peroxidation, protein carbonylation and nitration, inducible nitric oxide synthase level, and activities and expressions of glutathione peroxidase, superoxide dismutase, and catalase. There were increases in antioxidant capacity, glutathione reductase activity, and reduced glutathione level. ADMET predictions of Ac-Pef compounds showed favorable absorption and distribution, with no hepatotoxicity. A. crassiflora fruit peel showed hepatoprotective properties, indicating a promising natural source of bioactive molecules for prevention and therapy of diabetes complications.
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- 2017
27. Salivary and Plasmatic Antioxidant Profile following Continuous, Resistance, and High-Intensity Interval Exercise: Preliminary Study
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Adriele Vieira de Souza, Jéssica S Giolo, Douglas Carvalho Caixeta, Leonardo Gomes Peixoto, Foued Salmen Espindola, Renata Roland Teixeira, Guilherme Morais Puga, Danielle Diniz Vilela, and Allisson Benatti Justino
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Adult ,Male ,Aging ,medicine.medical_specialty ,Saliva ,Antioxidant ,Sports medicine ,Article Subject ,medicine.medical_treatment ,Physical exercise ,Biochemistry ,Antioxidants ,Nitric oxide ,03 medical and health sciences ,chemistry.chemical_compound ,Young Adult ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Exercise physiology ,lcsh:QH573-671 ,Exercise ,business.industry ,Superoxide Dismutase ,lcsh:Cytology ,030229 sport sciences ,Cell Biology ,General Medicine ,Blood Proteins ,Catalase ,Glutathione ,Uric Acid ,Oxidative Stress ,Endocrinology ,chemistry ,Exercise intensity ,Uric acid ,business ,Oxidation-Reduction ,030217 neurology & neurosurgery ,Biomarkers ,Research Article - Abstract
The increase in antioxidant responses promoted by regular physical activity is strongly associated with the attenuation of chronic oxidative stress and physiological mechanisms related to exercise adaptation. The aim of this work was to evaluate and compare how different exercise protocols (HIIE: high-intensity interval exercise, CE: continuous exercise, and RE: resistance exercise) may alter salivary and plasmatic antioxidants and salivary markers of exercise intensity and nitric oxide. Thirteen healthy, trained male subjects were submitted to the three exercise protocols. Blood and saliva samples were collected at the points preexercise, postexercise, and 3 hours postexercise. Antioxidants (total antioxidant capacity, superoxide dismutase and catalase activities, and levels of reduced glutathione and uric acid), markers of exercise intensity (salivary total protein and amylase activity), and salivary nitric oxide were evaluated. As a result, all exercise protocols increased the markers of exercise intensity and nitric oxide. Antioxidant response was increased after exercise, and it was found that a single HIIE session exerts a similar pattern of antioxidant response compared to CE, in plasma and saliva samples, while RE presented minor alterations. We suggest that HIIE may lead to alterations in antioxidants and consequently to the physiological processes related to redox, similar to the CE, with the advantage of being performed in a shorter time. In addition, the antioxidant profile of saliva samples showed to be very similar to that of plasma, suggesting that saliva may be an alternative and noninvasive tool in sports medicine for the study of antioxidants in different physical exercise protocols.
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- 2019
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28. Potential Benefits of Nitrate Supplementation on Antioxidant Defense System and Blood Pressure Responses after Exercise Performance
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Renata Roland Teixeira, Guilherme Morais Puga, Foued Salmen Espindola, Eduardo F Menezes, Allisson Benatti Justino, and Leonardo Gomes Peixoto
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Adult ,Male ,0301 basic medicine ,Aging ,Saliva ,Antioxidant ,Article Subject ,medicine.medical_treatment ,Blood Pressure ,Biochemistry ,Antioxidants ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Animal science ,medicine ,TBARS ,Humans ,Aerobic exercise ,Exercise physiology ,lcsh:QH573-671 ,Exercise ,Nitrites ,Cross-Over Studies ,Nitrates ,030109 nutrition & dietetics ,business.industry ,lcsh:Cytology ,030229 sport sciences ,Cell Biology ,General Medicine ,Crossover study ,Oxidative Stress ,Blood pressure ,chemistry ,Dietary Supplements ,Exercise Test ,Uric acid ,business ,Research Article - Abstract
Nitrate (NO3−) supplementation is associated with exercise performance, oxygen uptake, blood flow, and blood pressure improvement, and it can act as an antioxidant agent. This study evaluated the effects of sodium nitrate supplementation on oxidative stress markers and blood pressure responses after aerobic exercise performance in physically active males. Fourteen subjects aged 22±3 years and with a BMI of 23±1 kg/m2 were submitted to four exercise tests in intervals of 5 days. Nitrate supplementation (NO session) and placebo supplementation (PL session) were acute (AC) and over a period of 5 days (FD) in random order with a crossover design. Saliva was collected at basal (0′); 60 min after supplementation (60′); immediately after exercise (90′); and 15, 30, and 60 min after the test (105′, 120′, and 150′). The NO session had higher concentrations (P<0.05) of salivary nitrite in both AC and FD treatments when compared with the PL session. There was a reduction in systolic blood pressure (SBP) only after FD in the NO session. Furthermore, uric acid and total antioxidant capacity (FRAP) salivary concentrations increased, while SOD activity and TBARS levels decreased after FD but not after AC in the NO session. The results suggest that nitrate supplemented over a period of 5 days reduced SBP and indirectly acted as an antioxidant in healthy nonsedentary young men.
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- 2019
29. Phytoscreening of Vochysiaceae species: Molecular identification by HPLC-ESI-MS/MS and evaluating of their antioxidant activity and inhibitory potential against human α-amylase and protein glycation
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Foued Salmen Espindola, Prv Campana, Mário Machado Martins, V.L. De Almeida, Rodrigo Rodrigues Franco, Júlio César Dias Lopes, Catarina Silva, and Allisson Benatti Justino
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Glycation End Products, Advanced ,Antioxidant ,DPPH ,medicine.medical_treatment ,Phytochemicals ,Drug Evaluation, Preclinical ,01 natural sciences ,Biochemistry ,Antioxidants ,chemistry.chemical_compound ,Vochysiaceae ,Drug Discovery ,medicine ,Humans ,Hypoglycemic Agents ,Glycoside Hydrolase Inhibitors ,Food science ,Molecular Biology ,Enzyme Assays ,chemistry.chemical_classification ,biology ,010405 organic chemistry ,Organic Chemistry ,Methylglyoxal ,Myrtales ,Glycoside ,Lipase ,biology.organism_classification ,0104 chemical sciences ,Plant Leaves ,010404 medicinal & biomolecular chemistry ,Proanthocyanidin ,chemistry ,Polyphenol ,Plant Bark ,Trolox ,alpha-Amylases - Abstract
Scientific research based on medicinal plants has been highlighted as a complementary treatment to T2DM, stand out the Vochysiaceae family, which have been widely used in folk medicine by traditional South American communities to treat some diseases. Our study aimed to investigate the antioxidant and antiglycation activities of ethanol extracts of leaves (LF) and stem barks (SB) of Vochysiaceae species, evaluated their capacities to inhibit glycoside and lipid hydrolases related to T2DM and molecular identification by HPLC-ESI-MS/MS. Our main findings indicate that the ethanolic extract of four of eight analyzed plants such as LF and SB of Q. grandiflora, Q. parviflora, V. elliptica and Calisthene major exhibited, respectively, potential of α-amylase inhibition (IC50 of LF: 5.7 ± 0.6, 4.1 ± 0.5, 5.8 ± 0.5, 3.2 ± 0.6 and IC50 of SB: 3.3 ± 0.7, 6.2 ± 2.0, 121.0 ± 8.6 and 11.2 ± 2.8 μg/mL), capacities of antioxidant (ORAC of LF: 516.2 ± 0.1, 547.6 ± 4.9, 544.3 ± 6.1, 442.6 ± 2.4 and ORAC of SB: 593.6 ± 22.3, 497.7 ± 0.8, 578 ± 12.3, 593.6 ± 19.5 µmol trolox eq/g; FRAP of LF: 796.1 ± 0.9, 427.7 ± 22.0, 81.0 ± 1.9, 685 ± 37.9 and FRAP of SB: 947.4 ± 24.9, 738.6 ± 24.3, 98.8 ± 7.9, 970.8 ± 13.9 µmol trolox eq/g; DPPH IC50 of LF: 14.2 ± 1.8, 36.3 ± 6.9, 11.8 ± 1.9, 13.3 ± 1.2 and DPPH IC50 of SB: 16.0 ± 3.0, 15.5 ± 1.9, 126.1 ± 23. 6, 5.3 ± 0.3 μg/mL, respectively) and antiglycation (BSA/Frutose IC50 of LF: 43.1 ± 3.4, 52.1 ± 6.0, 175.5 ± 32, 8, 111.8 ± 14.7 and BSA/Frutose IC50 of SB:, 40.1 ± 11.9, 51.2 ± 16. 7, 46.6 ± 5.7, 53.5 ± 13.6 μg/mL) and presence of polyphenols, such as flavonoids and condensed tannins. The extracts presented low ability to inhibit α-glycosidase and lipase enzymes in the initial assays, with values below 40% of inhibition. In BSA/methylglyoxal, only Q. grandiflora SB, V. eliptica LF and V. tucanorum LF showed activity (IC50: 655.5 ± 208.5, 401.9 ± 135.2 and 617.1 ± 80.6 μg/mL, respectively) and only C. major LF and SB, in Arg/methylglyoxal (IC50: 485.1 ± 130.8 and 468.0 ± 150.5 μg/ml, respectively). This study presented new findings about the biological and pharmacological potential of some species of Vochysiaceae family, contributing to the understanding of the action and efficacy in use of these plants, in their management of postprandial hyperglycemia and in glycation and oxidative processes that contribute to managing diabetes mellitus.
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- 2018
30. Antioxidant compounds from Banisteriopsis argyrophylla leaves as α-amylase, α-glucosidase, lipase, and glycation inhibitors
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Mário Machado Martins, Daiane M.O. Quaresma, Rodrigo A.A. Munoz, Marcos Pivatto, Francisco José Tôrres de Aquino, Alberto de Oliveira, Allisson Benatti Justino, Foued Salmen Espindola, Raquel M. F. Sousa, and Luiz Ricardo Goulart
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Glycosylation ,Antioxidant ,Oxygen radical absorbance capacity ,DPPH ,medicine.medical_treatment ,Drug Evaluation, Preclinical ,01 natural sciences ,Biochemistry ,Antioxidants ,Catechin ,chemistry.chemical_compound ,Glucosides ,Glycation ,Drug Discovery ,medicine ,Humans ,Hypoglycemic Agents ,Proanthocyanidins ,Enzyme Inhibitors ,Kaempferols ,Lipase ,Molecular Biology ,Flavonoids ,Chromatography ,biology ,Cyclohexanones ,Plant Extracts ,010405 organic chemistry ,Banisteriopsis ,Organic Chemistry ,alpha-Glucosidases ,0104 chemical sciences ,Plant Leaves ,010404 medicinal & biomolecular chemistry ,chemistry ,biology.protein ,Trolox ,alpha-Amylases ,Norisoprenoids ,Kaempferol - Abstract
Banisteriopsis argyrophylla belongs to the Malpighiaceae family, which is a species from Cerrado, also known as “cipo-prata” or “cipo-folha-de-prata.” Several species of this family present biological potential. This work reports the chemical identification of the ethanol extract (EE) and its fractions from B. argyrophylla leaves and shows the analysis of the antioxidant activity and inhibitory effects on activities of α-amylase, α-glucosidase and lipase, and non-enzymatic glycation. The ethyl acetate fraction (EAF) and n-butanol fraction (BF) showed antioxidant activity, with IC50 values of 4.1 ± 0.1 and 4.8 ± 0.1 μg mL−1, respectively, by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, and IC50 values of 6046.3 ± 174.2 and 6264.2 ± 32.2 µmol Trolox eq g−1 by the oxygen radical absorbance capacity (ORAC) method. Furthermore, the DPPH method with these fractions presented electroactive species with antioxidant potential, as shown by the differential pulse voltammetry (DPV) method. The inhibitory effects of the EAF and BF were demonstrated by the following results: IC50 of 5.1 ± 0.3 and 2.5 ± 0.2 μg mL−1 for α-amylase, IC50 of 1093.5 ± 26.0 and 1250.8 ± 21.9 μg mL−1 for α-glucosidase, IC50 of 8.3 ± 4.1 and 4.4 ± 1.0 μg mL−1 for lipase, and IC50 of 1.3 ± 0.1 and 0.9 ± 0.1 μg mL−1 for glycation. Some bioactive compounds were identified by (–)-ESI-MS/MS, such as catechin, procyanidins, glycosylated flavonoids, kaempferol, and megastigmane glucosides. The antidiabetic activity of B. argyrophylla has been reported for the first time.
- Published
- 2020
31. Antidiabetic effects of Syzygium cumini leaves: A non-hemolytic plant with potential against process of oxidation, glycation, inflammation and digestive enzymes catalysis
- Author
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Allisson Benatti Justino, Rodrigo Rodrigues Franco, Joed Pires de Lima Júnior, Luiz Fernando Ribeiro Zabisky, Victor Hugo Mota Alves, André Lopes Saraiva, Luiz Ricardo Goulart, and Foued Salmen Espindola
- Subjects
Glycation End Products, Advanced ,Male ,Spectrometry, Mass, Electrospray Ionization ,Antioxidant ,DPPH ,Syzygium ,medicine.medical_treatment ,Flavonoid ,Anti-Inflammatory Agents ,Antioxidants ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Tandem Mass Spectrometry ,Drug Discovery ,medicine ,Animals ,Hypoglycemic Agents ,Glycoside Hydrolase Inhibitors ,Enzyme Inhibitors ,Rats, Wistar ,Chromatography, High Pressure Liquid ,030304 developmental biology ,Pharmacology ,chemistry.chemical_classification ,0303 health sciences ,Traditional medicine ,Plant Extracts ,Methylglyoxal ,Catechin ,Lipase ,Mice, Inbred C57BL ,Plant Leaves ,chemistry ,030220 oncology & carcinogenesis ,Digestion ,Lipid Peroxidation ,Trolox ,alpha-Amylases ,Quercetin ,Kaempferol ,Oxidation-Reduction - Abstract
Ethnopharmacological relevance Plant materials are commonly used in traditional medicine in order to treat various diseases such as Diabetes mellitus. Some plants, such as Syzygium cumini, have the capability to act controlling oxidative stress and protein glycation besides their potential to decrease hyperglycemia and hyperlipidemia by the inhibition of the catalysis of digestive enzymes. The aim of this study was to evaluate the antioxidant and antiglicant activity of S. cumini leaves fractions, their capacity to inhibit hydrolases and lipase enzymes, as well as the cytotoxicity effects against erythrocytes and comparate these results with isolate quercetin flavonoid. Material and methods Ethnobotanical researches, carried out by academic studies at the Federal University of Uberlandia, led us to choose S. cumini as a potential plant for treatment of Diabetes mellitus. Fractions from ethanolic extract of S. cumini (hexane/Hex, dichloromethane/DCM, ethyl acetate/EtOAc, n-butanol/ButOH and water/H2O) were used to evaluate their antioxidant (DPPH, ORAC and FRAP) and antiglycant (BSA/fructose, BSA/methylglyoxal and Arginine/Methylglyoxal) activity as well as the inhibitory potential against α-amylase, α-glucosidase and lipase. In addition, identification of the main bioactive compounds of S. cuimini leaves by HPLC-ESIMS/MS analysis was carried out. Results Our results indicate that all fractions, for exception Hex, present noteworthy antioxidant activity, mainly in EtOAc and ButOH fractions (FRAP 1154.49 ± 67.37 and 1178.27 ± 21.26 μmol trolox eq g−1, respectively; ORAC 1224.63 ± 58.16 and 1313.53 ± 85.23 μmol trolox eq g−1, respectively; DPPH IC50 15.7 ± 2.4 and 23.5 ± 2.7 μg mL−1, respectively). Regarding the antiglycant activity (BSA/fructose and Arginine/Methylglyoxal models), all fraction, for exception Hex, presented inhibition higher than 85%. All fractions were capable to inhibit 100% of α-amylase and the fractions DCM, EtOAc and ButOH inhibited α-glucosidase more than 50%. Regarding the lipase assay, DCM and Hex had the best activity (31.5 ± 14.3 and 44.3 ± 4.5 μg mL−1, respectively). Various biomolecules known as potent antioxidants were identified in these fractions, such as quercetin, kaempferol, luteolin and (Epi)catechin. Conclusion S. cumini fractions and quercetin presented promising antioxidant and antiglycation properties as well as the ability to inhibit digestive enzymes. This study presents new biological activities not yet described for S. cumini which provide new possibilities for further studies in order to assess the antidiabetic potential of S. cumini fractions especially EtOAc and ButOH.
- Published
- 2020
32. 5α-Dihydrotestosterone enhances wound healing in diabetic rats
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Rômulo Dias Novaes, Mariáurea Matias Sarandy, Neire Moura de Gouveia, Reggiani Vilela Gonçalves, Sérgio Luis Pinto da Matta, Foued Salmen Espindola, Eduardo Medeiros Damasceno, and Mariella Bontempo Freitas
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,Drug Compounding ,medicine.medical_treatment ,Intraperitoneal injection ,Silver sulfadiazine ,Antioxidants ,Collagen Type I ,General Biochemistry, Genetics and Molecular Biology ,Diabetes Mellitus, Experimental ,Protein Carbonylation ,Cicatrix ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Mast Cells ,Rats, Wistar ,General Pharmacology, Toxicology and Pharmaceutics ,Saline ,Testosterone ,Skin ,Wound Healing ,business.industry ,Lanolin ,Dihydrotestosterone ,General Medicine ,Streptozotocin ,Rats ,Surgery ,Collagen Type III ,030104 developmental biology ,Endocrinology ,business ,Wound healing ,medicine.drug - Abstract
Wound healing involves a complex interaction between the cells, extracellular matrix and oxidative response. Aims Analyze the effects of 5α-Dihydrotestosterone (5α-DTH) ointment in cutaneous wound healing by secondary intention in diabetic Wistar rats. Main methods Rats (302.23 ± 26.23 g, n = 48) were maintained in cages with food and water ad libitum in accordance with the Guiding Principles in the Use of Animal Ethics Committee. Diabetes was induced by intraperitoneal injection of streptozotocin (60 mg/kg). Three skin wounds (12 mm diameter) were created on the animals' back, which were randomized into 6 groups according to the application received: VT group: Vehicle (lanolin), SA group: 0.9% saline solution, NC group: Non-diabetic, CP group: positive control (silver sulfadiazine 0001%), T1 group: Testosterone (10%), T2 group: Testosterone (20%) emulsified in lanolin. The applications were made daily within 21 days, and tissues from different wounds were removed every 7 days. Key findings Both groups treated with testosterone (T1 and T2) showed a significantly higher proportion of type I and type III collagen fibers. Superoxide dismutase levels were significantly higher on days 7 and 14 in testosterone treated groups. Protein carbonyls and MDA were lower in both groups. Significance We conclude that groups treated with 5α-DTH showed a better healing pattern with complete wound closure, and proved to have a positive effect on the morphology of the scar tissue as well as an antioxidant stimulating effect during secondhand intention skin wounds repair in diabetic rats.
- Published
- 2016
33. Antidiabetic potential of Bauhinia forficata Link leaves: a non-cytotoxic source of lipase and glycoside hydrolases inhibitors and molecules with antioxidant and antiglycation properties
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Luiz Fernando Ribeiro Zabisky, André Lopes Saraiva, Victor Hugo Mota Alves, Allisson Benatti Justino, Luiz Ricardo Goulart, Mário Machado Martins, Foued Salmen Espindola, and Rodrigo Rodrigues Franco
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Male ,0301 basic medicine ,Glycosylation ,Antioxidant ,medicine.medical_treatment ,Phytochemicals ,medicine.disease_cause ,Antioxidants ,chemistry.chemical_compound ,Diabetes mellitus ,0302 clinical medicine ,chemistry.chemical_classification ,biology ,Traditional medicine ,General Medicine ,Phytochemical ,Bauhinia ,030220 oncology & carcinogenesis ,Cell Survival ,RM1-950 ,Natural compounds ,Hemolysis ,03 medical and health sciences ,Bauhinia forficata ,medicine ,Kaempferitrin ,Animals ,Hypoglycemic Agents ,Glycoside Hydrolase Inhibitors ,Rats, Wistar ,Lipase ,Pharmacology ,Mass spectrometry ,Plant Extracts ,Polyphenols ,alpha-Glucosidases ,biology.organism_classification ,Mice, Inbred C57BL ,Plant Leaves ,Kinetics ,030104 developmental biology ,Enzyme ,chemistry ,Oxidative stress ,Polyphenol ,biology.protein ,Therapeutics. Pharmacology ,alpha-Amylases ,Reactive Oxygen Species - Abstract
Bauhinia forficata Link., a cerrado native plant, is used as a complementary treatment for Type 2 Diabetes Mellitus (T2DM). Several studies involving this plant have shown that it has prominent potential to combat hyperglycemia and oxidative stress. Our objective was suggest the phytochemical constitution of fractions of ethanol extract of B. forficata leaves using HPLC-ESI-MS/MS, and evaluates their activities in enzymatic assays to evaluate their inhibitory potential against α-amylase, α-glucosidase and lipase, as well as their antioxidant and anti-glycation capacities. In addition, we evaluated the cytotoxic effects of these fractions using rodents macrophages and erythrocytes. The ETOAC e ButOH fractions showed high polyphenols concentrations, having been determined 11 flavonoids, including the kaempferitrin, the phytomarker of B. forficata Link. In addition, all fractions presented higher antioxidant and antiglycation activities and prominent capacities to digestive enzymes inhibition. On the other hand, in the cellular assays, none fractions showed cytotoxic and hemolytic effects, able to combat the ROS production in macrophages. Thus, this study presented new results on the biological activities of this plant, contributing to the understanding of the action and effectiveness of its use in the management of diabetes mellitus and its complications.
- Published
- 2020
34. Interdisciplinary therapy changes superoxide dismutase activity and adiponectin in obese adolescents: a randomised controlled trial
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Heitor Santos Cunha, Ana Raimunda Dâmaso, Nadia Carla Cheik, Foued Salmen Espindola, João Elias Dias Nunes, Zulmária Zulmária Rezende Ramos de Freitas, and Ana Maria Oliveira Caixeta Nogueira
- Subjects
Counseling ,Male ,0301 basic medicine ,Pediatric Obesity ,medicine.medical_specialty ,Adolescent ,medicine.medical_treatment ,030209 endocrinology & metabolism ,Physical Therapy, Sports Therapy and Rehabilitation ,Superoxide dismutase activity ,medicine.disease_cause ,Antioxidants ,law.invention ,Superoxide dismutase ,Group psychotherapy ,03 medical and health sciences ,0302 clinical medicine ,Randomized controlled trial ,law ,medicine ,Humans ,Orthopedics and Sports Medicine ,Exercise ,Health Education ,Referral and Consultation ,Nitrites ,Inflammation ,Adiponectin ,biology ,Superoxide Dismutase ,business.industry ,medicine.disease ,Obesity ,Diet ,Oxidative Stress ,030104 developmental biology ,Psychotherapy, Group ,Physical therapy ,biology.protein ,Female ,business ,Blood parameters ,Oxidative stress - Abstract
The objective of this study is to evaluate the effect of interdisciplinary therapy in the parameters of the oxidative stress and the anti-inflammatory responses of obese adolescents. We selected 57 participants, who were randomly divided into 2 groups: interdisciplinary therapy group and a control group. After 6 months of intervention, 17 participants of the interdisciplinary therapy group and 8 of the control group returned for re-evaluation. The interdisciplinary therapy group participated in a treatment with 4 weekly sessions of exercise, a weekly group therapy session and a weekly nutritional education session. Blood parameters of oxidative stress and anti-inflammatory response were evaluated. The results demonstrated that there were significant increases in the interdisciplinary therapy group for superoxide dismutase activity (6.56 ± 3.22 to 11.40 ± 7.49) and ferric-reducing antioxidant potential concentration (532.91 ± 106.48 to 573.25 ± 112.57), although adiponectin levels did not reduce (40.9 ± 29.34 to 49.05 ± 41.22). A significant decrease in nitrite levels was also found (14.23 ± 8.48 to 11.45 ± 6.05). In the control group, significant reduction was found in adiponectin (31.56 ± 18.88 to 18.01 ± 11.66). This study suggests that interdisciplinary therapy for 6 months was effective in improving the anti-inflammatory responses and the antioxidant defences in obese adolescents.
- Published
- 2015
35. Royal jelly decreases corticosterone levels and improves the brain antioxidant system in restraint and cold stressed rats
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Helen Lara Machado, Leonardo Gomes Peixoto, Renata Roland Teixeira, Celso Rodrigues Franci, Foued Salmen Espindola, Douglas Carvalho Caixeta, Nathalia Belele Baptista, Danielle Diniz Vilela, and Adriele Vieira de Souza
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Male ,Restraint, Physical ,0301 basic medicine ,medicine.medical_specialty ,Central nervous system ,Hippocampus ,Striatum ,Biology ,Neuroprotection ,Antioxidants ,Lipid peroxidation ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Corticosterone ,Internal medicine ,medicine ,Animals ,Rats, Wistar ,Cerebral Cortex ,Adrenal gland ,General Neuroscience ,Fatty Acids ,Brain ,GELEIA REAL ,Corpus Striatum ,Cold Temperature ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,nervous system ,chemistry ,Cerebral cortex ,Stress, Psychological ,030217 neurology & neurosurgery - Abstract
Restraint and cold stress induces the hypothalamic-pituitary-adrenal (HPA) axis to release corticosterone from the adrenal gland, which can worsen the antioxidant defense system in the central nervous system. Here, we investigated the corticosterone levels and the antioxidant defense system in the cerebellum and brain, as well as in its isolated regions, such as cerebral cortex, striatum and hippocampus of stressed rats supplemented with royal jelly (RJ). Wistar rats were supplemented with RJ for 14days and the stress induction started on the 7th day. Stressed rats increased corticosterone levels, glycemia and lipid peroxidation in the brain and cerebellum, cerebral cortex and hippocampus besides reduced glutathione defense system in the brain and striatum. Rats supplemented with RJ decreased corticosterone, maintained glycemia and decreased lipid peroxidation in the brain, cerebellum, as well as striatum and hippocampus, besides improved glutathione defense system in cerebral cortex and striatum. This study suggests an anti-stress and neuroprotective effect of RJ under stress conditions.
- Published
- 2017
36. Phytochemical characterization of the Vochysia rufa (Vochysiaceae) extract and its effects on oxidative stress in the pancreata of streptozotocin-induced diabetic rats
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Foued Salmen Espindola, Camila Botelho Miguel, Carlo José Freire Oliveira, Luciana Karen Calábria, Alberto de Oliveira, Wellington Francisco Rodrigues, Raquel M. F. Sousa, Javier Emilio Lazo-Chica, João Henrique G. Lago, Antonio Vicente Mundim, Vagner Bezerra dos Santos, Neire Moura de Gouveia, and Claudimir Lucio do Lago
- Subjects
Blood Glucose ,Male ,0301 basic medicine ,Antioxidant ,Physiology ,Thiobarbituric acid ,medicine.medical_treatment ,lcsh:Medicine ,Biochemistry ,Antioxidants ,Glibenclamide ,chemistry.chemical_compound ,Endocrinology ,0302 clinical medicine ,Medicine and Health Sciences ,Urea ,Insulin ,lcsh:Science ,chemistry.chemical_classification ,Multidisciplinary ,Organic Compounds ,Glutathione peroxidase ,Catalase ,Glutathione ,Blood Sugar ,Body Fluids ,Chemistry ,Blood ,Physiological Parameters ,030220 oncology & carcinogenesis ,Physical Sciences ,Anatomy ,Research Article ,medicine.drug ,medicine.medical_specialty ,Endocrine Disorders ,Blood sugar ,Endocrine System ,Thiobarbituric Acid Reactive Substances ,Diabetes Mellitus, Experimental ,FITOQUÍMICA ,Magnoliopsida ,03 medical and health sciences ,Exocrine Glands ,Internal medicine ,Diabetes Mellitus ,medicine ,Animals ,Rats, Wistar ,Pancreas ,Diabetic Endocrinology ,Plant Extracts ,Superoxide Dismutase ,lcsh:R ,Body Weight ,Organic Chemistry ,Chemical Compounds ,Biology and Life Sciences ,Cell Biology ,Streptozotocin ,Hormones ,Rats ,Oxidative Stress ,030104 developmental biology ,chemistry ,Metabolic Disorders ,lcsh:Q ,Phytotherapy - Abstract
Aqueous extract of macerated Vochysia rufa stem bark has been commonly used in the treatment of diabetes. Therefore, we evaluated the antihyperglycemic and antioxidant effects of an extract of V. rufa on the pancreata of streptozotocin (STZ)-induced diabetic rats. Animals received one of the following treatments daily by oral gavage: water (diabetic-control), V. rufa extract (diabetic-V. rufa), or glibenclamide (diabetic-GBD). Total antioxidant capacity; levels of thiobarbituric acid reactive substances, reduced glutathione, and sulfhydryls; and superoxide dismutase, catalase, and glutathione peroxidase (GPx) activities were measured in the pancreas. Biochemical analysis of serum total cholesterol and fractions, triglycerides, creatinine, urea, acid uric, ALP, γ-GT, AST, and ALT was performed, and pancreatic β-cells positive for insulin were evaluated by immunohistochemistry. Rats treated with extract exhibited a decrease in fasting blood glucose compared with levels in diabetic control rats. GPx activity and sulfhydryl levels were significantly lower in diabetic-V. rufa rats compared with those of diabetic-control rats. V. rufa extract acted to normalize the biochemical alterations found in diabetic rats (diabetic-controls), as demonstrated by increases in urea, HDL, ALP, AST, and ALT. Reduction in blood glucose was independent of an increase in insulin. The V. rufa extract was found to be composed of free sugars (inositol, galactose, glucose, mannose, sucrose, arabinose, and ribose) as the main metabolites. Thus, aqueous extract of the stem bark of V. rufa is capable of reducing blood glucose, resulting in an antioxidant effect on the pancreatic tissue of STZ-diabetic rats.
- Published
- 2017
37. Neuroprotective effects of Pouteria ramiflora (Mart.) Radlk (Sapotaceae) extract on the brains of rats with streptozotocin-induced diabetes
- Author
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Luciana Karen Calábria, Renato Oliveira, Marcelo Emílio Beletti, Alice Vieira da Costa, Neire Moura de Gouveia, Vanessa Neves de Oliveira, Fabiana Barcelos Furtado, and Foued Salmen Espindola
- Subjects
Blood Glucose ,Male ,medicine.medical_specialty ,Antioxidant ,medicine.medical_treatment ,Blotting, Western ,Myosin Type V ,Hippocampus ,Biochemistry ,Neuroprotection ,Antioxidants ,Diabetes Mellitus, Experimental ,Pouteria ,Lipid peroxidation ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,Internal medicine ,Diabetes mellitus ,medicine ,Animals ,Rats, Wistar ,Acarbose ,chemistry.chemical_classification ,Reactive oxygen species ,biology ,Plant Extracts ,Superoxide Dismutase ,business.industry ,Pyramidal Cells ,Body Weight ,Brain ,medicine.disease ,biology.organism_classification ,Streptozotocin ,Immunohistochemistry ,Sapotaceae ,Rats ,Plant Leaves ,Oxidative Stress ,Neuroprotective Agents ,Endocrinology ,chemistry ,Lipid Peroxidation ,Neurology (clinical) ,business ,medicine.drug - Abstract
Diabetes mellitus is a chronic disease involving persistent hyperglycemia, which causes an imbalance between reactive oxygen species and antioxidant enzymes and results in damage to various tissues, including the brain. Many societies have traditionally employed medicinal plants to control the hyperglycemia. Pouteria ramiflora, a species occurring in the savanna biome of the Cerrado (Brazil) has been studied because of its possible ability to inhibit carbohydrate digestion. Rats with streptozotocin-induced diabetes treated with an alcoholic extract of Pouteria ramiflora show an improved glycemic level, increased glutathione peroxidase activity, decreased superoxide dismutase activity, and reduced lipid peroxidation and antioxidant status. The extract also restored myosin-Va expression and the nuclear diameters of pyramidal neurons of the CA3 subregion and that of the polymorphic cells of the hilus. We conclude that Pouteria ramiflora extract exerts a neuroprotective effect against oxidative damage and myosin-Va expression and is able to prevent hippocampal neuronal loss in the CA3 and hilus subfields of diabetic rats. However, future studies are needed to understand the mechanism of action of Pouteria ramiflora extract in acute and chronic diabetes.
- Published
- 2013
38. Peel of araticum fruit (Annona crassiflora Mart.) as a source of antioxidant compounds with α-amylase, α-glucosidase and glycation inhibitory activities
- Author
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Raquel M. F. Sousa, Foued Salmen Espindola, Alberto de Oliveira, Danielle Diniz Vilela, Natália Carnevalli Miranda, Neide M. Silva, Mário Machado Martins, Leonardo Gomes Peixoto, Renata Roland Teixeira, Mariana Nunes Pereira, and Allisson Benatti Justino
- Subjects
0301 basic medicine ,DPPH ,Cell Survival ,01 natural sciences ,Biochemistry ,Annona ,Antioxidants ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,Structure-Activity Relationship ,Nutraceutical ,Chlorogenic acid ,Glycation ,Drug Discovery ,Animals ,Food science ,Enzyme Inhibitors ,Molecular Biology ,biology ,Dose-Response Relationship, Drug ,010401 analytical chemistry ,Organic Chemistry ,Annona crassiflora ,Catechin ,alpha-Glucosidases ,Fibroblasts ,biology.organism_classification ,0104 chemical sciences ,030104 developmental biology ,chemistry ,Annonaceae ,Fruit ,NIH 3T3 Cells ,alpha-Amylases ,Kaempferol - Abstract
Annona crassiflora Mart., whose fruit is popularly known as araticum, is a member of the Annonaceae family found in the Brazilian Cerrado. Although this plant has several medicinal uses, its bioactive molecules are not fully understood. A bioguided assay was performed to identify the main bioactive compounds of A. crassiflora fruit peel from the ethanol extract fractions with antioxidant capacity and α-amylase, α-glucosidase and glycation inhibitory activities. Ethyl acetate and n-butanol fractions showed, respectively, higher antioxidant capacity (DPPH IC50 1.5±0.1 and 0.8±0.1μgmL-1, ORAC 3355±164 and 2714±79μmoltroloxeq/g, and FRAP 888±16 and 921±9μmoltroloxeq/g) and inhibitory activities against α-amylase (IC50 4.5±0.8 and 1.7±0.3μgmL-1), α-glucosidase (IC50 554.5±158.6 and 787.8±140.6μgmL-1) and glycation (IC50 14.3±3.3 and 16.0±4.2μgmL-1), and lower cytotoxicity, compared to the other fractions and crude ethanol extract. The HPLC-ESI-MS/MS analysis identified various biomolecules known as potent antioxidants, such as chlorogenic acid, (epi)catechin, procyanidins, caffeoyl-hexosides, quercetin-glucosides and kaempferol. The fruit peel of A. crassiflora, a specie from Cerrado, the Brazilian Savanna, provided a source of antioxidant compounds with properties to block carbohydrate digestive enzymes and formation of glycation products. Thus, there is potential to use the by-products of araticum in order to identify and isolate phytochemicals for application in nutraceutical supplements, food additives and pharmaceuticals products.
- Published
- 2016
39. The Role of Metformin in Controlling Oxidative Stress in Muscle of Diabetic Rats
- Author
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Leonardo Gomes Peixoto, Foued Salmen Espindola, Helen Lara Machado, Mariana Nunes Pereira, Renata Roland Teixeira, Danielle Diniz Vilela, Douglas Carvalho Caixeta, Nathalia Belele Baptista, Robinson Sabino-Silva, and Adriele Vieira de Souza
- Subjects
0301 basic medicine ,Male ,Aging ,endocrine system diseases ,medicine.medical_treatment ,medicine.disease_cause ,Biochemistry ,Antioxidants ,Insulin ,chemistry.chemical_classification ,biology ,lcsh:Cytology ,General Medicine ,Catalase ,Metformin ,medicine.anatomical_structure ,Drug Therapy, Combination ,medicine.drug ,Research Article ,medicine.medical_specialty ,Article Subject ,Streptozocin ,Diabetes Mellitus, Experimental ,Superoxide dismutase ,03 medical and health sciences ,Insulin resistance ,Internal medicine ,medicine ,Animals ,Hypoglycemic Agents ,lcsh:QH573-671 ,Rats, Wistar ,Muscle, Skeletal ,Reactive oxygen species ,Electron Transport Complex I ,business.industry ,Superoxide Dismutase ,Skeletal muscle ,Cell Biology ,Streptozotocin ,medicine.disease ,Oxidative Stress ,030104 developmental biology ,Endocrinology ,chemistry ,biology.protein ,Lipid Peroxidation ,business ,Oxidative stress - Abstract
Metformin can act in muscle, inhibiting the complex I of the electron transport chain and decreasing mitochondrial reactive oxygen species. Our hypothesis is that the inhibition of complex I can minimize damage oxidative in muscles of hypoinsulinemic rats. The present study investigated the effects of insulin and/or metformin treatment on oxidative stress levels in the gastrocnemius muscle of diabetic rats. Rats were rendered diabetic (D) with an injection of streptozotocin and were submitted to treatment with insulin (D+I), metformin (D+M), or insulin plus metformin (D+I+M) for 7 days. The body weight, glycemic control, and insulin resistance were evaluated. Then, oxidative stress levels, glutathione antioxidant defense system, and antioxidant status were analyzed in the gastrocnemius muscle of hypoinsulinemic rats. The body weight decreased in D+M compared to ND rats. D+I and D+I+M rats decreased the glycemia and D+I+M rats increased the insulin sensitivity compared to D rats. D+I+M reduced the oxidative stress levels and the activity of catalase and superoxide dismutase in skeletal muscle when compared to D+I rats. In conclusion, our results reveal that dual therapy with metformin and insulin promotes more benefits to oxidative stress control in muscle of hypoinsulinemic rats than insulinotherapy alone.
- Published
- 2016
40. The effect of different training programs on antioxidant status, oxidative stress, and metabolic control in type 2 diabetes
- Author
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Artur Bessa, Renato Oliveira, Guilherme Gularte de Agostini, Paulo Tannús Jorge, Marco Túlio de Mello, Vanessa Neves de Oliveira, Maria Luiza Mendonça Pereira Jorge, and Foued Salmen Espindola
- Subjects
Adult ,Blood Glucose ,Male ,medicine.medical_specialty ,Physiology ,Strength training ,Endocrinology, Diabetes and Metabolism ,Type 2 diabetes ,Kidney ,medicine.disease_cause ,Antioxidants ,Oxygen Consumption ,Physiology (medical) ,Diabetes mellitus ,Internal medicine ,medicine ,Humans ,Aerobic exercise ,Muscle, Skeletal ,Exercise ,Nitrites ,Aged ,Nutrition and Dietetics ,Superoxide Dismutase ,business.industry ,Kidney metabolism ,General Medicine ,Middle Aged ,Catalase ,medicine.disease ,Oxidative Stress ,Endocrinology ,Diabetes Mellitus, Type 2 ,Liver ,Metabolic control analysis ,Body Composition ,Female ,Lipid Peroxidation ,Liver function ,Reactive Oxygen Species ,business ,Biomarkers ,Oxidative stress - Abstract
We compared the effects of 12 weeks of 3 different exercise types on type 2 diabetic (T2DM) male and female human subjects, randomly divided into 4 groups: aerobic training (AT; n = 11), strength training (ST; n = 10), combined training (CBT; n = 10), and no training (NT; n = 12). Metabolic control, anthropometric parameters, lipid and hematologi- cal profiles, kidney and liver function markers, hormones, antioxidant enzymes, and oxidative stress markers were assessed prior to and after the training programs. At baseline, fasting blood glucose and hemoglobin A1c in the ST group were higher than in the NT group; after the training, we no longer observed differences in these groups, suggesting an improvement on these parameters. In the AT group, catalase and superoxide dismutase activity, nitrite concentration, levels of sulfhydryl groups, and peak rate of oxygen consumption were elevated after the training (p < 0.05). No changes were observed in anti- oxidant enzymes or oxidative stress markers in the ST group. The levels of sulfhydryl groups diminished in the NT group (p < 0.01) and increased in the CBT group (p < 0.05). These data demonstrate that the AT program for the T2DM subjects provided important upregulation in antioxidant enzymes and increased nitric oxide bioavailability, which may help minimize oxidative stress and the development of the chronic complications of diabetes. We propose that the beneficial effects ob- served in the metabolic parameters of the ST group occurred in response to the poor baseline metabolic health n this group, and not necessarily in response to the training itself.
- Published
- 2012
41. Alterations of antioxidant biomarkers and type I collagen deposition in the parotid gland of streptozotocin-induced diabetic rats
- Author
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Luciana Karen Calábria, Simone Ramos Deconte, Renato Oliveira, Foued Salmen Espindola, Neire Moura de Gouveia, Alberto S. Moraes, and Vanessa Neves de Oliveira
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Blood Glucose ,Male ,Antioxidant ,medicine.medical_treatment ,medicine.disease_cause ,Antioxidants ,chemistry.chemical_compound ,Malondialdehyde ,Parotid Gland ,Urea ,Acarbose ,Chemistry ,Alanine Transaminase ,Blood Proteins ,Free Radical Scavengers ,gamma-Glutamyltransferase ,General Medicine ,Parotid gland ,Cholesterol ,medicine.anatomical_structure ,Creatinine ,Type I collagen ,medicine.drug ,medicine.medical_specialty ,Collagen Type I ,Streptozocin ,Diabetes Mellitus, Experimental ,Diabetes mellitus ,Internal medicine ,medicine ,Animals ,Hypoglycemic Agents ,Aspartate Aminotransferases ,Rats, Wistar ,General Dentistry ,Triglycerides ,Glutathione Peroxidase ,Superoxide Dismutase ,Fructose ,Cell Biology ,Alkaline Phosphatase ,medicine.disease ,Streptozotocin ,Rats ,Oxidative Stress ,Endocrinology ,Otorhinolaryngology ,Biomarkers ,Oxidative stress - Abstract
Background and objective Acarbose is a competitive inhibitor of intestinal alpha-glycosidases that slows the breakdown of sucrose and starch, thereby reducing glucose and fructose absorption. The aim of this study was to evaluate the effect of acarbose treatment on antioxidant parameters and deposition of type I collagen in the parotid glands of diabetic rats. Methods Diabetes mellitus was induced by intravenous injection of streptozotocin, and rats were divided into four groups: non-diabetic (NDM), diabetic (DM), diabetic treated with 25 mg/kg acarbose (DMA) and non-diabetic treated with acarbose (NDMA). Changes in enzymatic antioxidant systems, such as the activity of SOD and GPx enzymes, were evaluated, and the specific staining pattern of the type I collagen fibres was investigated in the rat parotid glands. Results The DM group presented high levels of SOD and GPx enzymes, which were reduced by acarbose treatment. Tissue damage, which was indicated by an increased MDA concentration in the parotid glands of rats in the DM group, was also reversed in the DMA group. Moreover, type I collagen fibres from DM rats were more intensely stained than those of NDM rats. Acarbose treatment was effective in decreasing collagen deposition, which was shown by a decrease in staining intensity of approximately 25%. Conclusions These results suggest that the diabetic state influences the type I collagen concentration in the parotid glands of rats. In addition, acarbose treatment was helpful in preventing the deposition of such fibres, as well the increase in oxidative stress induced by hyperglycemia.
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- 2011
42. Adaptogenic potential of royal jelly in liver of rats exposed to chronic stress
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Foued Salmen Espindola, Nathalia Belele Baptista, Renata Roland Teixeira, Leonardo Gomes Peixoto, Douglas Carvalho Caixeta, Helen Lara Machado, Celso Rodrigues Franci, Danielle Diniz Vilela, and Adriele Vieira de Souza
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Blood Glucose ,Male ,0301 basic medicine ,Antioxidant ,Physiology ,medicine.medical_treatment ,lcsh:Medicine ,Social Sciences ,medicine.disease_cause ,Biochemistry ,Antioxidants ,chemistry.chemical_compound ,0302 clinical medicine ,Corticosterone ,Royal jelly ,Medicine and Health Sciences ,Psychology ,Chronic stress ,HOMEOSTASE ,lcsh:Science ,Multidisciplinary ,biology ,Physics ,Fatty Acids ,Classical Mechanics ,Glutathione ,Blood Sugar ,Enzymes ,Body Fluids ,Cold Temperature ,Dismutases ,Blood ,Liver ,Physiological Parameters ,Physical Sciences ,Mechanical Stress ,Anatomy ,Research Article ,medicine.medical_specialty ,food.ingredient ,Psychological Stress ,Blood sugar ,Superoxide dismutase ,Immobilization ,03 medical and health sciences ,food ,Stress, Physiological ,Internal medicine ,Mental Health and Psychiatry ,medicine ,Animals ,Rats, Wistar ,Superoxide Dismutase ,business.industry ,lcsh:R ,Body Weight ,Biology and Life Sciences ,Proteins ,Cell Biology ,Rats ,Oxidative Stress ,Thermal Stresses ,030104 developmental biology ,Endocrinology ,chemistry ,Chronic Disease ,Enzymology ,biology.protein ,lcsh:Q ,Peptides ,business ,030217 neurology & neurosurgery ,Oxidative stress - Abstract
Restraint and cold stress increase both corticosterone and glycemia, which lead to oxidative damages in hepatic tissue. This study assessed the effect of royal jelly (RJ) supplementation on the corticosterone level, glycemia, plasma enzymes and hepatic antioxidant system in restraint and cold stressed rats. Wistar rats were allocated into no-stress, stress, no-stress supplemented with RJ and stress supplemented with RJ groups. Initially, RJ (200mg/Kg) was administered for fourteen days and stressed groups were submitted to chronic stress from the seventh day. The results showed that RJ supplementation decreases corticosterone levels and improves glycemia control after stress induction. RJ supplementation also decreased the body weight, AST, ALP and GGT. Moreover, RJ improved total antioxidant capacity, SOD activity and reduced GSH, GR and lipoperoxidation in the liver. Thus, RJ supplementation reestablished the corticosterone levels and the hepatic antioxidant system in stressed rats, indicating an adaptogenic and hepatoprotective potential of RJ.
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- 2018
43. Phaseolamin treatment prevents oxidative stress and collagen deposition in the hearts of streptozotocin-induced diabetic rats
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Vanessa Neves de Oliveira, Luciana Karen Calábria, Foued Salmen Espindola, Renato Oliveira, Alberto S. Moraes, and Simone Ramos Deconte
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Blood Glucose ,Male ,medicine.medical_specialty ,Antioxidant ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,medicine.disease_cause ,Antioxidants ,Streptozocin ,Diabetes Mellitus, Experimental ,Lipid peroxidation ,chemistry.chemical_compound ,Diabetes mellitus ,Internal medicine ,Internal Medicine ,medicine ,Animals ,Rats, Wistar ,chemistry.chemical_classification ,Reactive oxygen species ,biology ,business.industry ,Heart ,medicine.disease ,Streptozotocin ,Rats ,Disease Models, Animal ,Oxidative Stress ,Endocrinology ,chemistry ,Catalase ,biology.protein ,Phaseolamin ,Collagen ,Plant Lectins ,Cardiology and Cardiovascular Medicine ,business ,Oxidative stress ,medicine.drug - Abstract
The development of cardiovascular complications in patients with diabetes is often associated with an imbalance between reactive oxygen species and antioxidant systems. This imbalance can contribute to high cardiac collagen content, which increases cross-linking and the stiffness of the myocardium. In this study, the protective effect of phaseolamin against damage under oxidative stress and collagen deposition in the cardiac tissue in association with diabetes was evaluated. Non-diabetic and diabetic animals were distributed into groups and treated for 20 days with commercial phaseolamin. The phaseolamin treatment increased total antioxidant activity but reduced the following in diabetic rats: (a) hyperglycaemic state, (b) catalase and superoxide dismutase activity and (c) tissue damage caused by lipid peroxidation. Additionally, the phaseolamin treatment attenuated the collagen levels compared to non-treated diabetic rats. Thus, the short-term anti-hyperglycaemic effect of the phaseolamin treatment may prevent the initial changes caused by oxidative stress and the deposition of collagen, as well as reduce the incidence of heart complications.
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- 2014
44. Adaptogenic potential of royal jelly in liver of rats exposed to chronic stress.
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Caixeta, Douglas Carvalho, Teixeira, Renata Roland, Peixoto, Leonardo Gomes, Machado, Helen Lara, Baptista, Nathalia Belele, de Souza, Adriele Vieira, Vilela, Danielle Diniz, Franci, Celso Rodrigues, and Salmen Espindola, Foued
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CORTICOSTERONE ,PHYSIOLOGICAL effects of cold temperatures ,ROYAL jelly ,LABORATORY rats ,ANTIOXIDANTS ,THERAPEUTICS - Abstract
Restraint and cold stress increase both corticosterone and glycemia, which lead to oxidative damages in hepatic tissue. This study assessed the effect of royal jelly (RJ) supplementation on the corticosterone level, glycemia, plasma enzymes and hepatic antioxidant system in restraint and cold stressed rats. Wistar rats were allocated into no-stress, stress, no-stress supplemented with RJ and stress supplemented with RJ groups. Initially, RJ (200mg/Kg) was administered for fourteen days and stressed groups were submitted to chronic stress from the seventh day. The results showed that RJ supplementation decreases corticosterone levels and improves glycemia control after stress induction. RJ supplementation also decreased the body weight, AST, ALP and GGT. Moreover, RJ improved total antioxidant capacity, SOD activity and reduced GSH, GR and lipoperoxidation in the liver. Thus, RJ supplementation reestablished the corticosterone levels and the hepatic antioxidant system in stressed rats, indicating an adaptogenic and hepatoprotective potential of RJ. [ABSTRACT FROM AUTHOR]
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- 2018
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45. Interdisciplinary therapy changes superoxide dismutase activity and adiponectin in obese adolescents: a randomised controlled trial.
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Nunes, João Elias Dias, Cunha, Heitor Santos, Freitas, Zulmária Rezende, Nogueira, Ana Maria Caixeta, Dâmaso, Ana Raimunda, Espindola, Foued Salmen, and Cheik, Nadia Carla
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TREATMENT of childhood obesity ,ANTIOXIDANTS ,C-reactive protein ,CARDIOPULMONARY system ,STATISTICAL correlation ,EXERCISE ,EXERCISE tests ,GROUP psychotherapy ,HEALTH care teams ,INFLAMMATION ,NUTRITION education ,PROBABILITY theory ,RESEARCH funding ,STATISTICS ,SUPEROXIDE dismutase ,T-test (Statistics) ,DATA analysis ,OXIDATIVE stress ,TREADMILLS ,BODY mass index ,RANDOMIZED controlled trials ,TREATMENT effectiveness ,OXYGEN consumption ,DATA analysis software ,ADIPONECTIN ,MANN Whitney U Test - Abstract
The objective of this study is to evaluate the effect of interdisciplinary therapy in the parameters of the oxidative stress and the anti-inflammatory responses of obese adolescents. We selected 57 participants, who were randomly divided into 2 groups: interdisciplinary therapy group and a control group. After 6 months of intervention, 17 participants of the interdisciplinary therapy group and 8 of the control group returned for re-evaluation. The interdisciplinary therapy group participated in a treatment with 4 weekly sessions of exercise, a weekly group therapy session and a weekly nutritional education session. Blood parameters of oxidative stress and anti-inflammatory response were evaluated. The results demonstrated that there were significant increases in the interdisciplinary therapy group for superoxide dismutase activity (6.56 ± 3.22 to 11.40 ± 7.49) and ferric-reducing antioxidant potential concentration (532.91 ± 106.48 to 573.25 ± 112.57), although adiponectin levels did not reduce (40.9 ± 29.34 to 49.05 ± 41.22). A significant decrease in nitrite levels was also found (14.23 ± 8.48 to 11.45 ± 6.05). In the control group, significant reduction was found in adiponectin (31.56 ± 18.88 to 18.01 ± 11.66). This study suggests that interdisciplinary therapy for 6 months was effective in improving the anti-inflammatory responses and the antioxidant defences in obese adolescents. [ABSTRACT FROM PUBLISHER]
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- 2016
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46. Antioxidants in Hops: Bioavailability, Health Effects and Perspectives for New Products.
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Zugravu, Corina-Aurelia, Bohiltea, Roxana-Elena, Salmen, Teodor, Pogurschi, Elena, and Otelea, Marina Ruxandra
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BIOAVAILABILITY ,NEW product development ,DIETARY supplements ,METABOLIC syndrome ,ANTIOXIDANTS ,SECONDARY prevention ,HOPS - Abstract
Hop plant (Humulus lupulus L.) has been used by humans for ages, presumably first as a herbal remedy, then in the manufacturing of different products, from which beer is the most largely consumed. Female hops cones have different useful chemical compounds, an important class being antioxidants, mainly polyphenols. This narrative review describes the main antioxidants in hops, their bioavailability and biological effects, and the results obtained by now in the primary and secondary prevention of several non-communicable diseases, such as the metabolic syndrome related diseases and oncology. This article presents in vitro and in vivo data in order to better understand what was accomplished in terms of knowledge and practice, and what needs to be clarified by additional studies, mainly regarding xantohumol and its derivates, as well as regarding the bitter acids of hops. The multiple protective effects found by different studies are hindered up to now by the low bioavailability of some of the main antioxidants in hops. However, there are new promising products with important health effects and perspectives of use as food supplements, in a market where consumers increasingly search for products originating directly from plants. [ABSTRACT FROM AUTHOR]
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- 2022
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47. The effect of different training programs on antioxidant status, oxidative stress, and metabolic control in type 2 diabetes.
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Oliveira, Vanessa Neves de, Bessa, Artur, Jorge, Maria Luiza Mendonça Pereira, Oliveira, Renato José da Silva, de Mello, Marco Túlio, De Agostini, Guilherme Gularte, Jorge, Paulo Tannus, and Espindola, Foued Salmen
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TYPE 2 diabetes treatment ,SALIVA analysis ,ANALYSIS of variance ,ANTIOXIDANTS ,BLOOD testing ,BLOOD pressure ,BLOOD sugar ,FISHER exact test ,MATHEMATICAL statistics ,LIPID peroxidation (Biology) ,NITRIC oxide ,TYPE 2 diabetes ,NONPARAMETRIC statistics ,HEALTH outcome assessment ,RESEARCH funding ,STATISTICAL sampling ,STATISTICS ,DATA analysis ,PARAMETERS (Statistics) ,OXIDATIVE stress ,PHYSICAL training & conditioning ,TREATMENT effectiveness ,DESCRIPTIVE statistics - Abstract
Copyright of Applied Physiology, Nutrition & Metabolism is the property of Canadian Science Publishing and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 2012
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48. Topic use of Annona crassiflora Mart. contributes to wound healing due to the antioxidant and proliferative effects of fibroblasts.
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de Moura, Francyelle Borges Rosa, Ferreira, Bruno Antonio, Muniz, Elusca Helena, Justino, Allisson Benatti, Silva, Ana Gabriela, Santos, Rinara de Almeida, Gomide, José Augusto Leoncio, Ribeiro, Rosy Iara Maciel de Azambuja, Ribeiro, Daniele Lisboa, Araújo, Fernanda de Assis, Espindola, Foued Salmen, and Tomiosso, Tatiana Carla
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WOUND healing , *ANNONA , *FIBROBLASTS , *FRUIT skins , *PHENOLS , *GRAPEFRUIT , *ORANGES , *ANTIOXIDANTS , *PLANTS , *PLANT extracts , *ANIMALS , *MICE - Abstract
Annona crassiflora Mart. is a species native to the Cerrado biome, whose fruit is known as araticum or marolo. Plant parts are widely used in folk medicine to treat inflammation and pain associated with rheumatism, wounds, venereal diseases, snakebites, and microbial infections. Thus, we investigated a fraction rich in phenolic compounds (PCAc) obtained from the crude extract of the peel of these fruits on non-cytotoxic, anti-inflammatory, antioxidant, and collagen biosynthesis properties in the healing of wounds induced on the back of BALB/c mice. For the control group, the induced wounds were not treated and for the others, wounds were treated topically with vehicle or vehicle plus PCAc. Both fractions contained in PCAc demonstrated effective protection on fibroblasts. We highlight the effect of the ethyl acetate fraction which, in addition to the protective effect, has a proliferative activity on these cells. In addition, PCAc caused improvement in healing after 7 days of treatment and in the longest period of treatment with PCAc (7, 14, and 21 days) there was a greater contraction of the wound, accompanied by resolution of the inflammatory process, antioxidant defense, increasing collagen synthesis, and modulation of metalloproteinases. PCAc demonstrated better re-epithelialization and organization of the dermis at the end of treatment. The changes promoted by the phenolic compounds of A. crassiflora were important in the healing process, especially in activities related to inflammation, oxidative stress, and fibrogenesis. [ABSTRACT FROM AUTHOR]
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- 2022
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49. Bioactive compounds from the leaves of Maytenus ilicifolia Mart. ex Reissek: Inhibition of LDL oxidation, glycation, lipid peroxidation, target enzymes, and microbial growth.
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Bittar, Vinicius Prado, Silva Borges, Ana Luiza, Justino, Allisson Benatti, Carrillo, Maria Sol Peña, Mateus Duarte, Rener Francisco, Silva, Nagela Bernadelli Sousa, Gonçalves, Daniela Silva, Prado, Diego Godina, Araújo, Iasmin Aparecida Cunha, Martins, Mário Machado, Gomes Martins, Carlos Henrique, Botelho, Françoise Vasconcelos, Silva, Neide Maria, de Oliveira, Alberto, and Espíndola, Foued Salmen
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EXPERIMENTAL design , *HIGH performance liquid chromatography , *TERPENES , *FLAVONOIDS , *ANIMAL experimentation , *ALKALOIDS , *GASTRITIS , *LOW density lipoproteins , *ANTIOXIDANTS , *ORGANIC compounds , *TANNINS , *PHYTOCHEMICALS , *RATS , *PLANT extracts , *ETHANOL , *CELL lines , *OXIDATION-reduction reaction , *LIPID peroxidation (Biology) , *ANIMALS - Abstract
Maytenus ilicifolia Mart. ex Reissek, a medicinal plant used for treating gastritis, ulcers, and gastric disorders, possesses therapeutic properties attributed to diverse leaf compounds—terpenoids, alkaloids, flavonoids, phenols, and tannins, reflecting the ethnopharmacological knowledge of traditional users. We aimed to assess the antioxidant and antiglycant capacities of Maytenus ilicifolia's ethanolic extract and organic fractions, identify bioactive compounds through HPLC-MS/MS analysis, and conduct phytochemical assessments. We also assessed their potential to inhibit digestive and cholinesterase enzymes, mitigate oxidation of human LDL and rat hepatic tissue, and examine their antimicrobial and cytotoxic properties. Organic fractions (hexane - HF-Mi, dichloromethane - DMF-Mi, ethyl acetate - EAF-Mi, n-butanol - BF-Mi, and hydromethanolic - HMF-Mi) were obtained via liquid-liquid partitioning. Antioxidant (DPPH, FRAP, ORAC) and antiglycant (BSA/FRU, BSA/MGO, ARG/MGO/LDL/MGO models) capacities were tested. Phytochemical analysis employed HPLC-MS/MS. We also studied the inhibitory effects on α-amylase, acetylcholinesterase, butyrylcholinesterase, human LDL and rat hepatic tissue oxidation, antimicrobial activity, and cytotoxicity against RAW 264.7 macrophages. HPLC-ESI-MS/MS identified antioxidant compounds such as catechin, quercetin, and kaempferol derivatives. Ethanolic extract (EE-Mi) and organic fractions demonstrated robust antioxidant and antiglycant activity. EAF-Mi and BF-Mi inhibited α-amylase (2.42 μg/mL and 7.95 μg/mL) compared to acarbose (0.144 μg/mL). Most organic fractions exhibited ∼50% inhibition of acetylcholinesterase and butyrylcholinesterase, rivaling galantamine and rivastigmine. EAF-Mi, BF-Mi, and EE-Mi excelled in inhibiting lipid peroxidation. All fractions, except HMF-Mi, effectively countered LDL oxidation, evidenced by the area under the curve. These fractions protected LDL against lipid peroxidation. This study unveils Maytenus ilicifolia's ethanolic extract and organic fractions properties. Through rigorous analysis, we identify bioactive compounds and highlight their antioxidant, antiglycant, enzyme inhibition, and protective properties against oxidative damage. These findings underline its significance in modern pharmacology and its potential applications in healthcare. [Display omitted] • Quercetin, kaempferol and catechin derivatives were identified using HPLC-ESI-MS/MS. • M. ilicifolia inhibits liver tissue peroxidation in the Fe2+-ascorbate model. • M. ilicifolia inhibits human LDL oxidation, peroxidation and glycation in vitro. • Ethyl acetate fraction exhibited notable antiglycant activity in BSA/FRUTOSE model. [ABSTRACT FROM AUTHOR]
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- 2024
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50. Antioxidant and anti-glycation capacities of some medicinal plants and their potential inhibitory against digestive enzymes related to type 2 diabetes mellitus.
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Franco, Rodrigo Rodrigues, da Silva Carvalho, Danúbia, de Moura, Francyelle Borges Rosa, Justino, Allisson Benatti, Silva, Heitor Cappato Guerra, Peixoto, Leonardo Gomes, and Espindola, Foued Salmen
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TYPE 2 diabetes treatment , *AMYLASES , *ANTIOXIDANTS , *ANALYTICAL chemistry , *DIGESTION , *ENZYMES , *ETHANOL , *GLYCOSYLATION , *LIPASES , *MEDICINAL plants , *DESCRIPTIVE statistics - Abstract
Ethnopharmacological relevance Plants preparations are used by traditional medicine in the treatment of various diseases, such as type-2 diabetes mellitus. Some medicinal plants are capable of controlling the complications of this metabolic disease at different levels, for example, providing antioxidant compounds that act against oxidative stress and protein glycation and others which are capable of inhibiting the catalysis of digestive enzymes and thus contribute to the reduction of hyperglycemia and hyperlipidemia. Our objective was to investigate the antioxidant and anti-glycation activities of some medicinal plants and their potential inhibitory against α-amylase, α-glucosidase and pancreatic lipase activities. Material and methods Based on the ethnobotanical researches carried out by academic studies conducted at the Federal University of Uberlandia, ten plants traditionally used in the treatment of type-2 diabetes mellitus were selected. Ethanol (EtOH) and hexane (Hex) extracts of specific parts of these plants were used in enzymatic assays to evaluate their inhibitory potential against α-amylase, α-glucosidase and lipase, as well as their antioxidant (DPPH, ORAC and FRAP) and anti-glycation (BSA/fructose model) capacities. Results The results indicate that EtOH extract of four of the ten analyzed plants exhibited more than 70% of antioxidant and anti-glycation capacities, and α-amylase and lipase inhibitory activities; no extract was able to inhibit more than 40% the α-glucosidase activity. The EtOH extracts of Bauhinia forficata and Syzygium. cumini inhibited α-amylase (IC 50 8.17 ± 2.24 and 401.8 ± 14.7 μg/mL, respectively), whereas EtOH extracts of B. forficata, Chamomilla recutita and Echinodorus grandiflorus inhibited lipase (IC 50 59.6 ± 10.8, 264.2 ± 87.2 and 115.8 ± 57.1 μg/mL, respectively). In addition, EtOH extracts of B. forficata, S. cumini, C. recutita and E. grandiflorus showed, respectively, higher antioxidant capacity (DPPH IC 50 0.7 ± 0.1, 2.5 ± 0.2, 1.3 ± 0.2 and 35.3 ± 9.0 μg/mL) and anti-glycation activity (IC 50 22.7 ± 4.4, 246.2 ± 81.7, 18.5 ± 2.8 and 339.0 ± 91.0 μg/mL). Conclusions EtOH extracts of four of the ten species popularly cited for treatment of type 2 diabetes mellitus have shown promising antioxidant and anti-glycation properties, as well as the ability to inhibit the digestive enzymes α-amylase and lipase. Thus, our results open new possibilities for further studies in order to evaluate the antidiabetic potential of these medicinal plants. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
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