1. Efficient extraction, antioxidant activities and anti-inflammation of polysaccharides from Notopterygium franchetii Boiss.
- Author
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Wang W and Liu J
- Subjects
- Animals, Embryo, Nonmammalian drug effects, Embryo, Nonmammalian embryology, Free Radical Scavengers pharmacology, Larva drug effects, Oxidation-Reduction drug effects, Plant Extracts isolation & purification, Plant Roots chemistry, Polysaccharides isolation & purification, Zebrafish embryology, Anti-Inflammatory Agents pharmacology, Antioxidants pharmacology, Apiaceae chemistry, Plant Extracts pharmacology, Polysaccharides pharmacology
- Abstract
To optimize the extraction of polysaccharides from Notopterygium franchetii Boiss (NFP), microwave-assisted aqueous two phase system (MAATPS) and response surface methodology (RSM) were employed. Results showed that the optimum conditions of MAATPS were as follows: ethanol concentration of 32 % (w/w), (NH
4 )2 SO4 concentration of 24 % (w/w) and extraction time of 15 min. Under such condition, YS (the recovery of polysaccharides) was 80.57 % with 90.40 % of YP (the recovery of proteins). In addition, analysis of the physicochemical properties of extracted polysaccharides (NFP-MAATPS) under the optimal conditions indicated that compared to polysaccharides extracted by traditional extraction method (NFP-HWE), NFP-MAATPS exhibited higher extraction efficiency, decreased average molecular weight, different monosaccharide compositions and mole ratios. Moreover, NFP-MAATPS exhibited stronger antioxidant activity in vitro and more potent anti-inflammatory activity in zebrafish embryos than NFP-HWE. Our results suggest that purified NFP-MAATPS could be potentially used in complementary medicines or functional foods., (Copyright © 2020 Elsevier Ltd. All rights reserved.)- Published
- 2020
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