Search

The use of an electrochemical approach for assessment of the oxidative stress severity is a promising direction for point-of-care testing development, which is especially important for critically ill patients. The aim of this study was to determine the influence of different types of blood collection tubes (with clot activator and separating gel, lithium heparin, sodium citrate and K2EDTA) and storage conditions (at 25, 4 and –23 °C up to 5 days) on the electrochemical analysis of the antioxidant status of blood plasma, assessed by measuring the open circuit potential of platinum electrode (OCP) and antioxidant capacity via cyclic voltammetry method (q). It was obtained that blood collection tubes with lithium heparin and clot activator are the most suitable for the electrochemical analysis of antioxidant status of blood plasma, since they do not affect the results of measurements. Furthermore, data obtained during storage blood plasma samples in different temperature conditions indicate that it is preferable to perform electrochemical analysis in fresh samples.

In this work we used electrochemical methods, open circuit potential (OCP) measurement with platinum electrode, and cyclic voltammetry (CV) in blood serum to assess redox states in patients with polytrauma (n=56) that allow to evaluate probability of survival early on, and therefore have the potential to improve therapy outcomes. Electrochemical assessment of redox states in sera samples correlated with traditional methods of prooxidant/antioxidant balance quantification. OCP and quantity of electricity passed (Q), calculated from CVs, were significantly different between the survivors and non‐survivors. Patients with Q>22 μC (day 1) and OCP>34 mV (day 3) had low chance of survival. [ABSTRACT FROM AUTHOR]