1. Phyto-assisted synthesis of bio-functionalised silver nanoparticles and their potential anti-oxidant, anti-microbial and wound healing activities.
- Author
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Mohanta YK, Biswas K, Panda SK, Bandyopadhyay J, De D, Jayabalan R, Bastia AK, and Mohanta TK
- Subjects
- Cell Line, Gram-Negative Bacteria drug effects, Gram-Positive Bacteria drug effects, Humans, Microbial Sensitivity Tests, Spectrophotometry, Ultraviolet, Spectroscopy, Fourier Transform Infrared, Anti-Infective Agents pharmacology, Antioxidants pharmacology, Ardisia chemistry, Metal Nanoparticles chemistry, Plant Extracts pharmacology, Silver chemistry, Wound Healing drug effects
- Abstract
Bio- synthesis of silver nanoparticles (AgNPs) was made by using the aqueous leaf extract of Ardisia solanacea. Rapid formation of AgNPs was observed from silver nitrate upon treatment with the aqueous extract of A. solanacea leaf. The formation and stability of the AgNPs in the colloidal solution were monitored by UV-visible spectrophotometer. The mean particle diameter of AgNPs was calculated from the DLS with an average size ∼4 nm and ∼65 nm. ATR-FTIR spectroscopy confirmed the presence of alcohols, aldehydes, flavonoids, phenols and nitro compounds in the leaf which act as the stabilizing agent. Antimicrobial activity of the synthesized AgNPs was performed using agar well diffusion and broth dilution method against the Gram-positive and Gram-negative bacteria. Further, robust anti-oxidative potential was evaluated by DPPH assay. The highest antimicrobial activity of synthesized AgNPs was found against Pseudomonas aeruginosa (28.2 ± 0.52 mm) whereas moderate activity was found against Bacillus subtilis (16.1 ± 0.76), Candida kruseii (13.0 ± 1.0), and Trichophyton mentagrophytes (12.6 ± 1.52). Moreover, the potential wound healing activity was observed against the BJ-5Ta normal fibroblast cell line. Current research revealed that A. solanacea was found to be a suitable source for the green synthesis of silver nanoparticles.
- Published
- 2017
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