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Introduction: Phytochemicals are used in many products, including foods, beverages, pharmaceuticals, and cosmetics. The extraction of phytochemicals is considered one of the best solutions to valorize these underestimated materials. Many methods have been developed to efficiently extract phytochemicals at high quality, high purity, and low costs without harming the environment. Recently, molecular hydrogen (H 2 ) has shown its ability to improve the extraction of phytochemicals from plant materials. Due to its unique physicochemical and biological properties, H 2 showed an efficient ability to extract phenolics and antioxidants at high yields with cost-effective potential. Without sophisticated equipment and high energy and solvent consumption, the hydrogen extraction method is a green and applicable alternative for the extraction of phytochemicals., Objectives: This review aims to provide the latest knowledge and results concerning the studies on using hydrogen-rich solvents to extract phytochemicals from different agri-food wastes, by-products, and other plant materials., Materials and Methods: Recent literature relating to extracting phytochemicals by the hydrogen-rich solvent method and its potential mechanisms is summarized to provide a basic understanding of how hydrogen can improve the extraction of phytochemicals., Results: This review describes, for the first time, the practical procedure of how researchers and laboratories can apply the hydrogen extraction method under safe conditions at a low-budget scale. The review provides some examples of the hydrogen extraction method and the mechanisms and rationale behind its effectiveness., Conclusions: It can be concluded that the hydrogen-rich solvent method is a green and cost-effective method for extracting phytochemicals from different plant materials., (© 2023 The Author. Phytochemical Analysis published by John Wiley & Sons Ltd.)

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Different methods are used for extracting phytochemicals from plant produce. Some ‎methods require sophisticated and expensive instruments, while others need multiple steps ‎and specialist equipment, leading to an increase in energy, solvents, cost, and time. Here, the effect of incorporating hydrogen (H 2) into solvents (that is water, ethanol, ‎methanol) on the extraction of flavonoids (TFC), phenolics (TPC), anthocyanins ‎‎(TAC), and antioxidants (DPPH and ABTS), as well as the extraction yield from red ‎beetroot, were evaluated. The biggest extraction yield was with the use of H 2 -rich ‎methanol (HRM) (24.32%). Incorporating H 2 into water, ethanol, and methanol ‎led to a significant increase in TPC by 77.34%, 39.02% and 89.07%, in the TFC by ‎‎43.30%, 50.5% and 88.87%, in the TAC by 92.62%, 199.5% and 257.41%. DPPH ‎scavenging activity increased by 6.49%, 7.05% and 12.15%, and ABTS scavenging ‎activity by 3.93%, 9.82% and 15.05%, respectively. The extraction of individual ‎phenolic compounds, using various solvents, were different for each compound ‎investigated, with HPLC analysis showing that the levels of some phenolics increased ‎when H 2 was incorporated into the solvents. ‎This method, i.e., addition of H 2 gas, is ‎suggested as a relatively cheap and practical method for increasing the extraction of ‎phytochemicals using an eco-friendly method. [Display omitted] • The highest extraction yield was found for hydrogen-rich methanol. • H 2 incorporation ‎increased phenolics, flavonoids, anthocyanins, and antioxidants. • ‎The best extraction of all phytochemicals was HRM. • H 2 incorporation is an inexpensive,‎ eco-friendly, and green method. [ABSTRACT FROM AUTHOR]

Antibacterial and antioxidant activities form an essential component of the bioactive properties of plant products. The antibacterial and the antioxidant properties of three types of aqueous extracts of four herbs and spices were evaluated. While the total phenolic content, DPPH radical scavenging activity and oxidoreduction potential methods were used for the antioxidant properties determination, the inhibition diameters of extracts toward the growth of Staphylococcus warneri, Bacillus cereus, Enterobacter cloacae and Proteus vulgaris were evaluated. Although the phenolic content did not correlate with the antibacterial activity of plant extracts, it linearly correlated (R2 = 0.827) with DPPH scavenging activity of the studied plants following the order: sumac > ginger > rosemary > cinnamon. The oxidoreduction potential values linearly correlated with both the phenolic content (R2 ≈ 0.88) and DPPH scavenging activity values (R2 ≈ 0.96). The oxidoreduction potential could be proposed as a useful companion tool combined with other techniques when determining the antioxidant activity of plant extracts and food products is considered. [ABSTRACT FROM AUTHOR]