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Phenolic compounds are the predominant chemical constituents in the secondary metabolites of plants and are commonly found in pears. In this study, we focused on the analysis of the phenolic composition and antioxidant activity of leaves from five pear cultivars (Cuiguan, Chaohong, Kuerle, Nanguoli, and Yali) and tea leaves (Fudingdabai as the control) using ultra-performance liquid chromatography coupled with electrospray ionization triple quadrupole mass spectrometry. The results indicated significant differences in the amount and composition of phenolic metabolites between tea and pear leaves, as well as among the five pear varieties. Only approximately one-third of the metabolites exhibited higher levels in pear leaves compared to that in tea leaves. The total phenol content in the Yali cultivar was higher than that in the other pear cultivars. Furthermore, specific phenolic metabolites with high expression were identified in the leaves of different groups. The levels of delphinidin 3-glucoside, aesculin, prunin, cosmosiin, quercetin 3-galactoside, isorhamnetin-3-O-glucoside, nicotiflorin, narcissin, chlorogenic acid, and cryptochlorogenic acid were relatively high among the five pear cultivars. (-)-Gallocatechin gallate, 6-methylcoumarin, aesculetin, hesperidin, kaempferol, and caftaric acid were identified as specific metabolic substances unique to each type of pear leaf. Most of the differential metabolites showed positive correlations and were primarily enriched in the flavonoid biosynthesis, flavone and flavonol biosynthesis, and phenylpropanoid biosynthesis pathways. DPPH (1,1-Diphenyl-2-picrylhydrazyl radical) analysis indicated that the Yali cultivar exhibited the highest antioxidant activity compared to other varieties. This systematic analysis of the differences in phenolic metabolite composition and antioxidant activity between pear and tea leaves provides a theoretical foundation for the development and utilization of pear leaf resources. [ABSTRACT FROM AUTHOR]

Light-emitting diodes (LEDs) are the promising technology with low heat production and feasible spectra optimization, which improve plants' function. In current study, basil microgreens as novel-leafy vegetables grown under LEDs (blue, red, 30%blue +70%red, and white) and sunlight to investigate phytochemical properties by comparing to sunlight-treated mature basil. Results showed that narrow wavelength including blue, red and blue/red LEDs had significant effect on increasing total phenol content (TPC) and antiradical activity (AA) of microgreens. Blue LED-treated microgreens exhibited significant increase in Potassium and the best visual appearance in comparison with mature and other microgreens. In the case of essential oils, methyl chavicol was the major compound in all samples with the main effect of broad light spectra; red LED- and blue LED-treated microgreens represented the maximum and minimum of methyl eugenol and β-caryophyllene, respectively, which could be related to the significant effect of red light on essential oil biosynthesis. No significant difference was observed in the market-acceptability of mature basil and microgreens which demonstrated that LEDs had no negative impact on organoleptic properties. Throughout the storage, darkness-stored microgreens showed the best result with the least weight loss and decrease in TPC; however, brightness-yellowness variation observed due to the plants' aging. [ABSTRACT FROM AUTHOR]

Dietary supplements (DS) are products that are recommended for the treatment of various health issues, especially during the global pandemic, and the accuracy of the labeling information of these supplements plays a significant role in human health. In this study, a novel high performance liquid chromatography-diode array detector (HPLC-DAD) method was developed and validated to determine the accuracy of the labeling information of commercial dietary supplements containing phenolic compounds (rutin, quercetin, and resveratrol). The mobile phase, flow rate, and column temperature were optimized. Validation studies were carried out to prove the validity of the developed method. The limit of detection (LOD) and limit of quantification (LOQ) values of rutin, quercetin, and resveratrol were found in the range of 4.83-6.62 ng/mL and 16.09-22.07 ng/mL, respectively. Recoveries% were calculated in the range of 99.52-100.19% and 99.78-100.34% in the intra-day and inter-day analysis, respectively. The IC50 values of the dietary supplement extracts obtained by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method ranged from 0.08 to 0.76 mg/mL. Total Antioxidant Capacity (TAC) values of DS1, DS2, DS3, DS4, DS5, measured employing the Cupric Reducing Antioxidant Capacity (CUPRAC) method, were found to be 0.09, 0.03, 0.10, 0.10 and 0.40 mmol trolox per gram of extract, respectively. [ABSTRACT FROM AUTHOR]

The optimal conditions for polyphenol extraction from potato peel using conventional solvent extraction (CSE) and ultrasound-assisted extraction (UAE) methods were predicted to estimate the highest amounts of polyphenols by exploiting a response surface methodology (RSM). The effects of several independent variables, including extraction time, type, and concentration of solvents, on responses (yield, phenolic, flavonoid, and antioxidant activity) were investigated. In the next stage, the potato peel extract (PPE) was incorporated into the low-density polyethylene (LDPE) using plasma treatment to produce an active polymeric film containing natural antioxidants. Polynomial models were significantly fitted to the experimental data based on the Design-Expert software. The highest amounts of responses including yield (9.54%), total phenolic content (TPC) (969.22 mg GAE/100 g dried weight (DW)), total flavonoid content (TFC) (266.53 mg QE/100 g DW), and DPPH (92.36%) in the UAE method, and yield (8.88%), TPC (701.73 mg GAE/100 g DW), TFC (167.4 mg QE/100 g DW), and DPPH (72.28%) in the CSE method were achieved under optimal conditions. Overall, a good agreement between predicted and experimental values was observed under optimal conditions. Besides, the ATR-FTIR results showed the proper attachment of polyphenol compounds on the LDPE using plasma treatment. [ABSTRACT FROM AUTHOR]

Sea buckthorn berries (SBB) are well known for being rich in natural bioactive compounds with high pharmacological activity. In this study, the optimization of extraction parameters was performed to recover phenolic compounds with high antioxidant activity from SBB. This study involved a systematic optimization approach, including screening for a variety of parameters, including temperature, time, ethanol concentration, agitation, and solid loading. On the basis of Plackett–Burman design (PBD) model, the two most significant parameters (agitation and solid loading) were selected, and the correlation model between those parameters and multiple responses was derived via response surface methodology (RSM). As a result, the optimal extraction condition for maximizing phenolic content and antioxidant activity was determined to be agitation at 109.54 rpm and a solid loading of 172.67 g/L. Under optimal conditions, SBB extract showed a total phenolic content of 0.21 mg/mL and ABTS and DPPH activities of 27.27% and 58.16%, respectively. The SBB extract prepared under optimal conditions was found to contain caffeic acid, vanillic acid, rutin, and vitamin B1 (thiamine). This work is the first challenge to design an optimization model for the efficient recovery of antioxidants from SBB and is significant in that the model can be applied simply and economically to conventional extraction processes. [ABSTRACT FROM AUTHOR]

This study investigated the effects of microencapsulating Myrtus communis fruit extracts within intact and plasmolysed Saccharomyces cerevisiae yeast cells on the phenolic compounds and antioxidant capacity of the extracts. Spectroscopic techniques were employed to quantify polyphenols, flavonoids, and tannins, while UPLC-ESI-MS-MS analysis was utilized to identify and quantify phenolic compounds before and after microencapsulation. Molecular docking was conducted to assess the interaction between yeast cell components and the major identified compounds. Microencapsulation was found to result in a diminished content of polyphenols, flavonoids, and tannins. Phenolic compounds showed higher polyphenol content when encapsulated in plasmolysed cells compared to non-plasmolysed yeast cell. Three major compounds were detected in the microcapsules: myricetin, chlorogenic acid, and coumaric acid, with myricetin exhibiting the highest level of interaction with target proteins. FTIR analysis revealed the interaction between phenolic compounds and yeast cell components, while SEM showed aggregation of microcapsules within plasmolysed microcapsules. Encapsulation of myrtle extract in yeast cells preserved cell wall integrity and microcapsule morphology, with superior thermal stability of encapsulated extracts compared to their non-encapsulated counterparts as demonstrated by TGA–DSC analysis. An enhancement in antioxidant activity after encapsulation was also observed, with the highest activity in plasmolysed yeast cell microcapsules. [ABSTRACT FROM AUTHOR]

In this work, the antioxidant components in persimmon (Diospyros kaki) leaves were separated by offline two‐dimensional liquid chromatography‐electrochemical detection (LC×LC‐ECD) and identified by LC‐tandem mass spectrometry (LC‐MS/MS). A total of 33 antioxidants, mainly proanthocyanidins, and glycosides of kaempferol and quercetin, were identified. The antioxidant assays demonstrated that the fractions collected from the first‐dimension LC (1D‐LC) possessed considerable radical scavenging capabilities, with correlation coefficients of peak area versus radical scavenging capability of 1,1‐diphenyl‐2‐picrylhydrazyl and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid) being 0.9335 and 0.9116, respectively. The fingerprinting showed that 37 peaks were present in all samples. The major antioxidant components of persimmon leaves were the glycosides of kaempferol and quercetin. Finally, fourteen antioxidants were quantitatively assessed. Offline LC×LC provided high peak capacity and separation; ECD enabled specific screening and detection of antioxidant components; and MS/MS provided excellent identification capability. In this study, the combination of the three approaches was utilized to screen for antioxidant components in persimmon leaves, with satisfactory findings. In conclusion, this technique is an effective means for rapid analysis of antioxidant components and quality control of medicinal plants, achieving rapid separation of congeners and facilitating more accurate qualitative and quantitative analyses. [ABSTRACT FROM AUTHOR]

Abstract Glehnia littoralis, a medicinal herb employed in traditional practices for alleviating fatigue, cough, and a dry throat, is recognized for its beneficial properties due to a diverse array of active compounds found in its extracts. For example, the G. littoralis roots (Radix Glehniae) mainly contain coumarins and phenolic acids, serving as the primary focus of this study. Despite the widespread use of the tools in various industries and the development of multiple analytical methods for their examination, the edible aerial parts have industrial potential, and there is currently no analytical method available to identify their key components. In this study, a high-performance liquid chromatography method combined with diode array detection (HPLC–DAD) was developed to simultaneously detect 16 phenolic compounds previously reported to be present in the edible aerial parts of G. littoralis. The proposed approach included using gradient elution to change the solvent system from water/acetonitrile to water/methanol. Furthermore, the method validation was conducted, assessing its linearity, limit of detection, limit of quantification, precision, accuracy, and recovery, all of which demonstrated satisfactory results. Subsequently, the developed method was applied to quantify the phenolic compounds in various G. littoralis samples obtained from different organs, solvent extraction processes, and processing methods. Moreover, the online HPLC-ABTS (2,2ʹ-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assay was used to evaluate the antioxidant capacities of individual constituents, identifying four important antioxidants and estimate the overall antioxidant capacity of the G. littoralis extract.

Glehnia littoralis, a medicinal herb employed in traditional practices for alleviating fatigue, cough, and a dry throat, is recognized for its beneficial properties due to a diverse array of active compounds found in its extracts. For example, the G. littoralis roots (Radix Glehniae) mainly contain coumarins and phenolic acids, serving as the primary focus of this study. Despite the widespread use of the tools in various industries and the development of multiple analytical methods for their examination, the edible aerial parts have industrial potential, and there is currently no analytical method available to identify their key components. In this study, a high-performance liquid chromatography method combined with diode array detection (HPLC–DAD) was developed to simultaneously detect 16 phenolic compounds previously reported to be present in the edible aerial parts of G. littoralis. The proposed approach included using gradient elution to change the solvent system from water/acetonitrile to water/methanol. Furthermore, the method validation was conducted, assessing its linearity, limit of detection, limit of quantification, precision, accuracy, and recovery, all of which demonstrated satisfactory results. Subsequently, the developed method was applied to quantify the phenolic compounds in various G. littoralis samples obtained from different organs, solvent extraction processes, and processing methods. Moreover, the online HPLC-ABTS (2,2ʹ-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) assay was used to evaluate the antioxidant capacities of individual constituents, identifying four important antioxidants and estimate the overall antioxidant capacity of the G. littoralis extract. [ABSTRACT FROM AUTHOR]

The main physiological and biochemical characteristics and elemental composition of three lichen species of the genus Hypogymnia (Nyl.) Nyl. in one habitat were studied using spectroscopic methods. The model species were placed in the following order of decreasing degree of anthropotolerance: H. physodes (L.) Nyl. → H. tubulosa (Schaer.) Hav. → H. vittata (Ach.) Parrique. The contents of chlorophylls a and b, phenolic compounds, pheophytinization quotient, and antiradical activity were determined by a spectrophotometric method. The antioxidant activity was determined by an amperometric method. The physiological and biochemical parameters for each of the three species corresponded to those for background ecotopes. These parameters and the integrity of the system of correlations between the parameters were lower in species with a low degree of anthropotolerance. Twenty-three elements were found in thalli of the model species using atomic emission spectroscopy with inductively coupled plasma. They included macro- and microelements, heavy metals, and metalloids. The maximum concentrations of most elements were found in H. vittata; the minimum concentrations, in H. physodes. An analysis of the interaction between the physiological and biochemical characteristics and the contents of the elements indicated the presence of a complex system of correlations in each species. Differences in this system of correlations may have been due to the specific composition of secondary metabolites, which determine the features of adaptive reactions. The use of various optical spectroscopy methods enabled an evaluation of not only the functional specificity of the studied species but also its connection to their anthropotolerance level. Low resistance to anthropogenic influences was combined with lower coordination of physiological and biochemical characteristics and low integrity of the system of correlations. The most vulnerable species H. vittata had the minimal values of the main functional parameters, a poorer correlation of them with the elemental composition, and higher concentrations of some toxic elements. The use of a complex analysis of the physiological and biochemical characteristics and elemental compositions using various spectral methods was crucial for the bioindication and ecological physiology of lichens. [ABSTRACT FROM AUTHOR]

Sabah is the main seaweed producer in Malaysia especially red seaweeds which are commercially cultivated for the production of food gum known as carrageenan. Seaweeds are also high in phytochemical such as antioxidant compounds. Three seaweeds species from the Semporna Seawater, Sabah, namely Kappaphycus alvarezii, Caulerpa lentillifera and Sargassum polycystum were chosen for this study for the analysis of their antioxidant activities. K. alvarezii species is commercially cultivated whereas the other two (C. lentillifera and S. polycystum) are wild species. All seaweeds species underwent drying process in a cabinet dryer prior to the analyses. Six types of solvents which were water, ethanol, methanol, chloroform, ethyl acetate and hexane were used for the extraction process. Every concentrated, extracted solutions were then measured for their antioxidant activities based on total phenolic content (TPC), diphenylpicrylhydrazyl (DPPH) radical scavenging activity, ferric reducing antioxidant power (FRAP) and β-carotene bleaching assay. Results showed that TPC of S. polycystum was significantly higher (p<0.05) than K. alvarezii but not significantly different (P>0.05) from C. lentillifera. Even though the extraction yield of water was the highest, but the greatest antioxidant activity values were seen in methanolic extraction in comparison to the other solvents in TPC, DPPH and FRAP. This indicated that most of the antioxidant compounds in all the three seaweed samples are polar. However, high antioxidant activity in terms of β-carotene bleaching assay were seen in other solvent extractions, which were chloroform, hexane and ethyl acetate. This indicated that seaweed also contain non-polar antioxidant compounds such as β-carotene. Overall, the best extraction method of antioxidant compounds in seaweed samples was using methanol as the extractant. [ABSTRACT FROM AUTHOR]

Phenolic compounds are the predominant chemical constituents in the secondary metabolites of plants and are commonly found in pears. In this study, we focused on the analysis of the phenolic composition and antioxidant activity of leaves from five pear cultivars (Cuiguan, Chaohong, Kuerle, Nanguoli, and Yali) and tea leaves (Fudingdabai as the control) using ultra-performance liquid chromatography coupled with electrospray ionization triple quadrupole mass spectrometry. The results indicated significant differences in the amount and composition of phenolic metabolites between tea and pear leaves, as well as among the five pear varieties. Only approximately one-third of the metabolites exhibited higher levels in pear leaves compared to that in tea leaves. The total phenol content in the Yali cultivar was higher than that in the other pear cultivars. Furthermore, specific phenolic metabolites with high expression were identified in the leaves of different groups. The levels of delphinidin 3-glucoside, aesculin, prunin, cosmosiin, quercetin 3-galactoside, isorhamnetin-3-O-glucoside, nicotiflorin, narcissin, chlorogenic acid, and cryptochlorogenic acid were relatively high among the five pear cultivars. (-)-Gallocatechin gallate, 6-methylcoumarin, aesculetin, hesperidin, kaempferol, and caftaric acid were identified as specific metabolic substances unique to each type of pear leaf. Most of the differential metabolites showed positive correlations and were primarily enriched in the flavonoid biosynthesis, flavone and flavonol biosynthesis, and phenylpropanoid biosynthesis pathways. DPPH (1,1-Diphenyl-2-picrylhydrazyl radical) analysis indicated that the Yali cultivar exhibited the highest antioxidant activity compared to other varieties. This systematic analysis of the differences in phenolic metabolite composition and antioxidant activity between pear and tea leaves provides a theoretical foundation for the development and utilization of pear leaf resources.

Sea buckthorn berries (SBB) are well known for being rich in natural bioactive compounds with high pharmacological activity. In this study, the optimization of extraction parameters was performed to recover phenolic compounds with high antioxidant activity from SBB. This study involved a systematic optimization approach, including screening for a variety of parameters, including temperature, time, ethanol concentration, agitation, and solid loading. On the basis of Plackett–Burman design (PBD) model, the two most significant parameters (agitation and solid loading) were selected, and the correlation model between those parameters and multiple responses was derived via response surface methodology (RSM). As a result, the optimal extraction condition for maximizing phenolic content and antioxidant activity was determined to be agitation at 109.54 rpm and a solid loading of 172.67 g/L. Under optimal conditions, SBB extract showed a total phenolic content of 0.21 mg/mL and ABTS and DPPH activities of 27.27% and 58.16%, respectively. The SBB extract prepared under optimal conditions was found to contain caffeic acid, vanillic acid, rutin, and vitamin B1 (thiamine). This work is the first challenge to design an optimization model for the efficient recovery of antioxidants from SBB and is significant in that the model can be applied simply and economically to conventional extraction processes.

Puerarin, which was used as a natural isoflavone in this study, was obtained from the Kudzu plant. The inhibitory effect of puerarin on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes was investigated. The IC50 values of AChE (177.73 nM) and BChE (315.07 nM) were determined. The binding mechanism of puerarin, which has a high inhibitory effect on the active site of the enzyme, was demonstrated by the molecular docking method. The antioxidant activity was evaluated for the same molecule. For this purpose, Fe3+‐Fe2+ reducing capacity, ABTS radical scavenging activity, CUPRAC method, DPPH⋅ scavenging activity and DMPD radical scavenging method were used. The percentage (%) inhibition amount of puerarin molecule was calculated as 99.33 % for ABTS radical scavenging activity, which is the more preferred method for natural products. The absorbtion value of iron reduction of Puerarin was 0.122±0.010, and the value of copper reduction was 0.633±0.026. Our research efforts include uncovering the potent antioxidant potential of puerarin and, simultaneously, a comparative study of tacrine, primarily emphasizing its inhibitory effects against AChE and BChE enzymes. The puerarin is expected to be a guide for drug discovery against many problems, especially oxidative cell damage in Alzheimer's disease, mitochondrial dysfunction (MD), cancer, and cardiovascular diseases. [ABSTRACT FROM AUTHOR]

Pink-pigmented facultative methylotrophs bacteria are a plant's surface inhabitant, especially at the leaf. They are known as Methylobacterium species. The antioxidant activity, phenolic compounds, and level of toxicity of this bacteria pigment have been studied. Recently, no previous research focused on the same bacterium found in Melicope lunu-ankenda (Gaertn.) T. G. Hartley, which is a component of the Malaysian ulam leaf. This study employed the 2,2-diphenyl-1-picrylhydrazyl (DPPH), ferric ion reducing antioxidant power (FRAP), and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays, along with total phenolic content determination to assess the antioxidant activities of the methanolic and ethanolic pigment extract. Additionally, the consumption safety level of the pigment extract used brine shrimp lethality assay. From these findings, ethanolic pigment extract has a higher antioxidant capacity than methanolic extract. The DPPH half-maximal inhibitory concentration (IC50) value of methanolic pigment extract is higher than ethanolic extract (0.72 ± 0.04 mg/ml), but the IC50 value is vice versa for ABTS (4.59 ± 2.17 mg/ml). Furthermore, ethanolic extracts have a high FRAP assay value (1.09 ± 0.19 mg/mg of trolox equivalent at 0.78 mg/ml sample) and phenolic content (1.39 ± 0.07 mg/mg of gallic acid equivalent at 0.78 mg/ml sample) compared to methanolic pigment extracts. Fortunately, the methanolic and ethanolic pigment extract's lethal concentration values (4.52 and 9.94 mg/ml) are considered safe for food application since their toxicity level is higher than 1 mg/ml. [ABSTRACT FROM AUTHOR]

'Abate Fétel', a winter cultivar, is the most important pear cultivar in Italy; its fruits are appreciated by consumers for their aroma, texture and balanced sweet and sour taste. Maintaining high-quality characteristics to prolong the shelf-life of fruit and preserve the sensory and nutritional quality is a priority for the food industry. The aim of our study was to test the effectiveness of 1-methylcyclopropene (1-MCP) and cold storage in prolonging the shelf-life of these fruits, which were harvested at maturity at two different times. This work focused on the effects of different storage treatments and two ripening times on (i) the chemical composition of Abate Fétel pulp fruits to preserve their sweet taste and aroma and (ii) the phenolic profile composition and antioxidant activity of the peel, which is naturally rich in phytochemicals and important for the fruit's shelf-life and in the functional food industry for its high nutritional value. Abate Fétel fruits were harvested at the optimal commercial maturity stage, first on 15 September, having been treated with 1-MCP and stored for 2 months at cold temperatures; the other fruits were harvested at the end of September and stored in a cold cell for 2 months. The fruit pulp was tested for glucose and fructose, pH, acidity and organic acids (malic, citric, fumaric and shikimic), phenolic content and phenolic compounds (chlorogenic and caffeic acids, rutin, hyperoside, kaempferol-3-rutinoside and isoquercitrin), and the antioxidant activities in the fruit peels were measured. Treating the fruits with 1-MCP better preserved the phytochemical compounds compared to simple refrigeration, preserving the fruit's quality and prolonging its shelf-life. All the treatments help to maintain the glucose and fructose content and the acidity, preserving the aroma and organoleptic characteristics. [ABSTRACT FROM AUTHOR]

Hypertension (HTN) is one of the leading causes of death in the world. Agri-food by-products are emerging as a novel source of natural antihypertensive agents allowing for their valorization and making food and agricultural industries more environmentally friendly. In this regard, wine making process generates large amounts of by-products rich in phenolic compounds that have shown potential to exert several beneficial effects including antihypertensive properties. The aim of this study was to review the blood pressure-lowering effects of winery by-products. In addition, molecular mechanisms involved in their bioactivity were also evaluated. Among the winery by-products, grape seed extracts have widely shown antihypertensive properties in both animal and human studies. Moreover, recent evidence suggests that grape stem, skin and pomace and wine lees may also have great potential to manage HTN, although more studies are needed in order to confirm their potential in humans. Improvement of endothelial dysfunction and reduction of oxidative stress associated with HTN are the main mechanisms involved in the blood pressure-lowering effects of these by-products. [ABSTRACT FROM AUTHOR]

Smart farming is a promising strategy for future agriculture, and the global market for smart agriculture is growing rapidly. The aim of this study was to compare the quality of agricultural products based on their cultivation method. The components and antioxidant activities of Lactuca sativa (LS) var. longifolia cultivated using conventional agriculture methods (CAMs) and smart farm agriculture methods (SAMs) were compared. ABTS+ and DPPH radical scavenging activity assays were performed to compare the antioxidant activities of LS grown using CAMs and SAMs. High-performance liquid chromatography with a reverse-phase column was employed to analyze the phenolic and carotenoid compounds. In both the SAM and the CAM, β-carotene was found to be the most abundant compound. LS grown using the CAM had high total polyphenol content and ABTS+ radical scavenging activity, while LS grown using the SAM had high individual phenolic content and DPPH radical scavenging activity. This study developed a new method of SAM and confirmed that SAM produces a higher quantity of phenolic compounds and total flavonoid contents than CAM in LS. This study also highlights the SAM's functioning, gives crop quality inspection guidelines, and standardizes LS. The new SAM is effective for application in undeveloped urban spaces, is more convenient than the CAM, and represents a way to develop and manage LS quality. [ABSTRACT FROM AUTHOR]

The green pepper has garnered interest in different societies as a functional food and food additive. Numerous studies have indicated that the phytochemicals found in pepper possess antioxidant, anti-cancer, anti-inflammatory, anti-obesity, and anti-arteriosclerotic properties. In this study, green pepper extract was used in yogurt to increase the acceptability of consumption and improve its health properties. For this purpose, green pepper extract was added in concentrations 100, 200, and 300 ppm in the preparation of yogurt. And pH, acidity, total content of phenolic compounds, DPPH inhibition percentage, viscosity, syneresis, and sensory properties were evaluated in 1, 7, 14, and 21 days after production. The results showed that by increasing the amount of extract, the percentage of antioxidant activity and phenolic compounds increased significantly. The effect of storage time on these indicators was also significant (p

Abstract Perilla frutescens is a medicinal herb that is commonly cultivated in Asian countries. Perilla seed is extensively pressed for cooking oil extraction. However, phenolic chemicals are still abundant in pressed perilla seed meal (PSM), which was previously thought to be useless after oil extraction. In our study, PSM was extracted using five solvents (water and 25%, 50%, 75%, and 100% ethanol) based on different ethanol concentrations, and its antioxidant activity, phenolic compounds, and inhibitory effects against key enzymes related to diabetes mellitus were evaluated. The 75% ethanol extract had higher phenolic (105.58 mg GAE/g DW) and flavonoid (66.52 mg QE/g DW) contents and showed better antioxidant and inhibitory effects against α‐glucosidase and α‐amylase. Analysis of the phenolic compounds of the five extracts by HPLC indicated the presence of apigenin, rosmarinic acid, benzoic acid, caffeic acid, and vanillic acid. Therefore, because of its high antioxidant activity and inhibitory capacity against enzymes relevant to diabetes, the 75% ethanol extract of perilla seed meal has the most potential to be used as a functional or nutraceutical food in the prevention and treatment of oxidation and diabetes.

Abstract Chinese wild rice (Zizania latifolia) is rich in phenolic compounds, particularly flavonoids. This study identified 203 basic helix‐loop‐helix (bHLHs) in Z. latifolia and showed that ZlbHLH196 (Zla16G011250) corresponds to the ZlRc gene in Z. latifolia, with its protein product localizing to the nucleus. Notably, the pericarps of ZlRc‐overexpressing (OE) rice are brown, whereas those of wild‐type (WT) rice are nonpigmented. The total phenolic, flavonoid, and proanthocyanidin contents, antioxidant activity, as well as enzyme inhibitory effects of ZlRc‐OE rice were significantly higher than those of WT rice. Overall, 221 differential phenolic metabolites were identified between ZlRc‐OE and WT rice, among which 198 were upregulated in the former. Additionally, a total of 227 differentially expressed genes were identified between ZlRc‐OE and WT rice, with 173 upregulated. Kyoto Encyclopedia of Genes and Genomes annotation and enrichment analysis of phenolic metabolites revealed enhanced isoflavonoid, flavone, flavonol, and flavonoid biosynthesis pathways in ZlRc‐OE rice, which, furthermore, showed a markedly upregulated expression and significantly higher activities of four key flavonoid biosynthesis–related enzymes (phenylalanine ammonia‐lyase, chalcone synthase, chalcone‐flavanone isomerase, and dihydroflavonol 4‐reductase). These findings show that ZlRc‐overexpression promotes phenolic compound accumulation in rice seeds and can be used to bioaugment rice phenolic content.

Perilla frutescens is a medicinal herb that is commonly cultivated in Asian countries. Perilla seed is extensively pressed for cooking oil extraction. However, phenolic chemicals are still abundant in pressed perilla seed meal (PSM), which was previously thought to be useless after oil extraction. In our study, PSM was extracted using five solvents (water and 25%, 50%, 75%, and 100% ethanol) based on different ethanol concentrations, and its antioxidant activity, phenolic compounds, and inhibitory effects against key enzymes related to diabetes mellitus were evaluated. The 75% ethanol extract had higher phenolic (105.58 mg GAE/g DW) and flavonoid (66.52 mg QE/g DW) contents and showed better antioxidant and inhibitory effects against α‐glucosidase and α‐amylase. Analysis of the phenolic compounds of the five extracts by HPLC indicated the presence of apigenin, rosmarinic acid, benzoic acid, caffeic acid, and vanillic acid. Therefore, because of its high antioxidant activity and inhibitory capacity against enzymes relevant to diabetes, the 75% ethanol extract of perilla seed meal has the most potential to be used as a functional or nutraceutical food in the prevention and treatment of oxidation and diabetes. [ABSTRACT FROM AUTHOR]

This study assessed the extraction of phenolic compounds from germinated deoiled flaxseed flour using ultrasound and response surface methodology. For this purpose, a central composite design with independent variables of time (15, 30, and 45 min) and temperature (20, 40, and 60 °C) were used. Several antioxidant activity tests were also carried out. Independent variables significantly affected the extraction of phenolic and flavonoid compounds. Germinated deoiled flaxseed flour extract contains the highest levels of phenolic and flavonoid compounds after being ultrasound at 40 °C for 30 min. The anti-oxidant, antiarthritic, and ACE inhibitory activities of germinated deoiled flaxseed flour extract were higher when using the ultrasound at 40 °C for 30 min than with solvent extraction. Different phenolic and flavonoid compounds were identified in germinated deoiled flaxseed flour extract obtained from ultrasound-assisted extraction. [ABSTRACT FROM AUTHOR]

Legumes are a rich source of bioactive compounds such as phenolic or polyphenolic compounds, particularly tocopherols. Legumes antioxidants are widespread for their radical scavenging proprieties as active biologic compounds belonging to various chemical classes. Polyphenols are the most studied molecules of both nutritional and pharmaceutical interest. Furthermore, an overview concerning the antioxidant capacity and determination is mandatory for the precise and accurate method selection, involving cost-effectiveness and time-saving, towards gathering networks between research and development fields. The current review aims to summarize the presence of the natural antioxidant, their multiple biological effects, and the various approaches to methods determinations. [ABSTRACT FROM AUTHOR]

The complex structure of rice straw is such that its bioconversion requires multiple physical and chemical pretreatment steps. In this study, it was found that a large amount of soluble polysaccharides (SPs) are formed during the pretreatment of straw. The yield of NaOH-based SPs (4.8%) was much larger than that of ball-milled SPs (1.5%) and H2SO4-based SPs (1.1%). For all the pretreatments, the ratio of phenolic compounds to saccharides (P/S) for each type of SPs increased upon increasing the concentration of ethanol in the order of 90% > 70% > 50%. The yield of NaOH-based SPs was much higher than that of acid-based and ball-milled SPs. The changes in the 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS), ferric reducing antioxidant power assay (FRAP), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) of SPs follow the same rule, i.e., the higher the P/S ratio, the higher the antioxidant values of the SPs. The flow cytometry and laser scanning microscopy results show that the P/S ratio can significantly influence the effect of SPs on microbial growth and cell membrane permeability. Upon varying the ethanol concentration in the range of 50–90%, the P/S ratio increased from 0.02 to 0.17, resulting in an increase in the promoting effects of the SPs on yeast cell growth. Furthermore, H2O2, NAD+/NADH, and NADP+/NADPH assays indicate that SPs with a high P/S ratio can reduce intracellular H2O2 and change the intracellular redox status. [ABSTRACT FROM AUTHOR]

Table grapes have a high-water content and are non-climacteric. They are prone to degradation and infections. This study reveals the effect of neem seed oil combined with methylcellulose (NSOMC) at (control, 0.5%NSOMC, 2%NSOMC, 8%NSOMC, 32%NSOMC and 1% of methylcellulose (MC) prolonging the postharvest quality of Sultana table grapes during 60 days of storage at 0.5°C. The different concentrations of the NSOMC were found effective compared to the control and 1%MC. NSOMC coated fruits, especially at 32% showed less decay incident (3.1%), weight loss (0.32%) and colour changes (8.49) compared to the other coated fruits and the control. 32%NSOMC coated fruits retained their total soluble content and titratable acidity with the lowest mean pH value (3.43). Phenolic compounds (total phenolic and total flavonoid content) had the highest mean and the antioxidant activity was reduced in 32%NSOMC coated fruits. Therefore, our findings suggested that the neem seed oil combined with methylcellulose is safe and could be used as a commercial treatment to increase the shelf life of table grapes. The higher the concentrations of the neem seed oil, the greater the efficiency of the treatments. [ABSTRACT FROM AUTHOR]

Abstract In this study, optimization of the extraction of phenol compounds from Mentha piperita using ultrasonic waves with response surface methodology (RSM) was assessed. In this regard, a central composite design with three independent variables of time (5, 27.5, and 50 min), temperature (25, 45, and 65°C), and concentrations of ethanol in the water–ethanol solution (0%, 50%, and 100%) was used. Besides, the antioxidant activity tests (DPPH radical scavenging assay, ferric reducing antioxidant power [FRAP], and oxidative stability indexes [OSI]) were examined. Significant effects of independent variables on the extraction of phenol compound, DPPH radical scavenging power, and OSI of M. piperita extract, with the regression coefficients of 0.89, 0.92, and 0.94, respectively, were noted. However, no significant difference in terms of the FRAP among different treatments was noted. Also, the best antioxidant activity of M. piperita was obtained by using the ultrasonic wave for 50 min at 65°C and 59.6% v/v ethanol/water solution. While the findings of RSM confirmed the experimental results, due to the favored properties of M. piperita extract by the proposed method, further research to investigate possible applications in the food industry is recommended.

Peanut sprout is a high-quality healthy food, which not only has beneficial effects, but also a higher phenol content than peanut seed. In this study, peanut sprout was treated with five cooking methods, namely boiling, steaming, microwave heating, roasting, and deep-frying, and the phenol content, monomeric phenol composition, and antioxidant activity were determined. The results showed that, compared with unripened peanut sprout, the total phenol content (TPC) and total flavonoid content (TFC) decreased significantly after the five ripening processes, and the highest retention of phenols and flavonoids was associated with microwave heating (82.05% for TPC; 85.35% for TFC). Compared with unripened peanut sprout, the monomeric phenol composition in germinated peanut was variable after heat processing. After microwave heating, except for a significant increase in the cinnamic acid content, no changes in the contents of resveratrol, ferulic acid, sinapic acid, and epicatechin were observed. Furthermore, there was a significant positive correlation of TPC and TFC with 2,2-diphenyl-1-picrylhydrazyl scavenging capacity, 2,2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) scavenging capacity, and ferric ion reducing antioxidant power in germinated peanut, but not with hydroxyl free radical scavenging capacity, in which the main monomer phenolic compounds were resveratrol, catechin, and quercetin. The research results indicate that microwave heating can effectively retain the phenolic substances and antioxidant activity in germinated peanuts, making it a more suitable ripening and processing method for germinated peanuts. [ABSTRACT FROM AUTHOR]

The effects of pre- and postharvest calcium gluconate (Ca-Glu) treatments on some physicochemical characteristics and bioactive compounds of sweet cherry cv. Sweetheart during cold storage were investigated. For preharvest treatments, the Ca-Glu (1%) solution was applied to the cherry trees two times at 21 and 35 days after full bloom stage. Control trees were sprayed with distilled water at the same days. Sweet cherries, sprayed with and without Ca-Glu, were dipped into cold water (4 °C) containing calcium gluconate (1%) for 30 s and only in cold water (4 °C) as control, after harvest Following each treatment, cherries were placed in plastic boxes and stored at 1 ± 0.5 °C and 90 ± 5% relative humidity for 3 weeks. The weight losses of cherries increased over time but calcium (Ca) treatments, especially pre-and postharvest combination, limited these increases compared to control groups. The best result for suppressing the respiration rate of cherries was also obtained from combined treatment. Moreover, combined treatment delayed the losses of titratable acidity, fruit firmness, decay rate and sensory quality in sweet cherries during storage comparison with the pre or postharvest application of Ca-Glu alone. The effect of Ca-Clu treatments on stem chlorophyll content and antioxidant activity was not significant. Preharvest and combined treatments retarded the loss of ascorbic acid content of cherries compared to postharvest and control treatments. The total phenolic and anthocyanin content increased regularly throughout storage, regardless of treatment; however, Ca treatments delayed the accumulation of these compounds. As a result, the combined Ca-Glu treatment could be a promising method for maintaining some physicochemical characteristics and bioactive compounds in sweet cherries during cold storage. [ABSTRACT FROM AUTHOR]

The fresh in-hull pistachio fruit was sprayed with 1% calcium oxide (CaO), 10 mM w/v γ-aminobutyric acid (GABA), 1% carboxymethyl cellulose (CMC), and their combination (CMC+GABA+CaO) for 30 sec at room temperature. Spraying with distilled water was considered as a control. Following drying of fruits at room temperature, they were packed in perforated polyethylene terephthalate containers and kept under cold storage (at 2 ± 1 °C with 85 ± 2% RH) for 75d. Pistachios treated showed the lower weight loss, and the highest hull safety, taste, aroma, and overall acceptance than control. Total chlorophyll and anthocyanin of fresh pistachio kernel were preserved by combined treatment. Combined treatment reduced the PPO, POD, PAL activity and MDA content of the hull and maintained kernel phenolic compounds compared to other treatments. Besides these, the best effects were obtained with combined treatment, which maintained the physical, biochemical and visual characteristics of the fresh fruit for 50 days. [ABSTRACT FROM AUTHOR]

Objective: Heliciopsis terminalis (Kurz.) Sleum (family Proteaceae) is distributed in India, Mianma, Thailand, Cambodia, Vietnam, and China. In Vietnamese traditional medicine, it is used as an antidote for detoxifying the liver and detoxifying alcohol, a contraceptive, and to treat infections. The purpose of this study is to determine the chemical constituents of the plant, as well as their in vitro hepatoprotective and antioxidant activities. Methods: The dried powdered sample was ultrasonically extracted with MeOH, and the extract was fractionated by various chromatographic methods, including high-performance liquid chromatography. The structures of the isolated compounds were identified by NMR, high-resolution electrospray ionization mass spectrometry, and CD spectral analysis, and then screened for their in vitro hepatoprotective and antioxidant activities. Results: One new phenolic glycoside, named helitermioside (1), and eight known compounds (2-9) were isolated from the trunk and branches of H terminalis. All of the isolates were reported from H terminalis for the first time. Compound 6 showed antioxidant activity in thiobarbituric acid reactive substances (TBARS) lipid peroxidation inhibition assays, with an IC50 value of 61.16 ± 1.17 µg/mL, compared to that of Trolox (positive control), IC50 = 7.06 ± 0.11 µg/mL. At a concentration of 100 µg/mL, none of the isolates exhibited hepatocellular protective activity. Conclusion: Nine phenolic compounds (1–9) were recorded from H terminalis. Of these, compound 1 was new. Compound 6 exhibited moderate antioxidant activity in TBARS lipid peroxidation inhibition assays, but none of the compounds exhibited hepatocellular protective activity against CCl4-induced toxicity. [ABSTRACT FROM AUTHOR]

This paper investigates the effects of storage conditions on the stability of phenolics and their antioxidant activities in unique nutraceutical supplements containing non-traditional cereal flakes, edible flowers, fruits, nuts, and seeds. Significant total phenolic content (TPC) of 1170–2430 mg GAE/kg and total anthocyanin content (TAC) with the values of 322–663 mg C3G/kg were determined with the highest TPC content established in free phenolic fractions. The most notable declines in TPC (by 53%), TAC (by 62%), phenolics (e.g., glycosylated anthocyanins by 35–67%), and antioxidant activity (by 25% using DPPH) were established in the presence of sunlight at 23 °C followed by the storage at 40 °C. Quercetin, rutin, peonidin, pelargonidin, p-coumaric, ellagic, and p-hydroxybenzoic acids were identified as the least stable phenolics when exposed to sunlight. Furthermore, glycosylated forms of anthocyanins demonstrated a greater stability when compared with anthocyanidins. The mixtures considerably eliminated ABTS and DPPH radicals. In all samples, water-soluble substances showed a higher antioxidant activity than lipid-soluble substances with the main contributors in the following order: delphinidin-3-glucoside (r = +0.9839) > p-coumaric > gallic > sinapic > p-hydroxybenzoic acids > delphinidin > peonidin and malvidin (r = +0.6538). Gluten-free nutraceutical mixtures M3 (containing red rice and black quinoa flakes, red and blue cornflowers, blueberries, and barberries) and M4 (containing red and black rice flakes, rose, blue cornflower, blueberries, raspberries, and barberries) were evaluated as the least stable under all storage conditions although they showed considerable phenolic concentrations. Phenolic contents and antioxidant activity of the nutraceutical mixtures were the highest at 23 °C without the presence of sunlight with the most stable M1 nutraceutical mixture (containing oat and red wheat flakes, hibiscus, lavender, blueberries, raspberries, and barberries). [ABSTRACT FROM AUTHOR]

Emerging high-phenolic sweeteners impart a sweet taste to foods and beverages, and are desirable sugar alternatives. Most refined sugars have a low antioxidant content due to polyphenol degradation occurring during sugar refining. Natural sweeteners such as honey, molasses, and dark brown sugar possess moderate to high phenolic content. Other phytochemicals found in natural sweeteners are carotenoids, organic acids, and terpenoids. Additionally, molasses and syrups synthesised from anthocyanin-rich fruits and roots contain anthocyanins apart from flavonoids. Non-nutritive sweeteners, such as sugar alcohols, are low in calories besides their sweet taste. Sweet proteins, dihydrochalcones, phenolics, and terpenoid derivatives are emerging sweeteners. These sweet substances are effective antioxidants that could help reduce oxidative stress in the human body although the amount ingested is usually low. The present review emphasised specific natural, highphenolic, and other sweet compounds, and examined the antioxidative characteristics of these sweeteners. The risk of excessive ingestion of these sweet substances is yet to be proven. [ABSTRACT FROM AUTHOR]

Propolis is a complex natural compound that honeybees obtain from plants and contributes to hive safety. It is rich in phenolic and flavonoid compounds, which contain antioxidant, antimicrobial, and anticancer properties. In this study, the chemical composition and antioxidant activities of propolis were investigated; ABTS•+, DPPH• and DMPD•+ were prepared using radical scavenging antioxidant methods. The phenolic and flavonoid contents of propolis were 53 mg of gallic acid equivalent (GAE)/g and 170.164 mg of quercetin equivalent (QE)/g, respectively. The ferric ion (Fe3+) reduction, CUPRAC and FRAP reduction capacities were also studied. The antioxidant and reducing capacities of propolis were compared with those of butylated hydroxyanisole (BHA), butylated hydroxytoluene (BHT), α-tocopherol and Trolox reference standards. The half maximal inhibition concentration (IC50) values of propolis for ABTS•+, DPPH• and DMPD•+ scavenging activities were found to be 8.15, 20.55 and 86.64 μg/mL, respectively. Propolis extract demonstrated IC50 values of 3.7, 3.4 and 19.6 μg/mL against α-glycosidase, acetylcholinesterase (AChE) and carbonic anhydrase II (hCA II) enzyme, respectively. These enzymes' inhibition was associated with diabetes, Alzheimer's disease (AD) and glaucoma. The reducing power, antioxidant activity and enzyme inhibition capacity of propolis extract were comparable to those demonstrated by the standards. Twenty-eight phenolic compounds, including acacetin, caffeic acid, p-coumaric acid, naringenin, chrysin, quinic acid, quercetin, and ferulic acid, were determined by LC-MS/MS to be major organic compounds in propolis. The polyphenolic antioxidant-rich content of the ethanol extract of propolis appears to be a natural product that can be used in the treatment of diabetes, AD, glaucoma, epilepsy, and cancerous diseases. [ABSTRACT FROM AUTHOR]

In this study, optimization of the extraction of phenol compounds from Mentha piperita using ultrasonic waves with response surface methodology (RSM) was assessed. In this regard, a central composite design with three independent variables of time (5, 27.5, and 50 min), temperature (25, 45, and 65°C), and concentrations of ethanol in the water–ethanol solution (0%, 50%, and 100%) was used. Besides, the antioxidant activity tests (DPPH radical scavenging assay, ferric reducing antioxidant power [FRAP], and oxidative stability indexes [OSI]) were examined. Significant effects of independent variables on the extraction of phenol compound, DPPH radical scavenging power, and OSI of M. piperita extract, with the regression coefficients of 0.89, 0.92, and 0.94, respectively, were noted. However, no significant difference in terms of the FRAP among different treatments was noted. Also, the best antioxidant activity of M. piperita was obtained by using the ultrasonic wave for 50 min at 65°C and 59.6% v/v ethanol/water solution. While the findings of RSM confirmed the experimental results, due to the favored properties of M. piperita extract by the proposed method, further research to investigate possible applications in the food industry is recommended. [ABSTRACT FROM AUTHOR]

BACKGROUND: Food processing produces large volumes of waste that can be transformed into useful and relevant ingredients. The by‐product of oil extraction from faveleira seeds is a potentially low‐cost raw material that can be used for obtaining functional foods. This study aimed to analyze the physicochemical properties, chemical composition, and antioxidant activity of faveleira (Cnidoscolus quercifolius Pohl) seed press cake flour (FSPCF). Additionally, the chemical composition, antioxidant activity, and physical, microbiological, and sensory aspects of cookies with 0, 25%, or 50% substitution of refined wheat flour (RWF) with FSPCF (0‐FSPCF, 25‐FSPCF, and 50‐FSPCF cookies, respectively) were evaluated. RESULTS: FSPCF exhibited good physicochemical properties, high antioxidant activity (0.45 ± 0.00 and 42.83 ± 1.30 g TE g−1 for the DPPH and ABTS methods, respectively), and high polyphenol content (particularly gallic acid at 21015.85 ± 4981.76 g kg−1) and is also rich in minerals and fiber (359.40 ± 1.10 g kg−1). Replacement of RWF with FSPCF increased the activity of antioxidants and the levels of polyphenols, ash, lipids, proteins, and fibers. The 50‐FSPCF cookie possessed the highest linoleic acid content (97.50 ± 8.47 g kg−1). Flour replacement influenced the weight of the cookies without affecting the other physical characteristics. The cookies yielded good sensory acceptance and purchase intentions. Contamination was not detected. CONCLUSION: Faveleira flour possesses high nutritional and bioactive value and can be used as a functional ingredient in cookies and possibly in other bakery products such as bread, cakes, and pastas. © 2022 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

In this study, it was aimed to optimize the extraction of phenolic compounds from the madimak (Polygonum cognatum Meissn.), which is used in traditional folk medicine as well as nutrition. For this purpose, the effects of methanol/water mixture concentration (0-100%), extraction time (5-120 min.) and temperature (30-70 °C) on total phenolic compounds (TPC), total flavonoid compounds (TFC) and free radical scavenging activity (DPPH) of phenolic extracts obtained from P. cognatum were determined. Optimum conditions for TPC, TFC and DPPH values were determined using the "desirability" function approach, using the Box-Behnken design in the Design Expert Program. In the study carried out to obtain phenolic compound extraction from P. cognatum, methanol concentration (40.15%), extraction time (5.00 min.) and extraction temperature (70.00 °C) were determined as optimum conditions. TPC, TFC and DPPH values of phenolic extracts obtained under optimum conditions were determined as 45.40 mg GAE/g dry sample, 30.36 mg QE/g dry sample, 53.40 mg TE/g dry sample, respectively. As a result of the obtained results, P. cognatum is a rich source in terms of phenolic compounds and antioxidant activity. [ABSTRACT FROM AUTHOR]

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Abstract Sappan wood (Caesalpinia sappan L.) is a plant that contains many phytochemical constituents such as antioxidant, antimicrobial, antifungal and antiacne that have functional activities. Sappan woods have several phenolic compounds such as brazilin, xanthone, flavones and coumarin. In order to extract the phenolic compounds, Ultrasound-Assisted Extraction (UAE) is considered as green technology and gives better quality products with a higher extraction rate, shorter extraction time and less energy. The research investigated the effect of different ethanol concentrations on the sappan wood’s yield and studied the kinetic on its extraction process. Sappan wood was extracted using a type of UAE probe with ethanol concentrations of 50%, 60%, 70%, 80% and 90% (v/v). For the extract was analyzed its yield, Total Phenolic Content (TPC), antioxidant activity, Fourier Transform InfraRed (FTIR) spectroscopy and lees morphology. The kinetic study refers to Peleg’s model and was conducted for temperatures of 40 °C, 50 °C and 60 °C. The results showed that the increase of ethanol concentration increased the yield of extract until ethanol was 80% and decreased when the ethanol concentration is higher than 80%. While the TPC slowly decreased, when the ethanol concentration increased. Comparing the UAE and sohxlet methods, UAE gave a higher yield in a shorter time (10.33% in 20 min) than the Soxhlet method (9.67% in 180 min). All the extracts were categorized as high antioxidant material since the IC50 points were lower than 50 ppm. FTIR spectroscopy showed that UAE did not change the functional group of the extract, while Scanning Electron Microscope (SEM) pictures showed the more obvious cracked and deep holes of UAE’s lees than the Soxhlet’s lees. Peleg’s model was suitable to describe the extraction kinetics with R2 higher than 0.93. Regarding both yield and TPC, a mixture of ethanol-water with ethanol 60% (v/v) could be the optimum solvent concentration (10.33% yield with TPC 2.63 mg GAE/g) for UAE of sappan wood.

‘Abate Fétel’, a winter cultivar, is the most important pear cultivar in Italy; its fruits are appreciated by consumers for their aroma, texture and balanced sweet and sour taste. Maintaining high-quality characteristics to prolong the shelf-life of fruit and preserve the sensory and nutritional quality is a priority for the food industry. The aim of our study was to test the effectiveness of 1-methylcyclopropene (1-MCP) and cold storage in prolonging the shelf-life of these fruits, which were harvested at maturity at two different times. This work focused on the effects of different storage treatments and two ripening times on (i) the chemical composition of Abate Fétel pulp fruits to preserve their sweet taste and aroma and (ii) the phenolic profile composition and antioxidant activity of the peel, which is naturally rich in phytochemicals and important for the fruit’s shelf-life and in the functional food industry for its high nutritional value. Abate Fétel fruits were harvested at the optimal commercial maturity stage, first on 15 September, having been treated with 1-MCP and stored for 2 months at cold temperatures; the other fruits were harvested at the end of September and stored in a cold cell for 2 months. The fruit pulp was tested for glucose and fructose, pH, acidity and organic acids (malic, citric, fumaric and shikimic), phenolic content and phenolic compounds (chlorogenic and caffeic acids, rutin, hyperoside, kaempferol-3-rutinoside and isoquercitrin), and the antioxidant activities in the fruit peels were measured. Treating the fruits with 1-MCP better preserved the phytochemical compounds compared to simple refrigeration, preserving the fruit’s quality and prolonging its shelf-life. All the treatments help to maintain the glucose and fructose content and the acidity, preserving the aroma and organoleptic characteristics.

Extracts and their concentrates can be considered as a source of biologically active ingredients of food products and as food additives to increase the consumption of useful substances of berries. The benefits gained from their use lead to the development of advanced technologies that can allow them to be extracted from the feedstock without significantly compromising the biological activity of the feedstock. In this paper, we studied the content of antioxidants and their activity in concentrated extracts of cranberry (Vaccinium oxycoccus), sea buckthorn (Hippophae rhamnoides L.), blackberry (Rubus fruticosus), guelder rose (Viburnum opulus L.) and mountain ash (Sorbus aucuparia L.) berries. For all extracts, the total content of phenolic compounds, flavonoids and anthocyanins, antioxidant activity according to the DPPH method and iron-reducing antioxidant activity (FRAP) were determined. Concentrated extracts of guelder rose contain the largest amount of phenolic compounds (9.3 ± 0.3 mol HA/L) , flavonoids (1.96 ± 0.08 mol K/L) and anthocyanins (0.26 ± 0.02 mol CG/L) among the studied extracts. There is also a high total content of phenolic substances and flavonoids in blackberry and rowan extracts (5.7 mol HA/L, 1.33 mol K/L and 4.7 mol HA/L, 192 mol K/L, respectively). The antioxidant activity of the extract of guelder rose berries showed a directly proportional dependence on the content of biologically active compounds in it: DPPH-method, 2.4 mg/mL; FRAP-method, 39.99 mmol Fe2+/kg. Also, high levels were found in concentrated extracts of blackberries, sea buckthorn and mountain ash. It is the extracts of guelder rose, blackberry and mountain ash that are recommended to be used in the production of food products to enrich them with biologically active substances and give them antioxidant properties. Cranberry and sea buckthorn extracts can be used in combination with other extracts. [ABSTRACT FROM AUTHOR]

This study evaluated the antioxidant properties and kidney cell protection of medicinal plants as natural medicaments. A total of four medicinal plants including turmeric (TM), gangwha mugwort (GM), glasswort (GW), and omija (OM) were selected and fermented. Hot water extracts (HWE) and ethanol extracts (EE) of the plants were prepared before and after fermentation and tested in experiments in vitro and in vivo. Total polyphenol contents (TPC) and total flavonoid content (TFC) of GM were the highest among them. The TPC based HWE decreased after fermentation except OM; in contrast, TFC from HWE increased. The DPPH radical scavenging activity and ABTS value from HWE increased after fermentation, especially OM, which showed significant differences, while DPPH and ABTS from EE were decreased. The cell viability was not changed after addition of these plants extracts below 50 μg/mL; however, TM from HWE significantly decreased. The protective effect on kidney cells against cisplatin showed a 60% range of cell viability in each plant extract. In the in vivo experiment, the protective effect on kidney cells by the supplemented plant extracts was demonstrated by the serum creatinine and BUN level. During experimental periods, the serum creatinine and BUN level of GW and GM-treated mice decreased with significant differences compared to the adenine control group. As a result, these plant extracts had no cytotoxicity and maintained a protective effect as well as antioxidant activity. These results suggest that plants such as gangwha mugwort (GM) and glasswort (GW) may be good extracts for kidney cell protection and antioxidant agents. [ABSTRACT FROM AUTHOR]

The extraction of phenols from almond skin using water has not been applied before. The purpose of this study was to obtain aqueous extracts from almond skin to be added to pork patties to prolong their shelf life. Four different varieties of almonds were studied and aqueous extracts were obtained. The antioxidant capacity and composition of phenol compounds of the extracts were determined. Results showed that the use of water produces extracts with phenol compounds and antioxidant capacity, with the Antoñeta variety presenting the best performance in terms of antioxidant behavior. The most abundant phenolic compounds identified were isorhamentin-3-O-rutinoside, catechin and protocatechuic acid, all of them had a hydrophilic character due to the –OH groups in their molecules. The effect of almond skin extracts (ALMOND) on the shelf life of pork patties was compared with the effects of a control without extract (CONTROL NEG) and a control with sodium ascorbate (CONTROL POS). Throughout storage, values of pH, weight loss, headspace composition, color, TBARs and psychrotrophic aerobic bacteria were studied. CONTROL POS samples showed the lowest lipid oxidation values in comparison to CONTROL NEG or ALMOND extract samples. [ABSTRACT FROM AUTHOR]

A natural antioxidant extract obtained from oil mill wastewater was used for the formulation of basil pesto sauce, with the aim to improve quality and stability during storage. The antioxidant extract was added to traditional ingredients (basil, cheese, oil, etc.) and after preparation, packaging, and thermal treatment it was submitted to storage (monitored for 90 days). Fresh samples were stored at 4 °C and pasteurized samples were stored at room temperature. The effect of natural antioxidant addition on basil pesto sauce was evaluated for the main qualitative attributes, such as: physicochemical, microbiological, and antioxidant parameters. The principal results showed that the addition of a natural phenolic extract led to an evident reduction in pH, attaining food safety values under pH 4. The high oxidative stability observed in the basil pesto sauces fortified with the phenolic extract suggests that the incorporation of phenolic compounds delays the propagation phase of lipid oxidation. [ABSTRACT FROM AUTHOR]

The comparative phytochemical analysis in this study revealed differences in the type and levels of phenolic compounds between Spiraea hypericifolia L. and Spiraea crenata L. The compounds in water–ethanol extracts of aerial parts of both species were identified by high-performance liquid chromatography as chlorogenic, gentisic, and cinnamic acids; quercetin; kaempferol; hyperoside; isoquercetin; nicotiflorin; and apigenin. In the extract of S. hypericifolia, p-coumaric acid and luteolin were also found, which were absent in the extract of S. crenata. Such compounds as avicularin, astragalin, and isorhamnetin-3-rutinoside proved to be specific to S. crenata (and were not found in the S. hypericifolia extract). The viability of liver cancer HepG2 cells and breast cancer MDA-MB-231 cells significantly decreased after cultivation with the S. crenata extract. In addition, the S. crenata extract showed higher antioxidant activity than the S. hypericifolia extract. It is most likely that these effects can be explained by the higher content of individual flavonoids in the extract of S. crenata. Thus, the extract of S. crenata holds promise for more extensive research on the mechanism of its action on tumor cells. [ABSTRACT FROM AUTHOR]

In the study, bioactive component extraction was performed using the classical extraction method from broccoli. In the classical extraction method, the highest total phenolic substance (TPS), antioxidant activity (AOX) value and ascorbic acid content (AAC) determined were 690.94 mg GAE/kg dry matter (20°C/15min), 5.08 mg/ml (20°C/15 min) and 66.74 mg/100g dry matter (20°C/15 min), respectively. Extraction conditions were determined by using the Taguchi method. Accordingly, the best process conditions with the highest total phenolic substance, antioxidant activity and ascorbic acid value in the extraction were proved by the Taguchi method as 20°C/15min. When the colour values were examined, it was determined that the temperature had a significant effect on the colour values obtained in broccoli extract. [ABSTRACT FROM AUTHOR]

[Display omitted] • The bioaccessibility of GABA in the intestinal digestion (GID) reaches 59% in lupin sprouts. • Phenolics and flavonoids showed bioaccessibility reaching 82.56 and 114.20% post-intestinal digestion. • Certain isoflavones, exclusively identified in 7-day sprouts, displayed resilience to GID. • Germinated samples exhibited superior antioxidant activity compared to ungerminated ones after GID. • Germination enhances lupinus' ability to combat oxidative stress. Germination is a process that enhances the content of health-promoting secondary metabolites. However, the bioaccessibility of these compounds depends on their stability and solubility throughout the gastrointestinal tract. The study aimed to explore how germination time influences the content and bioaccessibility of γ-aminobutyric acid and polyphenols and antioxidant capacity of lupin (Lupinus angustifolius L.) sprouts during simulated gastrointestinal digestion. Gamma-aminobutyric acid showed a decrease following gastrointestinal digestion (GID) whereas phenolic acids and flavonoids exhibited bioaccessibilities of up to 82.56 and 114.20%, respectively. Although the digestion process affected the profile of phenolic acids and flavonoids, certain isoflavonoids identified in 7-day sprouts (G7) showed resistance to GID. Germination not only favored antioxidant activity but also resulted in germinated samples exhibiting greater antioxidant properties than ungerminated counter parts after GID. Intestinal digests from G7 did not show cytotoxicity in RAW 264.7 macrophages, and notably, they showed an outstanding ability to inhibit the production of reactive oxygen species. This suggests potential benefit in mitigating oxidative stress. These findings contribute to understand the dynamic interplay between bioprocessing and digestion in modulating the bioaccessibility of bioactive compounds in lupin, thereby impacting health. [ABSTRACT FROM AUTHOR]