13 results on '"Khan, Shah Alam"'
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2. Green synthesis of silver nanoparticles using Omani pomegranate peel extract and two polyphenolic natural products: characterization and comparison of their antioxidant, antibacterial, and cytotoxic activities
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Saad, Pasent Gharib, Castelino, Rohan Daniel, Ravi, Vimal, Al-Amri, Issa Sulaiman, and Khan, Shah Alam
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- 2021
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3. In silico studies on olive oil polyphenolic natural products to identify neuroprotective lead compounds beneficial in the treatment of Alzheimer's disease
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Hachani, Khouloud, Othmani, Fahd, Essam, Mohamed, Akhtar, Md Jawaid, and KHAN, SHAH ALAM
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Alzheimer’s disease ,Neuroprotection ,Phenolic compounds ,Antioxidant ,AChE ,BACE-1 - Abstract
Alzheimer’s disease (AD) is one of the most common neurodegenerative disorders. Nearly 44 million people across the globe are living with it. In spite of tremendous progress in understanding the pathophysiology of AD, only a few drugs have been approved by FDA to date that too provides only symptomatic relief. We have contemplated historical and religious backgrounds in addition to the literature review and concluded that polyphenolic natural products from olive oil can be used for the treatment of AD. The current computational study was designed to investigate the potential of phenolic metabolites present in olive oil to identify lead molecule(s) that could help in fighting against AD. A total of 21 phenolic compounds from olive oil were selected, and their SMILES notations were generated using Chemsketch. Cheminformatics software such as, Molinspiration to predict the bioactivity scores and physicochemical properties, PASS to predict the acetylcholinesterase (AChE) inhibition, neuroprotective, antioxidants, and anti-inflammatory activities; OSIRIS for pharmacokinetic profile and toxicity and Autodock Vina for molecular docking were used for in silico studies. The results were compared with four clinically used AD drugs. All the tested compounds were predicted to possess anti-inflammatory activity (0.357-0.831 Pa score) and antioxidant activity (0.320-0.903 Pa values), but none of the compounds was found to be a butyrylcholinesterase inhibitor. Out of 21 initial polyphenolic compounds, we selected the best two bioactive compounds, luteolin and elenolic acid, based on their bioactivity and toxicity profile. Luteolin showed the most stable binding to both beta-secretase- 1 (BACE) and AChE enzymes followed by elenolic acid. It is concluded that luteolin and elenolic acid are the most potent polyphenolic compounds of the olive, which act at multiple targets in AD pathogenesis. These compounds hold promise for the development of anti-Alzheimer's therapy., Arabian Journal of Medicinal and Aromatic Plants, Vol 9, No 1 (2023)
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- 2023
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4. Gas chromatography-mass spectrometry analysis and in vitro biological studies on fixed oil isolated from the waste pits of two varieties of Olea europaea L.
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Abdelrahman Muna Hayder, Hussain Reem Osama, Shaheed Dhulfiqar Sami, AbuKhader Majed, and Khan Shah Alam
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antioxidant ,antimicrobial ,cytotoxic activity ,olive oil ,olive seeds ,Oils, fats, and waxes ,TP670-699 - Abstract
Olive oil isolated from the fruits of the Olea europaea L. is an important part of Mediterranean diet. It is known for its diverse biological actions. Furthermore, a little amount of fixed oil and other bioactive components can also be extracted from the olive seeds which are considered as byproduct of olive oil extraction. Therefore, this study was designed to analyze the fatty acid composition and to perform in vitro biological studies on fixed oil isolated from olive seeds. The fixed oil was isolated from the olive seeds of Syrian and Greek black olive fruits by using Soxhlet apparatus. The purity was checked by measuring its refractive index. Composition of two isolated oils and a commercially available virgin olive oil was determined by preparing their Methyl esters (FAME) followed by GC-MS analysis. Various in vitro assay methods were used to investigate activities such as antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH), cytotoxic activity by using Brine shrimps lethality bioassay and antimicrobial activity against two bacterial strains; Staphylococcus aureus and Escherichia coli by agar well diffusion method. GC-MS analysis revealed that the two isolated oils differ quantitatively in chemical composition with oleic acid identified as the major chemical constituent (62.6% and 73.56%). Both the fixed seed oils showed a concentration dependent DPPH radical scavenging activity ranging from 8 to 76% inhibition. The oils also exhibited excellent cytotoxic activity but no antimicrobial activity was observed. The chemical composition of the isolated fixed olive seed oil is found to be almost similar to the commercially available fruit olive oil. The fixed oil from the seeds of olive fruits possesses useful biological actions. Further studies are needed to isolate and quantify their bioactive constituents.
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- 2019
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5. Chemical composition, in vitro antibacterial and antioxidant potential of Omani Thyme essential oil along with in silico studies of its major constituent.
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Alsaraf, Shahad, Hadi, Zainab, Al-Lawati, Wafa Mustafa, Al Lawati, Amal Abdullah, and Khan, Shah Alam
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• Chemical profiling of Omani Thymus vulgaris species was done by GC-MS analysis. • The GC-MS analysis of thyme essential oil established it to be a carvacrol chemotype. • Thyme essential oil exhibited better antimicrobial spectrum than ampicillin. • Thyme essential oil showed significant in vitro free radical scavenging activity in DPPH assay method. • In silico PASS prediction studies revealed carvacrol to possess useful bioactivities including cytotoxicity. Thyme is an evergreen shrub that has been utilized in traditional medicine and culinary packages for centuries because of its aroma and medicinal properties. The medicinal properties of thyme leaves are attributed to its volatile constituents. The study aimed to identify the volatile constituents in the oil extracted from the Thyme sp grown in Oman and to evaluate its in vitro antibacterial and antioxidant activity. Fresh Thyme leaves were collected from Muscat, Sultanate of Oman in the month of September 2018. Thyme oil was isolated from leaves by hydro-distillation. The volatile chemical constituents present in the thyme oil were analyzed by Gas Chromatograph coupled with Mass Spectrometer. The ability of isolated oil to scavenge free radicals was evaluated by an in vitro DPPH assay method while antimicrobial activity was tested against S. aureus and E. coli bacterial strains by disc diffusion method. The bioactivity of the major constituent of the oil was predicted with the help of PASS and CLC-pred software. Molecular docking studies were performed by docking server. GC–MS analysis of thyme oil revealed the presence of 11 components. Carvacrol and γ-terpinene were identified as the major volatile constituents in thyme oil. Thyme oil inhibited 71.57% of DPPH radicals at 40 µg/mL concentration. Thyme oil displayed the better antimicrobial activity than the standard drug ampicillin against both the bacteria. Antiseptic, antiinfective, reductant and antimutagenic bioactivities of carvacrol as predicted by PASS support the in vitro experimental results. CLC-pred showed carvacrol to be active against Metastatic melanoma, Oligodendroglioma and Non small cell lung cancer cell lines. The antioxidant and antimicrobial activity of Omani thyme oil could be due to its high content of carvacrol. Omani thyme oil seems to be a viable alternative source of natural antimicrobial agent(s) and warrants further studies to ascertain its therapeutic spectrum of biological studies. [ABSTRACT FROM AUTHOR]
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- 2020
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6. In vitro inhibitory effects on α-glucosidase and α-amylase level and antioxidant potential of seeds of Phoenix dactylifera L.
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Khan, Shah Alam, Al Kiyumi, Amira Rashid, Al Sheidi, Manal Saif, Al Khusaibi, Tagreed Salim, Al Shehhi, Noura Mohammed, and Alam, Tanveer
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GLUCOSIDASE inhibitors ,AMYLASE inhibitors ,HYPOGLYCEMIC agents ,HYDROGEN peroxide ,DIABETES prevention - Abstract
Objective To evaluate and compare the antioxidant activity, total phenolic contents (TPCs) and in vitro antidiabetic activity of various pits extracts obtained from five Omani date cultivars. Methods Sun-dried mature fruits of five Omani date varieties, namely, Fardh, Naghal, Khalas, Khinazi and Khasab were purchased from the local market in Muscat, Oman in the month of September 2014. Four seed extracts viz. water, ethanol, methanol and acetone were prepared for each date variety and their antioxidant activities were investigated by 1,1-diphenyl-2-picrylhydrazyl, hydrogen peroxide scavenging method and reducing power assay method, respectively. In vitro antidiabetic activity of the date pit extracts was evaluated by measuring their inhibitory effect on α-glucosidase and α-amylase level. TPCs were also quantified colorimetrically. Results The results indicated that TPC of date seeds was solvent dependent. Acetone, ethanol and methanol were found to be significantly better solvents than water in extracting phenolic compounds from the date seeds. Pit extracts exhibited moderate to good in vitro antioxidant activity and increased reducing power. Among all date pit extracts, water extract exhibited significant in vitro antidiabetic activity in comparison to standard drug, acarbose. Conclusions The present study confirms that disposed waste of Omani dates is a rich source of dietary antioxidant because of its high TPC. The pits due to their inhibitory effects on α-glucosidase and α-amylase level could be used as a monotherapy along with an appropriate diabetic diet and exercise or might be in conjunction with antidiabetic therapy to manage and prevent progression of diabetes. [ABSTRACT FROM AUTHOR]
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- 2016
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7. Estimation of total phenolic content, in–vitro antioxidant and anti–inflammatory activity of flowers of Moringa oleifera.
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Alhakmani, Fatma, Kumar, Sokindra, and Khan, Shah Alam
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PHENOLS ,ANTIOXIDANTS ,ANTI-inflammatory agents ,MORINGA oleifera ,COMPARATIVE studies ,ETHANOL ,IN vitro studies ,THERAPEUTICS - Abstract
Abstract: Objective: To evaluate and compare the antioxidant potential and anti–inflammatory activity of ethanolic extract of flowers of Moringa oleifera (M. oleifera) grown in Oman. Methods: Flowers of M. oleifera were collected in the month of December 2012 and identified by a botanist. Alcoholic extract of the dry pulverized flowers of M. oleifera were obtained by cold maceration method. The ethanolic flower extract was subjected to preliminary phytochemical screening as the reported methods. Folin–Ciocalteu reagent was used to estimate total phenolic content. DPPH was used to determine in–vitro antioxidant activity and anti–inflammatory activity of flowers was investigated by protein denaturation method. Results: Phytochemical analysis of extract showed presence of major classes of phytochemicals such as tannins, alkaloids, flavonoids, cardiac glycosides etc. M. oleifera flowers were found to contain 19.31 mg/g of gallic acid equivalent of total phenolics in dry extract but exhibited moderate antioxidant activity. The anti–inflammatory activity of plant extract was significant and comparable with the standard drug diclofenac sodium. Conclusions: The results of our study suggest that flowers of M. oleifera possess potent anti–inflammatory activity and are also a good source of natural antioxidants. Further study is needed to identify the chemical compounds responsible for their anti–inflammatory activity. [Copyright &y& Elsevier]
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- 2013
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8. A review on therapeutic potential of Nigella sativa: A miracle herb.
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Ahmad, Aftab, Husain, Asif, Mujeeb, Mohd, Khan, Shah Alam, Najmi, Abul Kalam, Siddique, Nasir Ali, Damanhouri, Zoheir A., and Anwar, Firoz
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BLACK cumin ,HERBAL medicine ,BRONCHODILATOR agents ,ESSENTIAL oils ,MEDICINAL plants ,RANUNCULACEAE ,ANTIOXIDANTS - Abstract
Abstract: Nigella sativa (N. sativa) (Family Ranunculaceae) is a widely used medicinal plant throughout the world. It is very popular in various traditional systems of medicine like Unani and Tibb, Ayurveda and Siddha. Seeds and oil have a long history of folklore usage in various systems of medicines and food. The seeds of N. sativa have been widely used in the treatment of different diseases and ailments. In Islamic literature, it is considered as one of the greatest forms of healing medicine. It has been recommended for using on regular basis in Tibb-e-Nabwi (Prophetic Medicine). It has been widely used as antihypertensive, liver tonics, diuretics, digestive, anti-diarrheal, appetite stimulant, analgesics, anti-bacterial and in skin disorders. Extensive studies on N. sativa have been carried out by various researchers and a wide spectrum of its pharmacological actions have been explored which may include antidiabetic, anticancer, immunomodulator, analgesic, antimicrobial, anti-inflammatory, spasmolytic, bronchodilator, hepato-protective, renal protective, gastro-protective, antioxidant properties, etc. Due to its miraculous power of healing, N. sativa has got the place among the top ranked evidence based herbal medicines. This is also revealed that most of the therapeutic properties of this plant are due to the presence of thymoquinone which is major bioactive component of the essential oil. The present review is an effort to provide a detailed survey of the literature on scientific researches of pharmacognostical characteristics, chemical composition and pharmacological activities of the seeds of this plant. [Copyright &y& Elsevier]
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- 2013
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9. Anti-Alzheimer potential of a waste by-product (peel) of Omani pomegranate fruits: Quantification of phenolic compounds, in-vitro antioxidant, anti-cholinesterase and in-silico studies.
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Khokar, Rehab, Hachani, Khouloud, Hasan, Mariya, Othmani, Fahd, Essam, Mohammad, Al Mamari, Aliya, UM, Dhanalekshmi, and Khan, Shah Alam
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FRUIT skins ,PHYTOCHEMICALS ,PHENOLS ,POMEGRANATE ,MOLECULAR docking ,FRUIT ,DISEASE progression - Abstract
Alzheimer's disease (AD) is an irreversible progressive neurodegenerative disease. Punica granatum L., (P. granatum) is one of the most important commercial crops of Oman with traditional medicinal claims. The study aimed to quantify the phenolic content of the Omani pomegranate peel extracts and to investigate their antioxidant, anti-cholinesterase activities along with computational studies for the development of AD therapy. The dried peels of P. granatum fruits were exhaustively extracted with chloroform, ethyl acetate and butanol. Peel extracts were subjected to the preliminary phytochemical screening. Colorimetric methods were used to quantify total phenolic and flavonoids contents. The neuroprotective potential of the peel extracts was evaluated by assessing the in-vitro antioxidant activity and their ability to inhibit acetylcholinesterase (AChE) enzyme activity. In-silico analysis and molecular docking of the most promising phenolic metabolite present in pomegranate peel was also carried out. The phytochemical screening showed the presence of flavonoids, tannins, alkaloids, carbohydrates and terpenoids. The total phenolic, flavonoids content & antioxidant activity was found to be higher in the butanol extract. Surprisingly, butanol extract showed slightly lower AChE inhibitory activity than chloroform extract. Catechin, a polyphenolic compound present in the peel showed lower binding energy for AChE in comparison to Beta-secretase-1 (BACE-1). It formed three and two hydrogen bonds with AChE and BACE-1, respectively. The results of the current study provided evidence that butanol extract is rich in phenolics and possesses excellent antioxidant activity. Furthermore, in silico and in-vitro studies conducted on P. granatum revealed promising anti-AD activity. Further investigations must be carried out to isolate and develop the chemical constituents of pomegranate peel as neuroprotective agent(s). [Display omitted] • Phytochemical screening and quantification of phenolic compounds in an agro-waste. • Evaluation of antioxidant and anticholinesterase activity of pomegranate peel extracts. • In silico studies and molecular docking studies on the polyphenolic compounds found in pomegranate peel. • Butanol solvent was found to be optimum for the isolation of polyphenolic compounds. • Pomegranate peel could be used as an alternative source of natural antioxidants. [ABSTRACT FROM AUTHOR]
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- 2021
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10. Chemical profiling, cytotoxic and antioxidant activity of volatile oil isolated from the mint (Mentha spicata L.,) grown in Oman.
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Alsaraf, Shahad, Hadi, Zainab, Akhtar, Md Jawaid, and Khan, Shah Alam
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SPEARMINT ,ESSENTIAL oils ,OIL paint ,THYROID gland ,ANTIOXIDANTS ,LYMPHOBLASTIC leukemia - Abstract
The study aimed to characterize the volatile constituents of Mentha spicata L., grown in Oman, and to investigate in vitro cytotoxic and antioxidant activities of the volatile oil. The pale-yellow color essential oil (% yield 0.55% v/w) showed the presence of thirteen compounds representing 99.19% of the total constituents. d-Carvone (65.21%) and dl -limonene (27.28%) were identified as the major volatile chemical constituents. Mint oil exhibited promising antioxidant (IC 50 = 26.64 μg/mL) and cytotoxic activity (LC 50 = 245 μg/mL). CLC predicted d-carvone to be active against thyroid gland undifferentiated (anaplastic) carcinoma, pancreatic carcinoma, brain carcinoma, and lymphoblastic leukemia while dl -limonene could be effective against skin melanoma, breast adenocarcinoma in addition to the above carcinomas. Omani spearmint volatile oil exhibited promising cytotoxic and antioxidant activities. However, further detailed studies are warranted to develop mint oil as a natural antioxidant and to unlock its hidden cytotoxic potential. [Display omitted] • Chemical profiling of Omani Mentha spicata species was done by GC-MS analysis. • The GC-MS analysis of mentha volatile oil established it to be a carvone chemotype. • Mentha essential oil exhibited promising antioxidant and cytotoxic activities. • Computational studies revealed carvone and limonene to possess useful bioactivities including cytotoxicity. [ABSTRACT FROM AUTHOR]
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- 2021
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11. Quantification of bioactive components and evaluation of antioxidative potential of different floral origin honey from arid regions of Oman.
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Al Kiyumi, Eman H., Al Rashdi, Balqees S., Al Alawi, Aya R., Al Balushi, Asma A., Al Hooti, Shatha N., Al Hosni, Sumaiya I., Dhanalekshmi, U.M., and Khan, Shah Alam
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BIOACTIVE compounds ,ARID regions ,HONEY ,NUTRITION ,FLAVONOIDS ,WATER acidification ,PYRROLIZIDINES - Abstract
This preliminary study was undertaken to evaluate and compare the physicochemical properties, total phenolic content, total flavonoid content and in vitro antioxidant activity of 22 types of monofloral and heterofloral Omani honey. Total phenolic and flavonoid contents were quantified colorimetrically while antioxidant activity was evaluated by 1,1 diphenyl picrylhydrazyl (DPPH) free radical scavenging assay method. Our result clearly shows significant variations among honey samples for all evaluated parameters. Monofloral honey from Salalah, Samail, and Al-Khaboora showed good inhibition of DPPH radicals. The highest amount of total phenol was found in monofloral honey from Al-Dahirah while the highest amount of flavonoid was observed in a monofloral variety from Salalah. A direct relationship between phenol, flavonoid and inhibition of DPPH was also observed. It can be concluded that the monofloral types of honey can be considered particularly useful for human nutrition because of their high phenolic and flavonoid contents which are known for their antioxidant activity. [Display omitted] • Quantification of total phenol and flavonoids of 22 honey samples collected from different regions of Oman. • Free radical scavenging activity of honey samples is reported to predict the nutritional value of honey. • Antibacterial activity of four honey samples is reported. • A comparison of honey samples with respect to refractive index, Brix%, acidity and % of water. [ABSTRACT FROM AUTHOR]
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- 2021
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12. Determination of total phenol, in-vitro antioxidant and anti-inflammatory activity of seeds and fruits of Zizyphus spina-christi grown in Oman.
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Alhakmani, Fatma, Khan, Shah Alam, and Ahmad, Aftab
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ZIZIPHUS ,PHENOL ,PHENOLS ,ANTIOXIDANTS - Abstract
Objective To perform phytochemical screening and to evaluate the in-vitro antioxidant and anti-inflammatory activities of ethanolic extract of seeds and fruits of Zizyphus spina-christi (ZSC) growing in Oman. Methods Alcoholic extract of the dry powdered seeds and fruits of ZSC was obtained by cold maceration method and was subjected to preliminary phytochemical screening. Total phenolic content were estimated by using Folin-Ciocalteu reagent and 1,1-diphenyl-2-picrylhydrazyl (DPPH) was used to determine in-vitro antioxidant activity of plant extracts. Anti-inflammatory activity was investigated by protein denaturation method. Results Phytochemical analysis of both the extracts revealed the presence of major classes of phytochemicals such as tannins, alkaloids, flavonoids, cardiac glycosides etc.. ZSC seeds were found to contain the highest total phenolics but ZSC fruits exhibited the maximum antioxidant activity. The anti-inflammatory activity of both parts of the plant extract was significant and comparable with the standard anti-inflammatory drug, diclofenac. Conclusions Based on the results of this pilot study, it can be concluded that ZSC is a good source of natural antioxidants which can be used to prevent progression of many chronic diseases. Further detailed phytochemical studies are needed to identify the chemical compounds responsible for exhibiting potent anti-inflammatory activity. [ABSTRACT FROM AUTHOR]
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- 2014
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13. Coumarin linked heterocyclic hybrids: A promising approach to develop multi target drugs for Alzheimer's disease.
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Husain, Asif, Balushi K, Al, Akhtar, Md Jawaid, and Khan, Shah Alam
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TACRINE , *ALZHEIMER'S disease , *STRUCTURE-activity relationships , *BRAIN degeneration , *DRUG design , *COUMARIN derivatives - Abstract
• Hybridization drug design strategies with rationale to obtain multi target directed ligands (MTDLs) as anti-Alzheimer drugs. • The review covers SAR studies for further development of coumarin-hybrids as therapeutic agents for the Alzheimer's disease (AD). • It provides an overview of research work carried out on di and tri-hybridized coumarin conjugates during 2010–2020. • Chemical structure and biochemical targets of naturally occurring coumarin derivatives with anti-AD properties. • Patents granted to coumarin derivatives for AD treatment. Alzheimer's disease (AD) is a major progressive and perplexing cortical degenerative disease of the brain in the elderly. It contributes to a significant socioeconomic burden on patients, families, and care providers in comparison to cancer, cardiovascular diseases, and stroke in general. Recently, a hybridized approach based on multi-target directed ligands (MTDLs) has received considerable attention to develop therapeutic agents for AD which act at multiple targets. Approximately 20% of the drugs approved in the past couple of years were developed using the same strategy and thus it seems to be the right approach to develop AD therapy. The comprehensive and updated review on coumarin hybrids as MTDLs in AD therapy is compiled using several electronic databases such as Scopus, Google Scholar, SciFinder, Mendeley, and PubMed. The research studies published on coumarin hybrids in the last decade (2010- 2020) have been covered in this review article. This updated review highlights the importance of coupling (hybridization) coumarin scaffold with other bioactive pharmacophores to develop MTDLs for AD therapy. It also covers the patents granted to coumarin derivatives for AD treatment, natural products containing coumarin scaffold with anti-Alzheimer spectrum, the rationale for coupling pharmacophore fragments, detailed structure activity relationship (SAR), and molecular docking studies. The most potent and promising hybrid compounds have also been identified for further development of AD drugs. Authors are of the opinion that hybridization strategy would soon yield drug candidates for the treatment of AD. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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