1. Phospholipid-chitosan hybrid nanoliposomes promoting cell entry for drug delivery against cervical cancer.
- Author
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Saesoo S, Bunthot S, Sajomsang W, Gonil P, Phunpee S, Songkhum P, Laohhasurayotin K, Wutikhun T, Yata T, Ruktanonchai UR, and Saengkrit N
- Subjects
- Antineoplastic Agents pharmacology, Apoptosis drug effects, Cell Proliferation drug effects, Cisplatin pharmacology, Dose-Response Relationship, Drug, Drug Screening Assays, Antitumor, Female, Humans, Liposomes chemistry, Molecular Structure, Structure-Activity Relationship, Uterine Cervical Neoplasms pathology, Antineoplastic Agents administration & dosage, Chitosan chemistry, Cisplatin administration & dosage, Drug Delivery Systems, Nanostructures chemistry, Phospholipids chemistry, Uterine Cervical Neoplasms drug therapy
- Abstract
This study emphasizes the development of a novel surface modified liposome as an anticancer drug nanocarrier. Quaternized N,O-oleoyl chitosan (QCS) was synthesized and incorporated into liposome vesicles, generating QCS-liposomes (Lip-QCS). The Lip-QCS liposomes were spherical in shape (average size diameter 171.5±0.8nm), with a narrow size distribution (PDI 0.1±0.0) and zeta potential of 11.7±0.7mV. In vitro mucoadhesive tests indicated that Lip-QCS possesses a mucoadhesive property. Moreover, the presence of QCS was able to induce the cationic charge on the surface of liposome. Cellular internalization of Lip-QCS was monitored over time, with the results revealing that the cell entry level of Lip-QCS was elevated at 24h. Following this, Lip-QCS were then employed to load cisplatin, a common platinum-containing anti-cancer drug, with a loading efficiency of 27.45±0.78% being obtained. The therapeutic potency of the loaded Lip-QCS was investigated using a 3D spheroid cervical cancer model (SiHa) which highlighted their cytotoxicity and apoptosis effect, and suitability as a controllable system for sustained drug release. This approach has the potential to assist in development of an effective drug delivery system against cervical cancer., (Copyright © 2016 Elsevier Inc. All rights reserved.)
- Published
- 2016
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