1. Bioassay-guided isolation and identification of cytotoxic compounds from Gymnosperma glutinosum leaves.
- Author
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Quintanilla-Licea R, Morado-Castillo R, Gomez-Flores R, Laatsch H, Verde-Star MJ, Hernández-Martínez H, Tamez-Guerra P, Tamez-Guerra R, and Rodríguez-Padilla C
- Subjects
- Animals, Antineoplastic Agents chemistry, Cell Line, Tumor, Diterpenes chemistry, Drug Discovery, Drug Screening Assays, Antitumor, Hydrocarbons chemistry, Hydrocarbons isolation & purification, Hydrocarbons pharmacology, Lymphoma drug therapy, Mice, Plant Extracts chemistry, Plant Extracts pharmacology, Antineoplastic Agents isolation & purification, Antineoplastic Agents pharmacology, Cycadopsida chemistry, Diterpenes isolation & purification, Diterpenes pharmacology, Plant Leaves chemistry
- Abstract
Bioassay-guided fractionation of hexane extracts of Gymnosperma glutinosum (Asteraceae) leaves, collected in North Mexico, afforded the known compounds hentriacontane (1) and (+)-13S,14R,15-trihydroxy-ent-labd-7-ene (2), as well as the new ent-labdane diterpene (-)-13S,14R,15-trihydroxy-7-oxo-ent-labd-8(9)-ene (3). In addition, D-glycero-D-galactoheptitol (4) was isolated from the methanolic extract of this plant. Their structures were established on the basis of high-field 1D- and 2D NMR methods supported by HR-MS data. The cytotoxic activity was determined by using the in vitro L5178Y-R lymphoma murine model. Hentriacontane (1) and the new ent-labdane 3 showed weak cytotoxicity, whereas the ent-labdane 2 showed significant (p < 0.05) and concentration dependent cytotoxicity (up to 78%) against L5178Y-R cells at concentrations ranging from 7.8 to 250 μg/mL.
- Published
- 2012
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