1. Four sulfur-containing compounds with anti-colon cancer effect from marine-derived fungus Aspergillus terreus.
- Author
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Zhang GY, Liu BJ, Pan HL, Li HJ, Huang ZF, Mahmud T, Ma WZ, and Lan WJ
- Subjects
- Humans, Molecular Structure, HCT116 Cells, Caco-2 Cells, Colonic Neoplasms drug therapy, Aspergillus chemistry, Sulfur Compounds pharmacology, Sulfur Compounds isolation & purification, Antineoplastic Agents pharmacology, Antineoplastic Agents isolation & purification
- Abstract
Sulfur-containing natural products possess a variety of biological functions including antitumor, antibacterial, anti-inflammatory and antiviral activities. In this study, four previously undescribed sulfur-containing compounds asperteretals L and M, terreins A and B, together with 17 known compounds were obtained from a culture of marine fungus A. terreus supplemented with inorganic sulfur source Na
2 SO4 . Their planar structures and absolute configurations were elucidated by NMR, HRESIMS, and ECD experiments. The in vitro cytotoxicities of compounds 1-21 against HCT-116 and Caco-2 were evaluated by SRB assay. Asperteretal M (2) exhibited activity against HCT-116 with the IC50 value at 30μM. The antiproliferative effect of asperteretal M was confirmed by colony formation assay and cell death staining. Furthermore, the preliminary study on the anti-colon cancer mechanism of asperteretal M was performed by RNA-seq analysis. Western blotting validated that asperteretal M significantly decreased the expression of cell-cycle regulatory proteins CDK1, CDK4, and PCNA in a concentration-dependent manner., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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