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Background: Salmonella infections represent a major global public health concern due to their widespread zoonotic transmission, antimicrobial resistance, and associated morbidity and mortality. This review aimed to summarize the zoonotic nature of Salmonella, the challenges posed by antimicrobial resistance, the global burden of infections, and the need for effective vaccination strategies to mitigate the rising threat of Salmonella. Methods: A systematic review of literature was conducted using databases such as PubMed, Scopus, Web of Science, and Google Scholar. Relevant studies published in English were identified using keywords including Salmonella, vaccination, antimicrobial resistance, and public health. Articles focusing on epidemiology, vaccine development, and strategies to control Salmonella infections were included, while conference abstracts and non-peer-reviewed studies were excluded. Results: Salmonella infections result in approximately 95 million global cases annually, with an estimated 150,000 deaths. Regional variations were evident, with higher infection rates in low- and middle-income countries due to poor sanitation and food safety standards. Salmonella Enteritidis and S. Typhimurium were the most prevalent serovars associated with human infections. The review highlighted an alarming rise in multidrug-resistant (MDR) Salmonella strains, particularly due to the overuse of antibiotics in humans and livestock. Despite progress in vaccine development, challenges remain in achieving a universal vaccine that targets diverse Salmonella serovars. Live-attenuated, killed, recombinant, subunit, and conjugate vaccines are currently under development, but limitations such as efficacy, cost, and accessibility persist. Conclusions: Salmonella infections continue to impose a significant burden on global health, exacerbated by rising antimicrobial resistance. There is an urgent need for a multifaceted approach, including improved sanitation, prudent antibiotic use, and the development of affordable, broad-spectrum vaccines. Strengthening surveillance systems and promoting collaborative global efforts are essential to effectively control and reduce the burden of Salmonella. [ABSTRACT FROM AUTHOR]

Background: Africa has one of the highest incidences of gonorrhea. Neisseria gonorrhoeae is gaining resistance to most of the available antibiotics, compromising treatment across the world. Whole-genome sequencing (WGS) is an efficient way of predicting AMR determinants and their spread in the population. Recent advances in next-generation sequencing technologies like Oxford Nanopore Technology (ONT) have helped in the generation of longer reads of DNA in a shorter duration with lower cost. Increasing accuracy of base-calling algorithms, high throughput, error-correction strategies, and ease of using the mobile sequencer MinION in remote areas lead to its adoption for routine microbial genome sequencing. To investigate whether MinION-only sequencing is sufficient for WGS and downstream analysis in resource-limited settings, we sequenced the genomes of 14 suspected N. gonorrhoeae isolates from Nairobi, Kenya. Methods: Using WGS, the isolates were confirmed to be cases of N. gonorrhoeae (n = 9), and there were three co-occurrences of N. gonorrhoeae with Moraxella osloensis and N. meningitidis (n = 2). N. meningitidis has been implicated in sexually transmitted infections in recent years. The near-complete N. gonorrhoeae genomes (n = 10) were analyzed further for mutations/factors causing AMR using an in-house database of mutations curated from the literature. Results: We observe that ciprofloxacin resistance is associated with multiple mutations in both gyrA and parC. Mutations conferring tetracycline (rpsJ) and sulfonamide (folP) resistance and plasmids encoding beta-lactamase were seen in all the strains, and tet(M) -containing plasmids were identified in nine strains. Phylogenetic analysis clustered the 10 isolates into clades containing previously sequenced genomes from Kenya and countries across the world. Based on homology modeling of AMR targets, we see that the mutations in GyrA and ParC disrupt the hydrogen bonding with quinolone drugs and mutations in FolP may affect interaction with the antibiotic. Conclusion: Here, we demonstrate the utility of mobile DNA sequencing technology in producing a consensus genome for sequence typing and detection of genetic determinants of AMR. The workflow followed in the study, including AMR mutation dataset creation and the genome identification, assembly, and analysis, can be used for any clinical isolate. Further studies are required to determine the utility of real-time sequencing in outbreak investigations, diagnosis, and management of infections, especially in resource-limited settings. [ABSTRACT FROM AUTHOR]

• This study explores the potential of combined phytoremediation using Andrographolide and Ajwain. • Selected combinations mitigated signs of infection. • Offers an innovative and sustainable solution to address antimicrobial resistance in chicken. The emergence of antibiotic resistance in pathogenic bacteria, including Salmonella gallinarum , poses a significant challenge to poultry health and food safety. In response, alternative strategies are urgently needed to mitigate bacterial infections without exacerbating antibiotic resistance. Phytoremediation, a sustainable and environmentally friendly approach, harnesses the natural detoxification capabilities of plants to remediate contaminants. This study explores the potential of combined phytoremediation using Andrographolide, derived from Andrographis paniculata , and Ajwain derived from Trachyspermum ammi as promising alternatives to antibiotics for treating Salmonella gallinarum infection in poultry. Andrographolide, known for its potent antimicrobial properties, exhibits inhibitory effects while Ajwain, rich in bioactive compounds, possesses antimicrobial and immunomodulatory properties. By leveraging their combined phytoremediation potential, Andrographolide and Ajwain offer a multifaceted approach to combat Salmonella gallinarum within the poultry environment. The study employed a rigorous experimental design, including in vitro assessments of antimicrobial susceptibility, cytotoxicity, and optimal concentration determination. Following this, in vivo experiments were conducted using a chicken model infected with Salmonella gallinarum. Results demonstrated that the selected combinations effectively reduced mortality rates, alleviated clinical symptoms, and mitigated gross pathological signs associated with Salmonella infection. Gene expression studies indicated a downregulation of proinflammatory cytokines, underscoring potential implications of a combined phytoremediation strategy as an innovative and sustainable solution to address Salmonella gallinarum infections in poultry production systems. [ABSTRACT FROM AUTHOR]