1. Platelet-released growth factors induce psoriasin in keratinocytes: Implications for the cutaneous barrier.
- Author
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Bayer, Andreas, Lammel, Justus, Lippross, Sebastian, Klüter, Tim, Behrendt, Peter, Tohidnezhad, Mersedeh, Pufe, Thomas, Cremer, Jochen, Jahr, Holger, Rademacher, Franziska, Gläser, Regine, and Harder, Jürgen
- Subjects
WOUND healing ,KERATINOCYTES ,DISEASE progression ,BLOOD platelets ,DISEASE complications - Abstract
Millions of patients around the world suffer minor or major extremity amputation due to progressive wound healing complications of chronic or infected wounds, the therapy of which remains a challenge. One emerging therapeutic option for the treatment of these complicated wounds is the local application of an autologous thrombocytes concentrate lysate ( e.g . platelet-released growth factors ((PRGF)) or Vivostat PRF ® ) that contains a multitude of chemokines, cytokines and growth factors and is therefore supposed to stimulate the complex wound healing process. Although PRGF and Vivostat PRF ® are already used successfully to support healing of chronic, hard-to-heal and infected wounds the underlying molecular mechanisms are not well understood. Psoriasin, also termed S100A7, is a multifunctional antimicrobial protein expressed in keratinocytes and is involved in various processes such as wound-healing, angiogenesis, innate immunity and immune-modulation. In this study, we investigated the influence of PRGF on psoriasin expression in human primary keratinocytes in vitro and the influence of Vivostat PRF ® on psoriasin expression in experimentally generated skin wounds in vivo . PRGF treatment of primary keratinocytes caused a significant concentration- and time-dependent increase of psoriasin gene and protein expression in vitro that were partially mediated by the epidermal growth factor receptor (EGFR) and the interleukin-6 receptor (IL-6R). In accordance with these cell culture data, Vivostat PRF ® induced a significant psoriasin gene and protein expression when applied to artificially generated skin wounds in vivo . The observed psoriasin induction in keratinocytes may contribute to the wound healing-promoting effects of therapeutically used thrombocyte concentrate lysates. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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