1. In-vitro Assays for Antimicrobial Assessment
- Author
-
Jaya Vikas Kurhekar, Maria Catherine B. Otero, and Genevieve D. Tupas
- Subjects
Minimum inhibitory concentration ,Chromatography ,Minimum bactericidal concentration ,Chemistry ,Antimicrobial assay ,In vitro toxicology ,Biofilm ,Bacterial growth ,Antimicrobial ,Diffusion methods - Abstract
In vitro antimicrobial assessment of natural extracts or pure compounds involves the use of assays to evaluate their potential to kill or inhibit microbial growth in a laboratory setting. Several methods have been developed for this purpose, which include Agar Well Diffusion and Disc Diffusion methods, Dynamic Contact assay, Thin-Layer Chromatography-Bioautography, Time-Kill assay and Biofilm assays, Microdilution methods and broth assays for Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), Minimum Doubling Time/Growth Curve (MDT), Flow Cytofluorometric Assay and Bioluminescence Assay. This chapter will provide the readers a brief but comprehensive methodology of these in-vitro assays along with their advantages and disadvantages. It will serve as a guide in choosing the most appropriate antimicrobial assay for their research objectives.
- Published
- 2019
- Full Text
- View/download PDF