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2 results on '"Ueda, Jonata M."'

1. Polyphenol Composition by HPLC-DAD-(ESI-)MS/MS and Bioactivities of Extracts from Grape Agri-Food Wastes.

Author

Ueda, Jonata M., Griebler, Karoline Ribeiro, Finimundy, Tiane C., Rodrigues, Daniele B., Veríssimo, Lavínia, Pires, Tânia C. S. P., Gonçalves, João, Fernandes, Isabel P., Pereira, Eliana, Barros, Lillian, Heleno, Sandrina A., and Calhelha, Ricardo C.

Subjects
*GRAPE seeds, *FOOD additives, *PHENOLS, *EXTRACTS, *ANTIOXIDANT testing, *GRAPES
Abstract

Background: Grape agri-food wastes, such as skin, seeds, and other discarded by-products, contain phytochemical compounds that offer potential health benefits. Methods: This study aimed to investigate the polyphenol composition and bioactivities of different extracts obtained from grape marc and seeds, with the goal of exploring their potential for application as natural food additives. Results: Regardless of the extraction method used (dynamic maceration, ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE)), all extracts exhibited relatively high concentrations of phenolic compounds. The chemical characterization of the extracts revealed the presence of specific compounds and chemical groups associated with each extraction methodology. Moreover, the extracts displayed satisfactory antioxidant activities, especially in inhibiting lipoperoxidation as assessed by the TBARS assay. Additionally, the extracts demonstrated effective inhibition against different strains of bacteria and fungi known as food contaminants. Taken together, these findings indicate that those extracts have the potential to be tested as natural antioxidants and preservatives with sustainable origins in food and beverage systems. Among the extraction methods evaluated, traditional maceration and UAE provided extracts with the highest antioxidant and antimicrobial activities. Conclusions: Our results suggest the opportunity to explore grape marc and seeds discarded by the winery industry in Portugal as natural sources of bioactive compounds, which could be employed as functional food ingredients or technological additives. The valorization of grape biowastes offers a promising strategy to reduce waste and harness their potential health benefits. [ABSTRACT FROM AUTHOR]

Published
2023
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2. Promising preserving agents from sage and basil: a case study with yogurts

Author

Ueda, Jonata M., Pedrosa, Mariana C., Fernandes, Filipa A., Rodrigues, Paula, Melgar Castañeda, Bruno, Dias, Maria Inês, Pinela, José, Calhelha, Ricardo C., Ivanov, Marija, Soković, Marina, Heleno, Sandrina A., Carocho, Márcio, Ineu, Rafael P., Ferreira, Isabel C.F.R., Barros, Lillian, and Ueda J.M.

Subjects
Preservative, Health (social science), food.ingredient, 030309 nutrition & dietetics, Functional foods, Plant Science, Natural preservatives, lcsh:Chemical technology, Yogurts, Health Professions (miscellaneous), Microbiology, Article, yogurts, natural preservatives, functional foods, sustainable technologies, green solvents, 03 medical and health sciences, 0404 agricultural biotechnology, food, lcsh:TP1-1185, Food science, Response surface methodology, Sustainable technologies, 0303 health sciences, biology, Chemistry, SAGE, Salvia officinalis, Basilicum, 04 agricultural and veterinary sciences, Antimicrobial, Ocimum, biology.organism_classification, 040401 food science, food.food, Fermentation, Green solvents, Food Science
Abstract

In the present work, sage (Salvia officinalis L.) and basil (Ocimum basilicum L.) were exploited for their preservative purposes, as viable alternatives to artificial ones. The ultrasound-assisted extraction (UAE) of bioactive compounds was pre-optimized using factorial screening analysis, prior to applying response surface methodology (RSM). The obtained extracts were characterized in terms of phenolic compounds by high-performance liquid chromatography coupled to photodiode array detector and mass spectrometer HPLC-DAD-ESI/MS and bioactivities, namely the antioxidant, antimicrobial and cytotoxic potential. In addition, the most promising extracts were incorporated into yogurts, that were further screened for nutritional and physico-chemical properties and microbial load, over a shelf life of 14 days. According to the obtained results, the solvent percentage is the most relevant factor for obtaining rosmarinic acid-rich extract, followed by the extraction time and ultrasonic power. For the antioxidant and antimicrobial activity, sage showed the best result for both analysis and none of the two plant extracts were hepatotoxic. Finally, both extracts did not show changes in the physicochemical and nutritional characteristics of the yogurts and did not interfere with the growth of lactic acid bacteria, an important microorganism during yogurt fermentation. These results highlight the high potential of sage and basil as natural preservatives. Please add: This research was funded by the Portuguese Foundation for Science and Technology (FCT) and ERDF, through the Incentive System to Research and Technological development, within the Portugal2020 Competitiveness and Internationalization Operational Program for the financial support to BIOMA: POCI_01_0247_FEDER_046112; ERDF through the Regional Operational Program North 2020, within the scope of Project GreenHealth: Norte-01-0145-FEDER-000042. FCT: Portugal for financial support through national funds FCT/MCTES to the CIMO (UIDB/00690/2020) and to F.A. Fernandes PhD grant (SFRH/BD/145467/2019). L. Barros, M.I. Dias and R.C. Calhelha also thank the national funding by FCT, P.I., through the institutional scientific employment program-contract for their contracts and M. Carocho (CEECIND/00831/2018), J. Pinela (CEECIND/01011/2018) and S.A. Heleno (CEECIND/03040/2017) to the national funding by FCT, P.I., through the individual scientific employment program-contracts; ERDF, through the Incentive System to Research and Technological development, within the Portugal2020 Competitiveness and Internationalization Operational Program for the financial support to BIOMA: POCI_01_0247_FEDER_046112; ERDF through the Regional Operational Program North 2020, within the scope of Project GreenHealth: Norte-01-0145-FEDER-000042. Serbian Ministry of Education, Science and Technological Development [Contract No. 451-03-68/2020-14/200007] for MI and MS. info:eu-repo/semantics/publishedVersion

Published
2018

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