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In the present work, 2‐hydroxy‐1‐naphthaldhyde and 2‐hydrazino‐N‐phenyl‐2‐thioxo‐acetamide are combined to create a new Schiff base. The novel ligand and its six new binuclear complexes with Ni(II), Cu(II), Ag(I), Zn(II), Cd(II), and La(III) are characterized via spectroscopic and analytical techniques, such as ultraviolet‐visible (UV–vis), infrared (IR), 1H NMR, 13C NMR, mass spectra, powder X‐ray diffraction (XRD), and thermogravimetric (TG) analysis. The spectral analysis indicated that the novel Schiff base may coordinate with the two metal ions. Additionally, the results indicated a square planar geometry for NiL, CuL, and AgL complexes and an octahedral geometry for ZnL, CdL, and LaL complexes. In contrast to the Schiff base ligand, different complex crystallinities were displayed in the powder XRD patterns. Significant thermodynamic parameter values were computed using the Coats–Redfern method which revealed high thermal stabilities of the complexes in the TG analysis. In vitro antibacterial, DNA‐binding, and anti‐inflammatory techniques were used for the biological evaluation. Moreover, to visualize the anti‐inflammatory action of complexes employing the cyclooxygenase enzyme receptor (PDB ID: 6COX), in silico docking was used. The serial dilution method evaluated the new compounds' in vitro antibacterial efficacy against three bacterial and one fungal strain. The results showed that the novel complexes showed remarkable efficiency against the bacterial growth of gram (+ve) Staphylococcus aureus compared to the parent ligand and the control. On the other hand, the ZnL, CdL, and AgL complexes showed excellent bactericidal activity. The inhibition of protein denaturation followed the following sequence: Ibuprofen > ZnL ˃ NiL ˃ AgL ˃ CuL > CdL > LaL > H2L. UV–visible and gel electrophoresis methods were used to examine the metal complexes and their ligand's affinity for binding DNA. An intercalative binding mechanism was suggested by the findings. [ABSTRACT FROM AUTHOR]

A new chlorobenzylidene imine ligand, (E)-1-((5-chloro-2-hydroxybenzylidene)amino) naphthalen-2-ol (HL), and its [Zn(L)(NO3)(H2O)3], [La(L)(NO3)2(H2O)2], [VO(L)(OC2H5)(H2O)2], [Cu(L)(NO3)(H2O)3], and [Cr(L)(NO3)2(H2O)2], complexes were synthesized and characterized. The characterization involved elemental analysis, FT-IR, UV/Vis, NMR, mass spectra, molar conductance, and magnetic susceptibility measurements. The obtained data confirmed the octahedral geometrical structures of all metal complexes, while the [VO(L)(OC2H5)(H2O)2] complex exhibited a distorted square pyramidal structure. The complexes were found to be thermally stable based on their kinetic parameters determined using the Coats–Redfern method. The DFT/B3LYP technique was employed to calculate the optimized structures, energy gaps, and other important theoretical descriptors of the complexes. In vitro antibacterial assays were conducted to evaluate the complexes' potential against pathogenic bacteria and fungi, comparing them to the free ligand. The compounds exhibited excellent fungicidal activity against Candida albicans ATCC: 10231 (C. albicans) and Aspergillus negar ATCC: 16404 (A. negar), with inhibition zones of HL, [Zn(L)(NO3)(H2O)3], and [La(L)(NO3)2(H2O)2] three times higher than that of the Nystatin antibiotic. The DNA binding affinity of the metal complexes and their ligand was investigated using UV-visible, viscosity, and gel electrophoresis methods, suggesting an intercalative binding mode. The absorption studies yielded Kb values ranging from 4.40 × 105 to 7.30 × 105 M−1, indicating high binding strength to DNA comparable to ethidium bromide (value 107 M−1). Additionally, the antioxidant activity of all complexes was measured and compared to vitamin C. The anti-inflammatory efficacy of the ligand and its metal complexes was evaluated, revealing that [Cu(L)(NO3)(H2O)3] exhibited the most effective activity compared to ibuprofen. Molecular docking studies were conducted to explore the binding nature and affinity of the synthesized compounds with the receptor of Candida albicans oxidoreductase/oxidoreductase INHIBITOR (PDB ID: 5V5Z). Overall, the combined findings of this work demonstrate the potential of these new compounds as efficient fungicidal and anti-inflammatory agents. Furthermore, the photocatalytic effect of the Cu(II) Schiff base complex/GO was examined. [ABSTRACT FROM AUTHOR]

The novel diazenyl pyridinone heterocyclic ligands and their Cu(II) complexes have been prepared and characterized utilizing miscellaneous analytical tools, thermogravimetric analysis (TGA), and nuclear magnetic resonance (NMR), Fourier transform infrared (FTIR), electron paramagnetic resonance (EPR), and ultraviolet–visible spectroscopies. The investigated ligands are coordinated in a bidentate fashion through the azo nitrogen atom and the hydroxyl oxygen atom to the Cu(II) center. The EPR spectra indicated that the obtained complexes were monomeric, and density functional theory (DFT) calculations confirmed that the Cu(II) atom adopted a six‐coordinated octahedral geometry. The ligands and their complexes were investigated for their in vitro antimicrobial activity against some types of bacteria and fungi. The antioxidant and anticancer activities of the complexes were also assessed. The results show that these complexes exhibit higher antitumor efficiency and selectivity index against the models of human breast adenocarcinoma cells MCF‐7 and human primary hepatocytes THLE‐2 than the reference standard material doxorubicin. The results were supported by DFT calculations, molecular docking, and Hirshfeld surface analysis. [ABSTRACT FROM AUTHOR]

The ibuprofen Schiff base ligand (HL) [HL=2‐(4‐Isobutyl‐phenyl)‐propionic acid (3‐hydroxy‐naphthalen‐2‐ylmethylene)‐hydrazide] and its Cu (II), Ni (II), Zn (II), and VO (II) chelates were synthesized and characterized. The spectral data revealed that the Schiff base ligand HL coordinated to Cu (II), Ni (II), Zn (II), and VO (II) via azomethine nitrogen and phenolic oxygen. NiL and CuL have square planar geometry, ZnL has octahedral geometry, and VOL has square pyramidal geometry. Density functional calculations (DFT) were performed to examine the molecular geometry. The results of continuous variation and molar ratio methods supported the ratio of (M:L) to be (1:1). The antimicrobial activity of the new Ibuprofen Schiff base ligand and its metal complexes was tested in vitro against bacterial pathogens and fungi. The results revealed that the antimicrobial efficacy follows the order: CuL is the highly inhibitor complex compared with ketoconazole and gentamicin as reference drugs. The in vitro cytotoxic activity of the new ligand and its complexes was also investigated against Hep‐G2, MCF‐7, and HCT‐116 cell lines. The results showed again that CuL is the highest active agent compared with Vinblastine drug. The CT‐DNA binding ability of the studied complexes was evaluated by absorption spectroscopic titrations and gel electrophoresis. The results showed that the complexes have good binding propensity, and their binding constant values were from 3.1 × 105 to 6.1 × 105 M−1. [ABSTRACT FROM AUTHOR]

Sea buckthorn is a temperate bush plant native to Asian and European countries, explored across the world in traditional medicine to treat various diseases due to the presence of an exceptionally high content of phenolics, flavonoids and antioxidants. In addition to the evaluation of nutrients and active compounds, the focus of the present work was to assess the optimal levels for L. plantarum RM1 growth by applying response surface methodology (RSM), and to determine the impact of juice fermentation on antioxidant, anti-hypertension and anticancer activity, as well as on organoleptic properties. Sea buckthorn berries were shown to contain good fiber content (6.55%, 25 DV%), high quality of protein (3.12%, 6.24 DV%) containing: histidine, valine, threonine, leucine and lysine (with AAS 24.32, 23.66, 23.09, 23.05 and 21.71%, respectively), and 4.45% sugar that provides only 79 calories. Potassium was shown to be the abundant mineral content (793.43%, 22.66 DV), followed by copper and phosphorus (21.81 and 11.07 DV%, respectively). Sea buckthorn juice exhibited a rich phenolic, flavonoid and carotenoid content (283.58, 118.42 and 6.5 mg/g, respectively), in addition to a high content of vitamin C (322.33 mg/g). The HPLC profile indicated that benzoic acid is the dominant phenolic compound in sea buckthorn berries (3825.90 mg/kg). Antioxidant potentials (DPPH and ABTS) of sea buckthorn showed higher inhibition than ascorbic acid. Antimicrobial potentials were most pronounced against Escherichia coli BA12296 (17.46 mm). The probiotic growth was 8.5 log cfu/mL, with juice concentration, inoculum size and temperature as the main contributors to probiotic growth with a 95% confidence level. Fermentation of sea buckthorn juice with L. plantarum RM1 enhanced the functional phenolic and flavonoid content, as well as antioxidant and antimicrobial activities. The fermentation with L. plantarum RM1 enhanced the anti-hypertension and anticancer properties of the sea buckthorn juice and gained consumers' sensorial overall acceptance. [ABSTRACT FROM AUTHOR]

Two tetradentate dibasic chelating Schiff base iron (III) chelates were prepared from the reaction of 2,2′-((1E,1′E)-(1,2-phenylenebis(azanylylidene))bis(methanylylidene))bis(4-bromophenol) (PDBS) and 2,2′-((1E,1′E)-((4-chloro-1,2-phenylene)bis(azanylylidene))-bis(methanylylidene))bis(4-bromophenol) (CPBS) with Fe3+ ions. The prepared complexes were fully characterized with spectral and physicochemical tools such as IR, NMR, CHN analysis, TGA, UV-visible spectra, and magnetic moment measurements. Moreover, geometry optimizations for the synthesized ligands and complexes were conducted using the Gaussian09 program through the DFT approach, to find the best structures and key parameters. The prepared compounds were tested as antimicrobial agents against selected strains of bacteria and fungi. The results suggests that the CPBSFe complex has the highest activity, which is close to the reference. An MTT assay was used to screen the newly synthesized compounds against a variety of cell lines, including colon cancer cells, hepatic cellular carcinoma cells, and breast carcinoma cells. The results are expressed by IC50 value, in which the 48 µg/mL value of the CPBSFe complex indicates its success as a potential anticancer agent. The antioxidant behavior of the two imine chelates was studied by DPPH assay. All the tested imine complexes show potent antioxidant activity compared to the standard Vitamin C. Furthermore, the in vitro assay and the mechanism of binding and interaction efficiency of the tested samples with the receptor of COVID-19 core protease viral protein (PDB ID: 6lu7) and the receptor of Gram-negative bacteria (Escherichia coli, PDB ID: 1fj4) were investigated using molecular docking experiments. [ABSTRACT FROM AUTHOR]

In this study, five novel complexes for Co(II), Ni(II), VO(II), Cr(III), and La(III) ions were synthesized from a tridentate NNO monobasic chelating Schiff base ligand, (Z)‐2‐((pyridin‐2‐ylimino)methyl)phenol (HL). Spectral and analytical tools were applied to elucidate the structural compositions of the new compounds. Then, geometry optimization was conducted for all the syntheses by the Gaussian 09 program via the density functional theory method to obtain optimal structures and the most essential parameters. Moreover, the biochemical behaviors of all the syntheses were explored based on the reactivity, which was tested against various cancer cell lines (HepG‐2, MCF‐7, and HCT‐116). The complexes exhibited an interestingly antiproliferative potential against human cancer cell lines, and the cytotoxicities of the new complexes were arranged to follow the order: VOL > CrL > NiL > LaL > CoL > HL. The antioxidant behaviors of the complexes were studied using the DPPH assay, and VOL showed the maximum antioxidant activity, followed by LaL. The antibacterial activities of the HL ligand and its complexes were studied. Moreover, the binding nature of the complexes with calf thymus DNA (CT‐DNA) was investigated based on the spectrophotometric absorption titration, viscosity, and gel electrophoresis methods. The binding ability of the complexes with CT‐DNA was proposed to be just intercalation or replacement mode. The intrinsic binding constant Kb was calculated and arranged based on the following order: VOL (5.2 × 105) > CrL (3.6 × 105) > NiL (3.3 × 105) > LaL (3.0 × 105) > CoL (1.12 × 105) mol−1 dm−3. Docking investigations were performed using the receptors of COVID‐19's main protease viral protein (PDB ID: 6LU7) and Escherichia coli (gram [–ve] bacteria [PDB ID: 1fj4]). [ABSTRACT FROM AUTHOR]

Although salens and imidazoles are well‐studied motifs among bioactive and therapeutic agents, their properties when combined in transition metal complexes are not well developed. To explore the structure/reactivity of this class of compounds, a salen‐based ligand, namely (2,2′‐{1,2‐ethanediylbis[nitrilo(E)methylylidene]}diphenol, S), and its binary (MS) and ternary (MSI) complexes (I = imidazole; M = Co (II), Ni (II), Cu (II), Cd (II), Al (III), and La (III)) have been synthesized and fully characterized by standard physicochemical and theoretical methods. Evidence from structural analysis tools along with DFT modeling revealed an unusual monobasic tridentate salen binding mode, involving the phenolic oxygen, the nitrogen of the azomethine group, and NH group formed via phenol‐to‐cyclohexadienone tautomerization, giving rise to a general molecular formula of MSI complexes as [M(S)(I)2(Cl)] for M (II) = Co, Ni, Cu and Cd or [M(S)(I)(Cl)2] for M (III) = Al and La, respectively. The antimicrobial activities of S, MS, and MSI were screened against several bacterial and fungal strains. Of all tested complexes, CdS and CuSI were the most effective antimicrobials, giving larger inhibition zones than the reference antibiotics. The antimicrobial efficacy for the MS complexes follows the order: CdS > gentamicin > CuS > NiS > CoS > LaS > AlS > S, whereas MSI complex, potencies are ordered as CuSI > gentamicin > CdSI >NiSI > CoSI > LaSI > AlSI > S. In vitro cytotoxicity screening of MSI complexes disclosed that both CuSI and CdSI exhibited higher activity against human liver (Hep‐G2) and breast (MDA‐MB231) carcinoma cell lines than the reference (cisplatin) drug. The satisfactory bioactivities observed for several of these compounds supports the underlying design idea for combining important bioactive motifs for possible therapeutic benefit. [ABSTRACT FROM AUTHOR]

The new Schiff base ligand 2,2′-{(4-chloro-1,2-phenylene)bis(nitrilo(E)methylylidene)}bis(4-bromophenol) (H 2 L) and its VO(II), Zn(II) and ZrO(II) metal chelates have been synthesized and characterized by spectral, powder x-ray diffraction (PXRD), molar conductance, magnetic measurements, thermal and elemental analyses. The molecular geometry of the prepared compounds has been confirmed by applying the theoretical density functional theory calculations (DFT). The analytical data showed that the parent azomethine H 2 L ligand binds to the VO(II), Zn(II) and ZrO(II) ions through both of the two azomethine-N and two phenolic-O groups and adopts distorted octahedral geometry for ZnL(H 2 O) 2 chelate while square pyramidal geometries for VOL and ZrOL chelates. The antioxidant activity of the compounds was also evaluated by using 1,1‐diphenyl‐2‐picrylhydrazyl (DDPH) reduction method and compared with the positive control ascorbic acid. Carcinoma cells such as breast (MCF-7), liver (Hep-G2), colon (HCT-116) carcinoma cell lines and human embryonic kidney 293 cells (HEK-293) were used for in vitro cell proliferation to investigate the anticancer potency of the prepared compounds. The results showed that, the tumor growth is inhibited and dose-dependent according to the following order: VOL > ZrOL > ZnL(H 2 O) 2 > H 2 L. The titled compounds have been also tested for their antimicrobial activity against certain pathogenic bacteria and fungi. The results showed that the H 2 L ligand and its complexes has enhanced antibacterial and antifungal activities. The CT-DNA binding experiments of azomethine chelates showed that, the binding modes are intercalative, and the determined intrinsic binding constants (K b) for the VOL, ZrOL, ZnL(H 2 O) 2 complexes, are in the range 6.1–7.8 × 105 mol−1 dm−3. The docking calculations were performed to probe the nature of binding affinity of the synthesized compounds with human DNA (PDB:1bna). The compounds may be applicable orally in an accurate manner, according to their in-silico intake, delivery, metabolic processes, digestion, and toxic effects (ADME) data. [ABSTRACT FROM AUTHOR]

ABSTRACT Novel imine complexes of ZrO(II), VO(II), and Pd(II) have been synthesized and described using IR, TGA, UV/Vis, and 1HNMR methods, along with powder X‐ray diffraction (XRD), elemental (CHN) analysis, mass spectrometry, molar conductance, and magnetic susceptibility measurements. Conductivity measurements indicate that none of the complexes function as electrolytes. Magnetic susceptibility data confirm the paramagnetism of the VO(II) complex, while ZrO(II) and Pd(II) complexes exhibit diamagnetic properties. Various analyses suggest that divalent cations favor a 1:1 metal‐to‐ligand ratio. Biological studies demonstrated that all complexes possess significant antimicrobial activity against a range of bacterial and fungal strains. We also observed that the antimicrobial potency of the metal complexes follows the order: ZrO(II) > Pd(II) > VO(II) > H2A, with the ZrO(II) complex showing the highest inhibition zone (28 mm) against Gram‐negative bacteria. The antifungal activity against Aspergillus flavus increases in the order VO(II) > ZrO(II) > Pd(II) > H2A. Additionally, the complexes exhibit high cytotoxicity against carcinoma cell lines, including the HepG‐2, when compared with the clinically proven vinblastine. Density functional theory (DFT) calculations were performed to elucidate the equilibrium geometry of the imine ligand and its complexes at the B3LYP level of theory, using the Gaussian 09 program with 6‐311G++(dp) basis set for C, H, O, N, and Cl atoms, and lanl2dz for Pd, V, and Zr atoms. Finally, docking studies revealed potential binding modes at the active sites of bacterial and fungal receptors. [ABSTRACT FROM AUTHOR]

Despite the common use of salens and hydroxyquinolines as therapeutic and bioactive agents, their metal complexes are still under development. Here, we report the synthesis of novel mixed-ligand metal complexes (MSQ) comprising salen (S), derived from (2,2′-{1,2-ethanediylbis[nitrilo(E) methylylidene]}diphenol, and 8-hydroxyquinoline (Q) with Co(II), Ni(II), Cd(II), Al(III), and La(III). The structures and properties of these MSQ metal complexes were investigated using molar conductivity, melting point, FTIR, 1H NMR, 13C NMR, UV–VIS, mass spectra, and thermal analysis. Quantum calculation, analytical, and experimental measurements seem to suggest the proposed structure of the compounds and its uncommon monobasic tridentate binding mode of salen via phenolic oxygen, azomethine group, and the NH group. The general molecular formula of MSQ metal complexes is [M(S)(Q)(H2O)] for M (II) = Co, Ni, and Cd or [M(S)(Q)(Cl)] and [M(S)(Q)(H2O)]Cl for M(III) = La and Al, respectively. Importantly, all prepared metal complexes were evaluated for their antimicrobial and anticancer activities. The metal complexes exhibited high cytotoxic potency against human breast cancer (MDA-MB231) and liver cancer (Hep-G2) cell lines. Among all MSQ metal complexes, CoSQ and LaSQ produced IC50 values (1.49 and 1.95 µM, respectively) that were comparable to that of cisplatin (1.55 µM) against Hep-G2 cells, whereas CdSQ and LaSQ had best potency against MDA-MB231 with IC50 values of 1.95 and 1.43 µM, respectively. Furthermore, the metal complexes exhibited significant antimicrobial activities against a wide spectrum of both Gram-positive and -negative bacterial and fungal strains. The antibacterial and antifungal efficacies for the MSQ metal complexes, the free S and Q ligands, and the standard drugs gentamycin and ketoconazole decreased in the order AlSQ > LaSQ > CdSQ > gentamycin > NiSQ > CoSQ > Q > S for antibacterial activity, and for antifungal activity followed the trend of LaSQ > AlSQ > CdSQ > ketoconazole > NiSQ > CoSQ > Q > S. Molecular docking studies were performed to investigate the binding of the synthesized compounds with breast cancer oxidoreductase (PDB ID: 3HB5). According to the data obtained, the most probable coordination geometry is octahedral for all the metal complexes. The molecular and electronic structures of the metal complexes were optimized theoretically, and their quantum chemical parameters were calculated. PXRD results for the Cd(II) and La(III) metal complexes indicated that they were crystalline in nature. [ABSTRACT FROM AUTHOR]

Designing new metal-based molecular antibiotics is an efficient approach to overcome the growing threat of antimicrobial resistance. In this paper, novel Cr(III), Fe(III) and Cu(II) complexes comprising substituted aryl imidazole ligand (MSEB), namely (2-(1-(2-hydroxyethyl)-4,5-diphenyl-1H-imidazole-2-yl)(4-bromophenol)) have been synthesized and characterized using infra-red (IR), ultraviolet-visible (UV-Vis) and 1H, 13C NMR spectroscopic techniques, together with elemental (CHN) and thermogravimetric analyses, molar conductance, and magnetic susceptibility measurements. The combined results along with the DFT calculations revealed a 1:1 (M: L) stoichiometric ratio and the complexes adopted distorted-octahedral geometries to afford [Cr(MSEB) Cl 2 (H 2 O) 2 ], [Fe(MSEB)(NO 3) 2 (H 2 O) 2 ] and [Cu(MSEB) Cl (H 2 O) 3 ] respectively. Biological studies showed that all complexes exhibited powerful antimicrobial activity against various strains of bacteria and fungi, S. aureus (+ve), E. coli (-ve) and P. aeruginosa (-ve) bacteria and T. Rubrum , C. albicans , and A. flavus fungi. Moreover, the three metal-complexes showed high in vitro cytotoxicity against Colon (HCT-116), Breast (MCF-7), and hepatic cellular (HepG-2) carcinoma cell lines, with MSEBCu complex being the most cytotoxic one. Finally the binding interactions of the complexes with CT-DNA were explored using UV-Vis spectroscopy, viscosity and gel electrophoreses measurements. Image 1 • Some new aryl imidazole complexes were synthesized. • The investigated compounds were characterized via various physicochemical tools. • Antimicrobial activities of the prepared compounds were screened. • Interaction of the prepared complexes with CT-DNA was investigated. • Anticancer activities of the prepared ligand and its complexes were checked. [ABSTRACT FROM AUTHOR]

A simple highly adjustable and effective synthesis of aryl imidazole ligand HL namely (2-(1-butyl-4,5-diphenyl-1H-imidazole-2-yl) (4-bromophenol) was discussed where it was prepared by cyclo condensation of 5-bromo-2-hydroxybenzaldehyde, benzil and butan-1-amine. Three new Cr(III), Fe(III) and Cu(II) coordination compounds of aryl imidazole ligand were synthesized. The multi-substituted aryl imidazole ligand (HL) and its coordination compounds were characterized via a wide range of spectroscopic and analytical tools such as 1H NMR and 13C NMR, infrared (IR) and UV–Vis spectrophotometry, conductivity and magnetic measurements. The crystal and molecular structure of aryl imidazole ligand HL were discussed by using maXus. The structure of the titled aryl imidazole ligand HL and its metal coordination compounds were discussed theoretically by using Gaussian 09 program at the B3LYP/LANL2DZ level of theory. The obtained data showed that the new compounds have 1:1 M ratio (metal: ligand) and non-electrolytes in nature. The newly prepared [Cr(L)Cl 2 (H 2 O) 2 , [Fe(L)(NO 3) 2 (H 2 O) 2 and [Cu(L)Cl(H 2 O) 3 coordination compounds have a distorted-octahedral geometry. Density Functional Theory (DFT) calculations have been carried out to investigate the equilibrium geometry of the ligands and its coordination compound using Gaussian 09 program at the B3LYP/LANL2DZ level. Moreover, the new compounds were tested against the selected species of microorganism namely Staphylococcus aureus (+ ve), Pseudomonas aeruginosa (-ve), Escherichia coli (-ve) and also against Candida albicans , Aspergillus flavus and Trichophyton Rubrumin. The result revealed that new compounds showed high efficacy towards the growth inhibition of the selected pathogenic microorganism. Moreover, the interaction of the new coordination compounds with CT-DNA was studied by absorption spectra, gel electrophoresis and viscosity techniques. The result showed that the interaction of the new coordination compounds with CT-DNA is an intercalative binding mode. Furthermore, the growth inhibitory effects of the new compounds were tested against Hep-G2, MCF-7 and HCT-116 cell lines. Moreover, all complexes exhibited stronger cytotoxicity effect on the outgrowth of different types of carcinoma cells, than their corresponding imidazole ligand and follow the order: CuL > CrL > FeL > HL. Image 1 • Three new CrL, FeL and CuL compounds were synthesized. • The suggested structures were confirmed by physicochemical and spectral techniques. • The new compounds were tested against selected species of microorganism. • The interaction mode of the new compounds with CT-DNA is an intercalation. • Anticacer potency was tested against Hep-G2 cell line, MCF-7 cell line and HCT-116 cell lines. [ABSTRACT FROM AUTHOR]