1. Antigenic assay for protein C determination in horses.
- Author
-
Welles EG, Prasse KW, Duncan A, and Morris MJ
- Subjects
- Animals, Antibodies, Immunoelectrophoresis veterinary, Protein C immunology, Protein C isolation & purification, Antigens blood, Horses blood, Protein C analysis
- Abstract
An antigenic assay was developed for determination of protein C in horses. Protein C, a natural, vitamin K-dependent anticoagulant component in blood, was isolated from equine plasma, a specific antibody was produced in goats, and a rocket electroimmunophoresis assay was established. Tests were performed to verify the identity of the isolated protein C and to determine the purity of the antibody. Protein C antigen was measured in plasma from 34 clinically normal horses, and values were compared with amidolytic function values. The mean (+/- SD) values for the 2 test methods were similar (antigen content, 104.5 +/- 13.8%; amidolytic activity, 104.6 +/- 17.5%), but the correlation coefficient was 0.1. Four horses given Na coumarin had markedly decreased plasma protein C amidolytic activity and minimal decrease in protein C antigen content.
- Published
- 1990